43results about How to "Preserve biological activity" patented technology

Apparatus and methods are disclosed for treating a sample by selectively controlling sonic energy and/or selectively controlling the location of the sample relative to the sonic energy.

The invention discloses a novel chondrocyte epimatrix membrane and a preparation method thereof. The membrane contains cell epimatrix ingredients which have bioactivity and cell activity, wherein the cell epimatrix comprises the following main ingredients in percentage by weight: 80 to 95 percent of bionic type II collagen in an acellular cartilage matrix and 10 to 20 percent of hyaluronic acid, 10 to 20 percent of aminopolysaccharide and the like which serve as a chondrocyte epimatrix; the chondrocyte epimatrix biological membrane prepared from the acellular cartilage matrix has the bionic chondrocyte epimatrix ingredients, is excellent in cell consistency, does not arouse immunological rejection of host cartilage tissue, can be used for repairing the damage of hyaline cartilage tissue of joints and reconstructing the hyaline cartilage tissue, and also can be used for a seed cell inoculating vector and a growth factor sustained-release vector; and the chondrocyte epimatrix contains blood vessel inhibiting factor ingredients, so the membrane also can be used for preventing the conglutination of tissue such as muscle tendons.

The invention discloses freeze-dried powder for repairing acne marks and scars. The freeze-dried powder is prepared by the steps of: A, preparing raw materials; B, dissolving mannitol, oligopeptide-3, disodium hydrogen phosphate and oligopeptide-2 in water at normal temperature, placing the solution in a 121 DEG C autoclave, and performing sterilization at a constant temperature for 20 minutes to form a solution A; C, sequentially adding sodium dihydrogen phosphate and serum albumin into the solution A, shaking the mixture uniformly, and performing filtration by using a filter membrane to obtain a solution B; D, standing the solution B for 18-36h, and then performing filling; E, performing freeze drying on the solution B after filling; and F, packaging the product after drying. The freeze-dried powder prepared by the invention can achieve multiple repair and protection of a skin damaged by acnes, and can promote skin microcirculation and improve the acne marks to ensure that the skin can recovered to a healthy state.

The invention relates to an ovarian large cortex piece vitrified cryopreservation protection liquid and a cryopreservation method thereof. Although the vitrified cryopreservation method is widely applied to experimental animals, a universal cryopreservation method and a standard cryopreservation liquid preparation scheme are absent at present, so that the sized transplantation subjected to vitrified cryopreservation is at a small-size stage, an ovarian small cortex piece has limited follicles, and the service life of the transplanted follicles is influenced. The invention provides vitrified cryopreservation protection liquid suitable for an ovarian large cortex piece, and gonadotropin is added on the basis of the common vitrified liquid. According to the vitrified cryopreservation protection liquid suitable for the ovarian large cortex piece, especially the ovaries of experimental animals, improved variety livestock and wild endangered species in sudden death are conveniently cryopreserved at the most proper osmotic equilibrium time of performing vitrified cryopreservation on the ovarian large cortex pieces of different sizes; and moreover, according to the method, the survival rate of the follicles can be improved, the blood reperfusion time of the transplant is shortened, and the survival rate of the cryopreserved ovarian transplantation in vivo is improved.

The invention discloses a preparation method for abelmoschus esculentus proteoglycan protein xerium, which includes the steps: 1) adding water into abelmoschus esculentus raw materials, crushing the abelmoschus esculentus raw materials with a tissue destructor, adding compound enzyme for enzymolysis and extraction prior to centrifugally removing large residue particles, and performing suction filtration to obtain abelmoschus esculentus proteoglycan protein extracting solution; and 2) adding maltodextrin into the prepared abelmoschus esculentus proteoglycan protein extracting solution, and obtaining the target product, namely the abelmoschus esculentus proteoglycan protein xerium by means of high-pressure homogenization and spray drying. As tested, essential components of the abelmoschus esculentus proteoglycan protein xerium refer to soluble polysaccharide and protein, wherein the content of the polysaccharide is >=75wt%, the content of the protein is >=10wt%, and the moisture content is >=4.2wt%. The abelmoschus esculentus proteoglycan protein xerium can effectively improve the human gastrointestinal function, and is high in active component and fine in physical characteristic, and can serve as raw materials to fill capsules or be tableted so as to produce functional food.

