31results about How to "Good clinical promotion value" patented technology

The invention provides a detection method for a non-small cell lung cancer peripheral blood circulating tumor cell PDL1 gene. The method comprises the steps that a, a peripheral blood sample from a non-small cell lung cancer is subjected to membrane filtration, and peripheral blood circulating tumor cells are obtained; b, a filter membrane obtained in the step a is fixed; c, the filter membrane obtained in the step b is detected in situ to determine the PDL1 expression condition; if PDL1 monochrome positive cells are detected in the step c, and HE dyeing is further used for identifying circulating tumor cells for the filter membrane.

The present invention discloses a blood type detection method based on a membrane structure. According to the method, a filtration membrane allowing a single red blood cell to pass through is selected as a core material, blood type antibodies are bound to the filtration membrane, a water absorption material is surrounding the periphery of the filtration membrane, red blood cells with a certain concentration are added on the filtration membrane surface in a dropwise manner during detecting, rinsing is performed with a rinsing liquid, the red blood cells with no antigen-antibody binding exude along with the rinsing liquid, the red blood cells with the antigen-antibody binding are remained on the membrane surface so as to form the visible red clumps, and an operator can judge the result according to whether the red blood cells form the visible red clumps on the filtration membrane so as to determine the blood type of red blood cells. The method of the present invention has beneficial effects of short detection time and objective result.

The invention relates to a method of general-purpose primer PCR that is an immune catching method for detecting bacteria. Coating is carried out based on idiosyncratic antibody of various bacteria. With it being closed, the coated antibody is added into the bacteria solution at 36-38 deg.c for immune catching. Then heat denaturation releases template, and cracking liquid is extracted as PCR reaction template. Finally, using general-purpose primer of bacteria gene 16S rRNA to carry out PCR. The invention combines specificity of antigen-antibody with PCR, which has powerful rate of increasing production, to build iUPPCR for detecting pathogenic bacteria. The invention can catch idiosyncratic bacteria and increase caught bacteria so as to reach the goal for rapid detecting idiosyncratic bacteria. The invention possesses features of good repetitiveness, high sensibility and better specificity. It can detect almost any bacterium.

The invention relates to the field of biological detection, in particular to a forest encephalitis virus IgM antibody ELISA detection kit and a preparation method thereof. The kit is particularly suitable for early auxiliary diagnosis of forest encephalitis viruses in China, has the advantages of high specificity, high sensitivity, fast detection, convenient operation, low cost and the like, and is suitable for clinical promotion.

The invention discloses a traditional Chinese medicine composition for treating erectile dysfunction of middle-aged and old men, as well as application thereof. The traditional Chinese medicine composition for treating erectile dysfunction of middle-aged and old men is prepared from the following raw materials in parts by weight: 6-10 parts of ginseng, 120-180 parts of hairy holly roots, 1 part of stigma croci, 30-60 parts of panax notoginseng, 60-90 parts of raw radix astragali, 30 parts of fructus cnidii, 30 parts of fructus lycii, 30 parts of herba epimedii, 24 parts of cynomorium songaricum, 30 parts of radix rehmanniae preparata, 24 parts of radix bupleuri, 18 parts of fructus aurantii immaturus, and 30 parts of tribulus terrestris. The traditional Chinese medicine composition has the effects of tonifying qi, promoting coronary circulation as well as tonifying liver and kidney; and moreover, the traditional Chinese medicine composition also has a good curative effect on improving clinical symptoms of erectile dysfunction of middle-aged and elderly people as well as diseases refractory to existing medicines, such as intractable erectile dysfunction and the like. Thus, the traditional Chinese medicine composition has certain clinical popularization value.

