The present invention discloses a preserve silk cotton fabric and a preparation method thereof, wherein the preserve silk cotton fabric comprises the following raw materials by weight: 45-60 parts of tencel cotton, 35-65 parts of poultry feathers, 0.5-4 parts of a silane coupling agent KH550, 12-22 parts of chitosan, 0.5-5 parts of spun silk fiber, 0.5-4 parts of bamboo leaf powder, 0.5-4 parts of jojoba oil, 5-12 parts of starch, 0.5-4 parts of mineral oil, 2-8 parts of rosmarinus officinalis, 50-60 parts of ramie fiber, and 5-15 parts of water. The preparation method comprises: adding jojoba oil to water, and adding chitosan under stirring; adding starch, stirring, and carrying out spray drying to prepare modified starch; crushing tencel cotton, poultry feathers, spun silk fiber, ramie fiber and rosmarinus officinalis into fiber particles; mixing and stirring the fiber particles and a silane coupling agent KH550 to prepare a spinning melt; adding the modified starch, bamboo leaf powder and mineral oil; and carrying out melt spinning, carrying out water washing, bleaching, oiling, drying, and weaving to prepare the silk cotton fabric. According to the present invention, the preserve silk cotton fabric has characteristics of antibacterial property, preserve, health, mildew prevention, gas permeation, cooling and sweat absorbing, and further has increased strength, increased gloss and increased softness.