50 results about "Type XVII collagen" patented technology

The invention belongs to the technical field of cosmetics, and discloses anti-aging recombinant collagen as well as a preparation method and application thereof. The amino acid sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.1, and the nucleotide sequence of the anti-aging recombinant collagen is as shown in SEQ ID NO.2. The recombinant collagen CaI1 is constructed and expressed by recombining the effective sequences of the I-type collagen, the III-type collagen and the XVII-type collagen, and the recombinant collagen CaI1 is free of cytotoxicity, free of hidden danger of endotoxin and good in safety. The skin care product added with the recombinant collagen CaI1 can promote skin collagen absorption, inhibit skin aging and improve skin elasticity, and has a good application prospect.

The invention provides a recombinant XVII type collagen and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening collagen structural domain sequences of the human XVII type collagen and then combining the sequences. The recombinant human XVII type collagen is consistent with the corresponding part of the amino acid sequence of the human XVII type collagen, does not generate immunological rejection when being applied to a human body, and can be widely applied to the industries of surgical medical treatment and medical beauty. In addition, the recombinant human XVII type collagen is high in stability, and the problem of protein degradation in the process of expressing recombinant protein by pichia pastoris can be avoided. In addition, the recombinant human XVII type collagen also has the effects of high biological activity, high biological safety, promotion of cell proliferation and differentiation and promotion of hair growth, and can be applied to the field of cosmetics or medicines as a functional component.

The invention provides a micromolecular recombinant XVII type collagen as well as a preparation method and application thereof, and relates to the technical field of protein engineering. The novel recombinant human XVII type collagen is obtained by optimizing and screening a plurality of spiral region sequences such as a collagen structural domain, a C-terminal region and a middle region of the human XVII type collagen. The amino acid composition of the recombinant XVII type collagen is consistent with that of a natural collagen alpha1 chain, immunological rejection is not generated when the recombinant XVII type collagen is applied to a human body, the recombinant XVII type collagen can be widely applied to surgical medical treatment and medical beauty industries, the molecular weight of the collagen is small, efficient secretion soluble expression in eukaryotic host cells such as pichia pastoris can be achieved, and the recombinant XVII type collagen has a good application prospect. And industrial large-scale production is carried out. According to the present invention, the verification results show that the micromolecular recombinant XVII type collagen further has basement membrane anti-aging activity, wherein the basement membrane anti-aging activity specifically comprises the improvement of the expression levels of the IV type collagen, the VII type collagen and the XVII type collagen;

The invention discloses a high-transdermal recombinant humanized XVII type collagen as well as a preparation method and application thereof, and belongs to the technical field of synthetic biology. According to the preparation method, transdermal peptide TD-1, different functional fragments of human XVII type collagen and coding genes of His tags are selected to be connected in series, gene sequences are optimized through pichia pastoris codon selection preference firstly, and then the high-transdermal recombinant humanized XVII type collagen is obtained; and then the recombinant humanized XVII type collagen T-COL17-NC16 and the recombinant humanized XVII type collagen T-COL17 are constructed by expression of the recombinant humanized XVII type collagen T-COL17. Effect experiments prove that compared with similar products sold in the market, the skin-care product has more excellent transdermal performance, can improve cell proliferation and migration ability, also has obvious ability of resisting early saccharification product ketoamine and late saccharification product dicarbonyl compounds, better ability of scavenging free radicals and resisting elastase, and can be used for preparing the skin-care product. Moreover, synthesis of melanin in B16-F10 cells can be reduced, and the compound is expected to become a novel functional biological raw material to be applied to the fields of pharmaceutical compositions or skin care products and the like.

