The invention discloses a medical sterile-isolation protection sleeve, and relates to the field of medical instruments. The medical sterile-isolation protection sleeve comprises a pre-folded single-layer protection sleeve body and a supporting sleeve, wherein the supporting sleeve is matched with the protection sleeve body in an embedded mode and used for maintaining the three-dimensional model of the protection sleeve body and guiding the protection sleeve body to turn so as to wrap a mechanical arm changeful in angle at a time. An inner opening is formed in the end, making contact with an operation sterile region, of the protection sleeve body, and an outer opening is formed in the other end, which is connected with equipment and is close to a contamination region, of the protection sleeve body. Multi-blade fan-shaped supports are arranged at the two ends of the supporting sleeve and used for maintaining the folded or compressed state of the protection sleeve body, a binding strap used for tightening the inner opening is arranged on the edge of the periphery of the inner opening, and a spring fastener penetrates through the binding strap. The medical sterile-isolation protection sleeve is matched with the mechanical arm of an operation robot and cables of an abdomen microscope, an endoscope, an operation microscope and an ultrasonic unit and can achieve sterile isolation, in the using process, wrapping is convenient, instrument connection and butt joint are facilitated, and preparation time before an operation is shortened.