The invention discloses wrinkle removing, anti-aging, skin activating and scar fading repair essence which comprises components in parts by weight as follows: 600-850 parts of sterile water, 60-200 parts of glycerin, 30-80 parts of sodium pyrrolidone carboxylic acid, 30-80 parts of 1,3-butanediol, 3-10 parts of collagen, 5-20 parts of panthenol, 3-10 parts of allantoin, 10-40 parts of hydroxyproline, 2-10 parts of bisabolol, 2-10 parts of hyaluronic acid, 0.5-2 parts of recombinant human epidermal growth factors and 1-5 parts of diazolidinyl urea. The preparation method comprises the following steps: (1), sterile water, glycerin, sodium pyrrolidone carboxylic acid, 1,3-butanediol and collagen are mixed uniformly with allantoin and are heated for 20 min at 85 DEG C; (2), the mixture is stirred for 20 min at the speed of 30 rpm and is cooled; (3), panthenol and hydroxyproline are added when the temperature is 55 DEG C, the mixture is uniformly mixed, residual components are added when the temperature is 40 DEG C, the mixture is uniformly mixed and then homogenized for 30s, and discharging is performed when the temperature is 30 DEG C, and the repair essence is obtained.