The present invention relates to the fields of medicine and cosmetology, and discloses a cell active peptide composition that promotes the regeneration of multi-type collagen structural proteins, its preparation method, and its application in medicine and cosmetology. The composition uses raw materials such as tripeptide-10 citrulline, hexapeptide-9, hydrolyzed sodium hyaluronate, γ-aminobutyric acid, decarboxylated carnosine hydrochloride, acetyl tetrapeptide-3, acetyl tetrapeptide-11, palmitoyl dipeptide-7, and palmitoyl pentapeptide-4. The composition can promote the coordinated expression of skin type I, type III, type IV, type VII, type XVII collagen, fibronectin, elastin, and glycosaminoglycans; prevent collagen cross-linking, maintain triple helix structure, and inhibit collagen degradation. Applied to dermatology and cosmetology, it can accelerate the regeneration of structural proteins such as skin collagen, repair skin damage, reduce wrinkles such as crow's feet, tear groove lines, nasolabial folds, and under-eye lines, solve the skin aging problem caused by the loss of structural proteins such as collagen, and improve skin elasticity and firmness.

The invention provides a recombinant XVII type collagen fragment, and the protein fragment sequence of the recombinant XVII type collagen fragment is shown as SEQ ID No.1. The recombinant XVII type collagen fragment is screened and designed based on a natural collagen sequence, has a triple helix structure, has good biological activity, can effectively exert functions, and has a good promotion effect on cell migration, cell proliferation and cell adhesion. The method for preparing the recombinant XVII type collagen fragment has the advantages that the process steps are simplified, the expression efficiency of the recombinant protein is improved, and the production cost is reduced. The recombinant XVII type collagen fragment provided by the invention has good mechanical properties and biological activity, and can be used for preparing therapeutic drugs and biological materials.

The application discloses an anti-wrinkle composition containing recombinant collagen as well as a preparation method and application thereof, and belongs to the technical field of cosmetics and skin care products. The composition contains the following active ingredients in percentage by mass: 0.01%-20% of recombinant humanized type I collagen, 0.01%-20% of recombinant humanized type III collagen, 0.01%-10% of recombinant humanized type XVII collagen, and 0.01%-20% of space-bred desert algae extract. The three kinds of recombinant humanized collagens and the space-bred desert algae extract form a complex system, and when the total mass ratio is in a specific interval of 1.3:1-4:1, the three kinds of recombinant humanized collagens and the space-bred desert algae extract can synergistically improve the skin barrier function. Based on the synergistic mechanism of the complex system, the composition disclosed by the application can effectively promote the skin moisture retention and anti-wrinkle ability, repair the skin barrier, and is suitable for the development and application of skin care products for anti-wrinkle, skin tightening and skin aging delay.

The invention belongs to the field of gene engineering. In particular to high-stability recombinant XVII type collagen as well as a preparation method and application thereof. The recombinant XVII type collagen is obtained by repeating an amino acid sequence (as shown in SEQ ID NO.1) from a natural human XVII type collagen peptide for 2-5 times. The recombinant XVII type collagen can also be secreted and expressed in a pichia pastoris expression system, the preparation method is simple, and the high-yield recombinant XVII type collagen can be obtained at low cost. The cell proliferation, migration and adhesion promoting activities of the recombinant XVII type collagen are obviously superior to those of commercially available XVII type collagen, a triple helix structure can be formed at room temperature, a 1% aqueous solution has high-temperature stability and moist heat sterilization stability, and the recombinant XVII type collagen can be widely applied to the fields of cosmetics and medical instruments.

This invention discloses a recombinant collagen liposome, its preparation method, and its application. The recombinant collagen liposome comprises 1-10 parts recombinant type XVII collagen, 2-15 parts soybean lecithin, 0.3-2 parts tocopherol, 0.5-7 parts antioxidant, 20-54 parts glycerol, and Dendrobium officinale enzymatic hydrolysate to a total weight of 100 parts. This invention improves the stability and transdermal absorption of recombinant type XVII collagen by encapsulating it in liposomes. The prepared recombinant collagen liposomes not only have a high encapsulation efficiency but also retain excellent barrier repair and anti-wrinkle effects after 8 weeks of storage, making them suitable for use in cosmetics.