The invention discloses a pharmaceutical composition of fingolimod hydrochloride and curcumol and application in the preparation of an anti-oral-cancer medicine. The fingolimod hydrochloride is used combined with curcumol to obtain an unexpected synergistic effect on oral cancer treatment. Compared with independently using any one of the fingolimod hydrochloride and the curcumol, the pharmaceutical composition has higher functions of inhibiting oral cancer cell proliferation, migration and significantly inducing apoptosis, can be made into a variety of preparation forms, and has a good clinical promotion effect and development prospects with a view to clinical treatment of oral cancer patients in the future. According to the principle of the pharmaceutical composition, the combination useof fingolimod hydrochloride and the curcumol may activate the expression of various pro-apoptotic proteins of mitochondrial BH3-only, the apoptosis of the oral cancer cells is synergistically and significantly induced, and meanwhile, the combination of the two medicines further cause cell cycle arrest on the oral cancer cells and inhibit the migration and the proliferation of the oral cancer cells.

The invention discloses a medical surgery nursing bag. The medical surgery nursing bag comprises a nursing bag body, a first drawer, a bag cover, surgical knives and a gauze roll, wherein a lifting handle is mounted at the top of the nursing bag body; the bottom of the nursing bag body is connected with the bag cover; a pair of gloves is fixed on the bag cover; drawer fixing plates are mounted at two sides of the nursing bag body; a second drawer is mounted below the first drawer; an alcohol tank is arranged at the right side of the first drawer; an alcohol cotton tank is arranged between a pair of tweezers and the alcohol tank; the surgical knives are fixed at the left side of the second drawer through elastic limiting belts; a pair of medical forceps is fixed at the right side of the second drawer; the gauze roll is stored at the left side of a third drawer; medical needles are fixed in the middle of the third drawer through an elastic limiting belt; a medical thread is mounted at the right side of the third drawer. According to the medical surgery nursing bag disclosed by the invention, the time of doing preparation work of nursing staff is shortened in a utilization process and the missing of objects is avoided, so that the medical care quality is easy to improve and the medical surgery nursing bag has a good clinical popularization value.

The invention provides a secondary fixing method of an indwelling needle. The indwelling needle comprises a Y-shaped indwelling needle and an adhesive pressing tape, wherein the Y-shaped indwelling needle comprises a Y-shaped catheter, an attached tube and a heparin cap. The secondary fixing method comprises the following steps: S1, performing puncture treatment on a patient by using a needle headof the Y-shaped indwelling needle; S2, cutting a medical adhesive tape to form the adhesive pressing tape, wherein the adhesive pressing tape is an I-shaped adhesive tape and/or a linear adhesive tape for later use; S3, enabling the I-shaped adhesive tape to penetrate through the heparin cap and/or the linear adhesive tape to adhere and press the Y-shaped catheter; and S4, fixing an extension tube of the Y-shaped indwelling needle by using the adhesive pressing tape. The secondary fixing method of the indwelling needle is standard in fixing mode, simple, easy to operate, firm in fixing and reasonable in tightness.

The invention belongs to the field of genetic engineering, and particularly relates to application of PF-4708671 to preparation of drugs for reversing drug resistance of non-small-cell lung cancer toosimertinib. By constructing a non-small-cell lung cancer cell strain with the drug resistance to the osimertinib and fluorescent in-situ lung cancer mouse model, it is found that the PF-4708671 can achieve the effect of reversing the drug resistance of lung cancer cells to the osimertinib at the cell and animal levels.

The invention belongs to the field of gene engineering, and particularly relates to an application of NDUFA4L2 in preparation of medicines for reversing breast cancer herceptin resistance. By constructing a breast cancer stable cell strain resistant to herceptin, NDUFA4L2 can play a role in reversing the drug resistance of HER-2 positive breast cancer cells to herceptin at the cellular level. According to the invention, firstly, whole genome analysis is carried out on herceptin sensitive or drug-resistant cells, 487 probes are adopted in total, FDR correction is carried out, 453 differentially expressed genes are found, NDUFA4L2 is the gene with the most significant difference, and the sensitivity to herceptin is regulated and controlled through energy metabolism and oxidative stress level of NDUFA4L2. When being applied clinically, the herceptin gene can contribute to formulation of a treatment scheme of a clinician, prognosis judgment and become a target for research and development of related drugs in the future, the curative effect of herceptin is improved, and the herceptin has a good clinical popularization value.