The invention provides a preparation method of cordyceps militaris nano powder, comprising the following steps: 1) separating wild cordyceps militaris paecilomyces, and purifying to prepare cordyceps militaris strain liquor; 2) selecting health and live insect pupae, sterilizing by ozone for later use; 3) infecting the insect pupae sterilized in step 2) by the strain liquor prepared by step 1); 4) performing bionic cultivation on infected insect pupae obtained in step 4) to obtain cordyceps militaris cells integrating pupalcells and sporocarps into a whole; and 5) carrying out microwave drying and nano grinding on the obtained cordyceps militaris cells cultured in step 4) to obtain the cordyceps militaris nano powder. The preparation method of the invention has low investment and low energy consumption, the prepared cordyceps militaris nano powder is not polluted by sterilization process, and the fineness of the powder can reach nano level, thus the powder can keep original functionalactivity and is easily absorbed by a human body.

The invention provides a method for separating and purifying cobra neurotoxin protein through dual-ion exchange chromatography. The method comprises the following steps of: (1) first separation and purification through ion exchange chromatography, namely, a) dissolution of crude cobra venom, and b) separation and purification through an SP-Sephodex-C25 gel column; and (2) second separation and purification through ion exchange chromatography, namely, a) balance of a SourceS(XK5030) column having a column volume of 500 ml by using 1000 ml of liquid A for future use; and b) SourceS(XK5030) column purification, and then collection of the cobra neurotoxin protein purified twice according to a standard purification chromatogram. The method provided by the invention is scientific and rational in process; and no organic reagent, no high-concentration salt and no protein modification method are used in production, so that the biological activity of cobratide can be maintained to an utmost extent. The method provided by the invention is advanced in process and simple to operate; the production period can be greatly shortened, the production time can be saved and the production cost can be reduced; and the method is completely suitable for an industrial production line.

The present invention discloses a novel anti-crop-epiphyte polypeptide and a preparation method thereof. A recombined anti-crop-epiphyte polypeptide is formed by linking gene which codes white candida pheromone and the gene which codes colicin. Compared with the prior anti-crop-epiphyte medicine, the novel anti-crop-epiphyte polypeptide of the present invention has the advantages that the novel anti-crop-epiphyte polypeptide directly forms ionic passage at epiphytic cell membrane to kill the epiphyte, so the killing efficiency is thousands times stronger than the prior anti-crop-epiphyte medicine; the epiphyte can hardly amend the geometrical damage at the cell membrane caused by the polypeptide by the mutation-expression form, so the polypeptide can not induce the epiphyte producing the traditional drug resistance; the polypeptide is a biological preparation and has target killing towards the epiphyte, so the novel anti-crop-epiphyte polypeptide does not have the toxic and side effects of the prior anti-crop-epiphyte medicine.

The invention relates to a production method of quickly-fermented high-umami seafood fermented soybean sauce, including; preparing small-molecular fish protein enzymatic hydrolysate by means of efficient biological enzymatic hydrolysis, and jointly fermenting through the small-molecular fish protein enzymatic hydrolysate with soybean meal and bran to obtain the seafood fermented soybean sauce. The method is low in cost and has a short production cycle, only 30 d, umami of the obtained soybean sauce can be improved, added value of low-value fishes can also be improved, low-value marine animal resources can be comprehensively utilized, and innovation is also made to zero addition of flavorings; meanwhile, the method of the invention is different from common low-salt solid fermented soybean sauces, the addition of the fish protein enzymatic hydrolysate enables the seafood soybean sauce to be healthy, environment-friendly, low in cost, good in property and excellent in umami, and compared with like products, the seafood soybean sauce will be popular with consumers owing to the excellent flavor.