The present application relates to the field of biological materials, and particularly relates to a ROS / pH dual-response modified type XVII collagen microneedle patch loaded with bioactive substances and application, the microneedle patch is composed of boric acid modified type XVII collagen, bioactive substances and polymer substrate.Boric acid modified type XVII collagen and polyvinyl alcohol are double-crosslinked through physical and chemical levels to form collagen hydrogel, then load bioactive substance insulin-like growth factor, and form ROS / pH dual-response collagen microneedle patch loaded with bioactive substances with polymer substrate.When applied to the skin, the microneedle tip reaches the area where hair follicle stem cells exist in a painless and minimally invasive manner, and due to the change of active oxygen and pH environment in the skin tissue, the borate ester bond in the collagen hydrogel can be broken, thereby removing active oxygen and releasing the wrapped IGF-1 into the hair follicle stem cells.

The invention belongs to the technical field of cosmetics, and particularly relates to collagen peptide with an anti-aging effect as well as a preparation method and application of the collagen peptide. The amino acid sequence of the collagen peptide is as shown in SEQ ID NO.1, and the nucleotide sequence of the collagen peptide is as shown in SEQ ID NO.2. According to the invention, effective sequences of human III type and XVII type collagen are recombined to construct and express the collagen peptide Sa1, and the collagen peptide Sa1 has obvious antioxidant effect, no cytotoxicity and good safety. The skin care product added with the collagen peptide Sa1 can promote skin collagen absorption and effectively exert the anti-oxidation effect, so that the effects of improving skin elasticity and delaying skin aging are achieved.

The present disclosure provides recombinant type XVII collagen having a triple helix structure and uses thereof, which belong to the field of protein engineering technology. The recombinant type XVII collagen has an amino acid sequence formed by tandem repeats of n or more core units, where n is an integer of 1 or more, and the core unit has the amino acid sequence shown in SEQ ID NO: 1. The present disclosure also provides methods for constructing multiple expression systems and methods for preparing recombinant type XVII collagen. provide.

This invention belongs to the field of biomedical materials and skin / hair repair technology, specifically a recombinant collagen freeze-dried composition targeting hair follicle functional sites, its preparation method, and its application. The composition includes recombinant human collagen, selected from recombinant type III collagen containing a Wnt-binding domain, recombinant type XVII collagen containing an NC16A domain, or a combination thereof; a freeze-drying protectant composed of trehalose and mannitol in a mass ratio of 1:2 to 2:1; and a buffer system, a phosphate buffer with a pH of 6.5-7.5 and a concentration of 5-20 mM. This invention provides precise hair follicle targeting and efficacy far exceeding that of ordinary collagen. It is effective for androgenetic alopecia, telogen effluvium, and diffuse thinning hair loss. It can be used for individuals with scalp micro-damage and sensitive scalps, simultaneously improving the scalp microenvironment and preventing hair loss from the root. Furthermore, its long-term stable effects have been verified over multiple time points.

The present invention discloses a recombinant humanized type XVII collagen, its expression vector and genetically engineered bacteria. In the present invention, 4 functional fragments with high activity are obtained through screening. After optimizing one of the fragments and combining them in a certain order, recombinant humanized type XVII collagen JYC1703 is obtained. Or after optimizing one of the fragments, combining them in a certain order and respectively fusing GPP sequences at the amino terminus and carboxyl terminus, recombinant humanized type XVII collagens JYC1701 and JYC1702 are obtained. After codon optimization, they are amplified and cloned into an expression vector. After enzymatic digestion and linearization, they are transformed into Pichia pastoris competent cells. High-copy recombinants are screened through G418 resistance gradient to obtain Pichia pastoris genetically engineered bacteria expressing recombinant humanized type XVII collagen. The recombinant humanized type XVII collagens JYC1701, JYC1702 and JYC1703 of the present invention exhibit excellent functions of promoting cell adhesion, cell proliferation and migration, and are suitable for preparing products such as skin care or medical devices.