The invention relates to a new use of drug for improving Dicer expression. The drug is used for the application in preparing drugs for preventing and treating colitis or colon cancer. The colitis is selected from inflammatory bowel disease, the inflammatory bowel disease is ulcerative colitis and Crohn disease, and the colon cancer is colitis related colon cancer. The drug for improving Dicer expression is selected from anastrozole, a salt of anastrozole, a metabolite of anastrozole, and a derivative of anastrozole.

The invention discloses a traditional Chinese medicine for treating chronic pharyngitis. The traditional Chinese medicine contains the following components in mass by weight: 8-20g of radix pseudostellariae, 10-20g of rehmannia glutinosa, 8-20g of cornus officinalis, 5-15g of blackberrykiky rhizome, 5-15g of oroxylum indicum, 5-15g of tinospora capillipes gagnep, 5-15g of periostracum cicada, 5-15g of bombyx batryticatus, 8-20g of poncirus trifoliate, 5-15g of thunberg fritillary bulb, 5-15g of caulis perllae, 3-10g of liquorice, 5-15g of platycodon grandiflorum, 8-18g of roasted loquat leaf, 5-15g of roasted michaelmas daisy and 5-15g of radix clematidis. The traditional Chinese medicine disclosed by the invention has the effects of clearing heat and removing toxicity, clearing lung and relieving sore throat and stopping pain and diminishing inflammation, and has an obvious treating effect for the chronic pharyngitis.

The invention provides a net-like basket for stone removal under a choledochoscope. The basket includes a stone-removal net-like basket body, grasping forceps and a microwave line; a net-like basket pipe sleeve of the stone-removal net-like basket body is internally provided with a grasping-forceps hole and a microwave line hole which are communicated with the front end of the net-like basket pipe sleeve; the two sides of the net-like basket pipe sleeve are connected with a grasping-forceps catheter and a microwave line catheter, an inner hole of the grasping-forceps catheter is communicated with the grasping-forceps hole in the net-like basket pipe sleeve, the forcep ends of the grasping forceps and the electric head end of the microwave line are arranged on the outer side of the net-like basket pipe sleeve, the other end of the grasping forceps stretches to the outer side of the grasping-forceps catheter through the grasping-forceps hole and the grasping-forceps catheter, and the other end of the microwave line stretches to the outer side of the microwave line catheter through the microwave line hole and the microwave line catheter. According to the net-like basket for stone removal under the choledochoscope, stone can be effectively broken, the stone or the broken stone is taken out, bile duct injury is avoided, and the operation time is saved. The defects of the prior art are overcome, and the invention belongs to the field of biliary tract stone removal.

The invention discloses a traditional Chinese medicine oral liquid for treating anemia, made from, by weight, 18-26 parts of Radix Astragali, 14-22 parts of Radix Rumicis Nepalensis, 10-15 parts of Flos Leonuri, 16-24 parts of Caulis Spatholobi, 11-17 parts of Radix Salviae Miltiorrhizae, 9-16 parts of Herba Agrimoniae, 8-14 parts of Radix Polygoni Multiflori Preparata, 5-10 parts of Fructus Ligustri Lucidi, 7-13 parts of Cordyceps, 13-21 parts of Flos Cucurbitae, 17-23 parts of Fructus Lycii, 16-24 parts of Folium Agastaches, 15-25 parts of Radix Angelicae Sinensis, 12-18 parts of Fructus Mori, 10-15 parts of Herba Ecliptae, 20-30 parts of Radix Paeoniae Alba, 17-28 parts of Fructus Jujubae, 14-22 parts of Rhizoma Polygonati, 13-19 parts of Radix Potentillae Anserinae, and 16-24 parts of Radix Rehmanniae Preparata. Discrimination composing is carried out by using essence boosting and marrow replenishing, qi benefiting and yin nourishing, and blood enriching and nourishing as therapeutic principles in allusion to the pathogenesis of anemia according to the differentiation theory in traditional Chinese medicine, such that treating both symptoms and root causes is achieved, the therapeutic effect is significant, acting speed is high, the treatment course is short, it is possible to effectively promote the blood-producing function of marrow and stimulate a blood-producing system to increase human erythrocyte and hemoglobin level, and the traditional Chinese medicine oral liquid is effective in treating and preventing anemia and has significant clinical application value.