A miniature polypeptide resisting to EB virus tumor, its gene and its recombinant plasmid are disclosed. Its preparing process includes linking the gene for coding and leading peptide with the plasmid carrying the colicin ion channel structure domain gene, transferring engineering bacteria for amplifying, and separating and purifying by His-tag column. It can be used to kill tumor cells.

The embodiment of the invention discloses a wrinkle-removing composition. The wrinkle-removing composition comprises wrinkle-removing composition freeze-dried powder and a solvent, wherein the wrinkle-removing composition freeze-dried powder is prepared from, by mass, 0.001-2% of palmityl tripeptide-1, 0.01-2% of palmityl pentapeptide-4, 0.001-5% of collagen and 91-99.7% of mannitol. The wrinkle-removing composition comprises the wrinkle-removing composition freeze-dried powder, through the preservation of the freeze-dried powder, the bioactivity of polypeptide can be maintained to the greatest extent, and the best effect is achieved. The freeze-dried powder composition and the solvent are separately preserved, and when the wrinkle-removing composition is needed, what is only needed is touniformly mix the freeze-dried powder composition and the solvent. According to the wrinkle-removing composition, expression wrinkles and physiological static wrinkles can be inhibited and desalinatedsimultaneously, and the skin collagen is directly and quickly supplemented, so that the skin gets tightened and smooth.

The present invention relates to freeze dried powder for injection with ginseng stem and leaf extract as main material and its preparation process. The freeze dried powder for injection may be used in treating coronary heart disease and climacteric syndrome, and can strengthen physique and strengthening immunity. It contains ginseng stem and leaf extract in 3-20 weight portions and supplementary material and water in 25-250 weight portions. The preparation process includes adding supplementary material and water into ginseng stem and leaf extract through stirring to dissolve, packing, and freeze drying via quick lowering temperature to -40 to -55 deg.c and pumping to 5-10 Pa, heating to 25-40 deg.c and maintaining for 5-20 hr. The preparation process has less damage of the effective medicine components and stable product quality, and the product has long preservation period and other advantages.

The invention discloses a method for preparing active collagen polypeptides from fresh pig skin. The method comprises the following preparation steps of 1, selection of a material, wherein the fresh pig skin which is qualified in inspection and quarantine is selected and purchased from a regular large meat factory on the market; 2, rough processing of the pig skin, wherein the selected and purchased pig skin is preliminary processed; 3, cleaning of the pig skin, wherein the pig skin after being cut flat is cleaned by adopting a mode of combining mechanical and physical methods; 4, enzymatic hydrolysis of the pig skin, wherein the clean pig skin after shredding and cleaning is placed in an enzymatic hydrolysis reactor, and pure water is added into the enzymatic hydrolysis reactor accordingto the amount of the pig skin; 5, purification separation and concentration, wherein an enzymatic hydrolysate obtained after the enzymatic hydrolysis of the pig skin material is filtered through a filter cloth; 6, drying, wherein a collagen polypeptide concentrate after concentration is placed in a front box of freeze-drying equipment for freeze-drying for 40-50 hour; smashing and packaging.

The invention discloses a preparation method of a multifunctional biological chip. The preparation method comprises the following steps: step one, manufacturing an adhesive layer to obtain the adhesive layer with a through hole; step two, fixing the adhesive layer on a substrate; step three, fixing the AAO film on the base through the adhesive layer, wherein the through hole location of the adhesive layer coincides with a center point of the AAO film; step four, depositing gold-nano-particles on the AAO film; step five, taking off an Au-AAO film with the gold-nano-particles from the substrate;step six, dropwise adding PEG buffer solution at one side of the Au-AAO film with gold-nano-particles; step seven, keeping the Au-AAO film dropwise added with the PEG buffer solution in dark place toobtain a PEG-Au-AAO film for manufacturing a biological chip after modifying the biomolecule. The prepared biological chip prepared through the preparation method disclosed by the invention can quickly detect the specific protein or extracellular vesicles while separating the specific protein or extracellular vesicles, and the operation is simple and efficient. The preparation process is free from using the organic chemical reagent, the pollution on the protein and extracellular vesicles is avoided, the biological activity can be saved, and a use demand on the biological application field issatisfied.