The application belongs to the technical field of genetic engineering, and particularly relates to a recombinant humanized type XVII collagen for promoting hair growth, a preparation method and application. An amino acid sequence of the recombinant humanized type XVII collagen is shown as SEQ ID NO. 1. The recombinant protein retains multiple key functional sites of human type XVII collagen, has high biological activity, can significantly promote the proliferation and migration of fibroblasts, and can effectively promote the hair regeneration of an androgenetic alopecia model animal. Specifically, the hair coverage and hair length of the animal are statistically significantly increased. The recombinant humanized type XVII collagen prepared by the application has good biological safety and application adaptability, and can be widely applied to the fields of drugs, cosmetics and health products for promoting hair growth, and has good industrial application prospects.

A new preparation method of recombinant humanized collagen hydrogel is provided. The method comprises the following steps: reacting recombinant humanized collagen and a dialdehyde cross-linking agent, wherein the concentration of the dialdehyde cross-linking agent is 0.01-0.25%, and the recombinant humanized collagen is one or more of recombinant humanized type II, type III and type XVII collagen, and the recombinant humanized collagen has a triple helix structure. The present application determines the aldehyde cross-linking agent suitable for recombinant humanized collagen, especially the recombinant humanized collagen with a triple helix structure and the optimum reaction conditions.

The invention relates to the technical field of biology, in particular to desmosome-XVII type collagen fusion protein as well as a preparation method and application thereof. The invention provides a recombinant XVII type collagen fusion protein which comprises at least one XVII type collagen functional domain fragment and at least one functional domain fragment of desmosome core glycoprotein, the recombinant human X VII type collagen fusion protein provided by the invention has better cell adhesion activity and cell proliferation promoting activity. The recombinant human XVII type collagen fusion desmosis protein can significantly improve the adhesion between cells in a cell culture environment, and compared with commercial human XVII type collagen, the recombinant human XVII type collagen fusion desmosis protein can improve the effect by several times or more. In the aspect of promoting cell proliferation, the cell division cycle can be effectively shortened, so that the number of cells is rapidly increased within the same time, and the XVII type collagen is remarkably superior to commercial human XVII type collagen, and has wider application potential in the fields of skin care products, cosmetics, medicines, biomedical materials and the like.

The application discloses a kind of polypeptide and collagen-containing composition and its application, the composition includes polypeptide and collagen;The polypeptide includes palmitoyl pentapeptide-4, decarboxyl carnosine, acetyl octapeptide-3 and hexapeptide-11;The collagen is recombinant type XVII collagen;The mass ratio of polypeptide and collagen is 12:10 or 12:20.The application adds recombinant type XVII collagen in polypeptide composition, can significantly improve the permeability of polypeptide composition on the one hand, and can improve the generation of collagen, the compounding of polypeptide composition and recombinant type XVII collagen can achieve synergistic effect, improve COL7A1 and COL17A1 gene relative expression, to enhance the overall anti-aging effect.

The present invention belongs to the field of cosmetics technology and discloses an anti-aging recombinant collagen protein, its preparation method, and application. The amino acid sequence of the anti-aging recombinant collagen protein is shown in SEQ ID NO.1, and the nucleotide sequence is shown in SEQ ID NO.2. The present invention constructs and expresses a recombinant collagen protein, CaI1, by recombining the effective sequences of human type I, type III, and type XVII collagen proteins. The recombinant collagen protein CaI1 is non-cytotoxic, free of endotoxin risks, and has excellent safety. Skin care products containing this recombinant collagen protein CaI1 can promote skin collagen absorption, inhibit skin aging, and improve skin elasticity, and have good application prospects.

The embodiment of the invention discloses recombinant XVII humanized collagen and a preparation method thereof, relates to the technical field of recombinant protein, and aims to solve the technical problem of low preparation efficiency of existing XVII type collagen. The preparation method of the recombinant XVII humanized collagen comprises the following steps: obtaining an amino acid sequence containing an integrin receptor site "RGD" and a binding site "KGHK"; based on the amino acid sequence, obtaining recombinant humanized collagen protein granules; the method comprises the following steps: obtaining recombinant lysine hydroxylase gene plasmids on the basis of a human gene LH2; and obtaining the recombinant XVII humanized collagen on the basis of the recombinant humanized collagen protein plasmid and the recombinant lysine hydroxylase gene plasmid.

