Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of compounds with enhanced Dicer expression in drug preparation

A drug and low-expression technology, applied in drug combinations, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve problems such as unclear dynamic patterns of Dicer expression

Active Publication Date: 2020-09-22
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dynamic pattern of Dicer expression during colitis-associated carcinogenesis remains unclear

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of compounds with enhanced Dicer expression in drug preparation
  • Application of compounds with enhanced Dicer expression in drug preparation
  • Application of compounds with enhanced Dicer expression in drug preparation

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0137] a. Preparation of protein standard: Take a tube of BSA protein standard and dissolve it in 1.2ml protein standard preparation solution to prepare a standard with a concentration of 25mg / ml. Use of standards for determining protein concentration;

[0138]b. Add 0, 1, 4, 8, 12, 16, and 20ul of standard substances into the 96-well plate, and add the standard substance diluent to make the final volume 20ul, and the corresponding standard substance concentrations are: 0, 0.025 , 0.05, 0.1, 0.2, 0.3, 0.4, 0.5mg / ml;

[0139] c. Add 20ul protein sample to 96-well plate

[0140] d. Add 200ul of BCA working solution to the air where the samples and standards are added, and incubate at 37°C for 30 minutes. The formula of the working solution is: add BCA reagent A and B at a ratio of 50:1 to make an appropriate amount of BCA working solution;

[0141] e. Use a multifunctional microplate reader to measure the absorbance at A562.

[0142] f. Draw a protein standard curve according...

Embodiment 1

[0264] Embodiment 1: Dicer enzyme and colitis and the exploration of colon cancer relation

[0265] 1. The correlation and mechanism between the expression of Dicer and the pathogenesis of colitis and colon cancer

[0266] In order to study the expression of Dicer in precancerous colitis, paraffin-embedded colon samples from 56 patients with inflammatory bowel disease (27 patients with Crohn's disease and 29 patients with ulcerative colitis) and 57 normal subjects were tested. Dicer expression in paraffin-embedded tissue samples, immunohistochemical analysis showed that in inflammatory colon tissues, compared with normal tissue samples, Dicer expression decreased ( figure 1 A-B), in addition, using the frozen inflammatory colon tissues of 46 inflammatory bowel disease patients and 34 normal population tissue samples for analysis, the results showed that Dicer was reduced at the protein level, but not at the mRNA level ( figure 1 C-E); similarly, Dicer protein levels, but no...

Embodiment 2

[0279] Example 2: The effect of anastrozole on hydrogen peroxide-induced cell damage

[0280] Consistent with our previous study that Dicer overexpression promotes DNA repair, Dicer overexpression reduced H 2 o 2 Induced DNA damage, reduced H 2 o 2 induced an increase in cytoplasmic DNA and alleviated the H 2 o 2 Induced expression of IL-6 ( Figure 12 A-C and Figure 13 A-B). By screening the drug library, we found that anastrozole can enhance the expression of Dicer ( Figure 14 A and Figure 15 A). Anastrozole-induced increased expression of Dicer decreased response to H 2 o 2 Sensitivity to induced DNA damage ( Figure 14 B-D and Figure 15 B-D). Thus, treatment with anastrozole reduces H 2 o 2 Induced increased expression of cytoplasmic DNA and IL-6 ( Figure 14 E-F and Figure 16 A-B). Silencing Dicer expression could partially eliminate the effect of anastrozole ( Figure 17 A-C and Figure 18 A-B). Together, these findings suggest that anastrozole...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a new use of drug for improving Dicer expression. The drug is used for the application in preparing drugs for preventing and treating colitis or colon cancer. The colitis is selected from inflammatory bowel disease, the inflammatory bowel disease is ulcerative colitis and Crohn disease, and the colon cancer is colitis related colon cancer. The drug for improving Dicer expression is selected from anastrozole, a salt of anastrozole, a metabolite of anastrozole, and a derivative of anastrozole.

Description

[0001] The present invention claims the priority of Chinese patent application CN202010261825.4, and the contents described in the specification, drawings and claims of the priority document are incorporated into the specification of the present invention in full and are regarded as part of the original description of the specification of the present invention. The applicant further declares that the applicant has the right to amend the description and claims of the present invention based on the priority document. technical field [0002] The invention relates to the field of colitis and colon cancer treatment, in particular to the application of drugs for improving Dicer expression and the pharmaceutical composition for improving Dicer expression in preventing and / or treating colitis and colon cancer. Background technique [0003] Dicer is a key enzyme in the RNAi pathway and is essential for the biosynthesis of microRNAs (miRNAs) and small interfering RNAs (siRNAs). Recen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/4196A61K31/7056A61K45/06A61P1/00A61P35/00
CPCA61K31/4196A61K31/7056A61K45/06A61P1/00A61P35/00A61K2300/00
Inventor 唐开福
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com