The present invention relates to recipe and making process of one health food with shallot bulb as main material. The health food is prepared with shallot bulb in 55-98 wt% and one of fleeceflower root, notoginseng and red sage in 0.1-45 wt%, and through freeze drying, superfine crushing and other steps. It is prepared into tablet, granule, capsules or other form. It has the functions of regulating blood fat and preventing cardiac and cerebral vascular diseases.

The invention provides a method for adding natural astaxanthin to baked food. The method comprises the following steps: S1, feeding the laying hen with the haematococcus pluvialis algae powder as a raw material, and obtaining the astaxanthin egg rich in natural L-esterified astaxanthin; S2, using astaxanthin egg as a base material, processing the material into mayonnaise, egg yolk powder and egg butter rich in animal astaxanthin under low temperature conditions; S3, astaxanthin egg yolk powder: directly mixing the egg yolk powder obtained in S2 with the flour, and performing operation according to the normal operation process; S4, astaxanthin mayonnaise: directly mixing the mayonnaise obtained in S2 with flour, or being used for post-moulding and seasoning after setting the noodles; S5, astaxanthin egg butter: directly mixing the egg butter obtained in S2 with cream and an oily filler of salad oil; and S6, astaxanthin egg: breaking the astaxanthin egg obtained in S1 and directly mixingthe material with the flour, and performing operation according to the normal operation process. The method ensures better biological activity, avoids fishy smell and improves body absorption rate.

The invention discloses a kit for determining an amino-terminal pro-brain natriuretic peptide. The kit is characterized by comprising a reagent card, a reaction substance and a reaction buffer solution, a detection line of the reagent card is coated with an amino-terminal pro-brain natriuretic peptide monoclonal antibody, and a quality control line of the reagent card is coated with one of a goatanti-chicken IgY antibody, a goat anti-rabbit IgG antibody or a goat anti-mouse IgG antibody. Before the kit is used, a reaction buffer solution is adopted to redissolve a reaction substance, and thena sample to be detected is added so that the amino-terminal pro-brain natriuretic peptide in the sample to be detected fully reacts with the amino-terminal pro-brain natriuretic peptide monoclonal antibody marked by the fluorescent microspheres, and the kit is high in detection accuracy, strong in specificity, good in reproducibility and rapid to operate.

The invention relates to a grinding device suitable for frozen tissue. The grinding device suitable for the frozen tissue comprises a base, wherein a tool accommodating box is arranged in the base, adrawer-type powder collecting box with an open upper part is arranged on the base, a low-temperature crushing box body penetrating up and down is arranged on the upper part of the powder collecting box, dry ice-type low-temperature ice bags are arranged in four side walls of the box body, a drawable screen is arranged at the bottom of the box body, a sealing cover is arranged at the top end of thebox body, a feeding port is fixedly arranged on the sealing cover, the top end of the feeding port is hinged with a sealing feeding cover, a grinding and crushing mechanism is horizontally arranged in the middle of the box body, and the grinding and crushing mechanism is manually driven to realize low-temperature crushing on the frozen tissue. The grinding device suitable for the frozen tissue has the advantages of simple structure and ingenious design, ensures the activity of an experimental sample, reduces the loss of a tissue sample, protects the safety of experimental personnel, and greatly improves the grinding efficiency.

The invention relates to a preparation method for measuring a biological active substance of feed protein of ruminants. The method comprises the following steps of: extracting rumen fluid of cattle or sheep, and filtering the rumen fluid to remove fed residues from the rumen fluid; and mixing liquid obtained by filtering and a freeze drying protective agent according to a ratio of 1:1, and putting the mixture into a vacuum freeze drying machine for freeze drying to prepare biological active substance powder. By the method, the problem that available protein in ruminant feed is measured only in the rumen fluid provided by rumen fistula animals or excrement of the ruminants is solved, so that the nutritional value of feed protein of the ruminants can be evaluated simply, conveniently, quickly and accurately, nutrient substances are provided for animals accurately, and diseases are prevented simultaneously.