This invention provides a highly stable recombinant type XVII collagen, its construction method, and its applications, belonging to the field of bioengineering technology. The invention selects the amino acid sequence of natural human type XVII collagen as the basic unit and repeatedly replicates it to obtain recombinant type XVII collagen. The design process of the recombinant type XVII collagen avoids unstable GXY triplets, eliminates potential matrix metalloproteinase cleavage sites and glycosylation sites, and retains the KGD active sequence, thus reducing the probability of containing easily degraded sites. The sequence fragment of the recombinant type XVII collagen is derived from the full-length sequence of the natural collagen gene, exhibiting excellent solubility, bioactivity, and high stability. It remains stable under high-temperature storage conditions and without alkaline conditions, making it suitable as a raw material for the production of pharmaceuticals, medical devices, biomaterials, or cosmetics, demonstrating significant practicality.

The invention relates to the technical field of biology, in particular to a fibronectin-XVII type collagen fusion protein as well as a preparation method and application of the fibronectin-XVII type collagen fusion protein. The fusion protein comprises at least one XVII type collagen functional domain fragment and at least one fibronectin cell binding domain fragment, the novel COLXVII-FN fusion protein is obtained by carrying out recombinant expression on an XVII type collagen helical region functional fragment and a fibronectin structural domain fragment by utilizing a genetic engineering technology. Through in-vitro cell verification and efficacy test, the fusion protein shows the dual efficacy of collagen and fibronectin, and has obvious cell adhesion promotion and anti-inflammatory activity, the cell adhesion promotion can enhance the intercellular connection, and the anti-inflammatory activity of the fusion protein is helpful to alleviate skin inflammation response. The compound also shows the activity of promoting cell proliferation and adhesion, and accelerates the renewal of cells; in conclusion, the fusion protein has wider application potential in the fields of skin care products, cosmetics, medicines, biomedical materials and the like.

The invention relates to a moisturizing and oil-controlling composition and application thereof. The moisturizing and oil control composition is prepared from sodium pyrrolidone carboxylate, recombinant XVII type collagen and a plant extract. According to the composition, the synergistic effect of the sodium pyrrolidone carboxylate and the recombinant XVII type collagen in the moisturizing and relieving aspects is utilized, so that the composition can help oily skin keep healthy water-oil balance while playing a moisturizing effect, and the oil control effect is improved. Besides, the plant extract is added into the composition, so that water-oil balance of the skin can be further adjusted, and the sodium pyrrolidone carboxylate and the recombinant XVII type collagen are promoted to play the effects of relieving, moisturizing and controlling oil.

The application provides a type XVII collagen truncated peptide and application thereof in hair loss prevention products, and belongs to the technical field of peptide products. The type XVII collagen truncated peptide provided by the application can significantly improve the antioxidant capacity of hair follicles, effectively enhance the structural stability of hair follicles, strongly strengthen the hair root toughness, and obviously delay the aging process of scalp cells, thereby synergistically improving the hair loss problem from multiple dimensions. The product provided by the application replaces large proteins with small molecule peptides, solves the technical obstacles of traditional collagen, such as difficult transdermal absorption and easy degradation, and provides a new solution for active ingredients for preventing hair loss. The application provides a core ingredient with high activity, easy absorption and strong safety for the field of preventing hair loss, and meets the urgent needs of consumers for high-efficiency hair loss prevention products. The application realizes the integration of the triple functions of "preventing hair loss, fixing hair and resisting aging" by using industrialized peptide technology, and promotes the upgrading of hair loss prevention products to high efficiency, convenience and universality.

The invention relates to the technical field of recombinant humanized type A (X < VII >) collagen, and particularly discloses construction of a recombinant humanized type A XVII collagen strain as well as preparation and application of a product of the recombinant humanized type A XVII collagen strain. The recombinant bacterium for efficiently synthesizing the humanized A-type and XVII-type collagen is obtained, and the humanized collagen produced by using the recombinant bacterium does not contain any gene sequence of a non-human collagen source. In addition, the collagen prepared by the method has excellent water solubility and stability as well as good hydrophilicity and moisture retention, and has a good application prospect in the fields of delayed treatment of alopecia and the like. Therefore, the preparation method of the recombinant humanized type A (X VII) collagen provided by the invention has a relatively good industrial application prospect.

This invention discloses a biomimetic extracellular matrix composition, its preparation method, application, and cosmetics, belonging to the field of cosmetic technology. The composition comprises, by weight percentage, the following components: Type I collagen: 0.01%-0.5%, Type III collagen: 0.01%-0.3%, Type VII collagen: 0.005%-0.2%, Type XVII collagen: 0.005%-0.2%, elastin: 0.01%-0.3%, fibronectin: 0.005%-0.2%, moisturizing ingredients: 0.2%-2.0%, repair factors: 0.061%-0.8%, polypeptide ingredients: 0.02%-0.8%, β-Xylane: 0.5%-3.0%, excipients: 0%-5%, and the balance being water. This invention uses four types of collagen, elastin, and fibronectin as the core structure, and combines them with moisturizing ingredients, repair factors, and peptides to form a complete synergistic system. This system forms a multi-pathway, multi-level synergistic enhancement system that can biomimetically reconstruct the extracellular matrix structure of the entire skin layer, achieving six effects in one: anti-aging, firming, wrinkle reduction, repair, moisturizing, and brightening.

The invention provides a preparation method and application of a collagen and Ectoine eutectic, collagen and Ectoine are sequentially put into a solvent to be uniformly mixed, and then the mixture is dried at the temperature of 40-50 DEG C to obtain the collagen and Ectoine eutectic. The invention finds that recombinant XVII type collagen and Ectoine can form a new supramolecular eutectic substance in a non-covalent bond binding manner. The supramolecular co-crystal has the effects of improving solubility and compatibility, improving efficacy absorbability, enhancing stability, improving safety and the like. Through the synergistic effect of the Ectoin and the collagen, the skin permeability can be improved, and the skin care effect and the anti-inflammatory effect can be synergistically enhanced.

The present invention specifically relates to a recombinant type 17 collagen with a transdermal effect, a preparation method thereof, and an application thereof. The amino acid sequence of the recombinant type 17 collagen provided by the present invention is selected from one of SEQ ID NO.19, SEQ ID NO.20, SEQ ID NO.21, SEQ ID NO.22, SEQ ID NO.23, SEQ ID NO.24, SEQ ID NO.25, and SEQ ID NO.26. The present invention analyzes fragments of natural type 17 collagen, screens out fragments of multiple active sites, further splices and modifies them, and finally obtains a recombinant protein with good biological activity and transdermal effect. Moreover, the present invention realizes the expression of soluble target protein, and the subsequent purification is simple and convenient, which meets the requirements of industrial production and lays the foundation for further expanding the application of recombinant collagen.

The invention belongs to the technical field of medical cosmetology and tissue regeneration, and particularly relates to a platelet-rich plasma vacuum blood collection tube containing biotin and recombinant humanized XVII type collagen as well as a preparation method and application of the platelet-rich plasma vacuum blood collection tube. Separating the glue layer; an anticoagulant solution layer; the interior of the tube body is vacuumized, and the preset sequence of the functional solution layer, the separation gel layer and the anticoagulant solution layer is configured as follows: after blood is introduced and centrifuged, biotin and recombinant XVII type collagen can migrate from the bottom of the tube and are specifically compounded in a platelet rich plasma (PRP) layer above the separation gel layer; and forming a PRP composite liquid layer different from the red blood cell layer, the separation gel layer and the plasma layer. According to the invention, the time is saved, the pollution risk is reduced, the risks of immunological rejection and disease transmission are avoided, the immunogenicity and virus pollution risk are overcome, the stability and quality uniformity are ensured, and the method accords with the production specifications of medical apparatuses and instruments.