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Preparation method of multifunctional biological chip

A biochip, multifunctional technology, applied in the field of preparation of multifunctional biochips, to achieve the effects of improving detection sensitivity and specificity, large specific surface area, and avoiding chemical pollution

Active Publication Date: 2019-04-02
WENZHOU INST OF BIOMATERIALS & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The modification of the structure, morphology and optical properties of the surface of the biochip can improve the detection sensitivity and specificity of the analyte, but there is no accurate and effective method in the prior art to modify the structure, morphology and optical properties of the surface of the biochip. effective retrofit

Method used

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  • Preparation method of multifunctional biological chip

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] A preparation method of a multifunctional biochip, the steps of which are:

[0038] 1) Use a puncher to punch holes on the double-sided adhesive tape to obtain double-sided adhesive tape with an aperture of 3mm;

[0039] 2) the double-sided adhesive with 3mm aperture obtained in step 1) is fixed on the polymethyl methacrylate film to obtain the polymethyl methacrylate film with the double-sided adhesive with 3mm aperture;

[0040] 3) the AAO membrane with a single channel aperture of 50nm is fixed on the polymethyl methacrylate film with the double-sided adhesive tape of 3mm aperture obtained in step 2), to obtain a polymethylmethacrylate with an AAO membrane with an aperture of 50nm Methyl acrylate film, wherein the center point of the AAO film coincides with the center point of the small hole on the double-sided adhesive tape;

[0041] 4) Depositing gold for 50 min on the polymethyl methacrylate film with a pore size of 50 nm obtained in step 3) to obtain gold nanopa...

Embodiment 2

[0048] A preparation method of a multifunctional biochip, the steps of which are:

[0049] 1) Use a puncher to punch holes on the double-sided adhesive tape to obtain double-sided adhesive tape with an aperture of 4mm;

[0050] 2) the double-sided adhesive with 4mm aperture obtained in step 1) is fixed on the polyethylene terephthalate film to obtain the polyethylene terephthalate film with the double-sided adhesive with 4mm aperture;

[0051]3) the AAO membrane with a pore diameter of 20nm is fixed on the polyethylene terephthalate film with a double-sided adhesive tape with a pore diameter of 4mm obtained in step 2), and the AAO membrane with a pore diameter of 20nm is obtained. Polyethylene terephthalate film, wherein the center point of the AAO film coincides with the center point of the small hole on the double-sided adhesive tape;

[0052] 4) Depositing gold for 60 min on the polyethylene terephthalate film obtained in step 3) with an AAO film with a pore size of 20 nm ...

Embodiment 3

[0059] A preparation method of a multifunctional biochip, the steps of which are:

[0060] 1) Use a puncher to punch holes on the double-sided adhesive tape to obtain double-sided adhesive tape with an aperture of 5 mm;

[0061] 2) fixing the double-sided adhesive with a 5mm aperture obtained in step 1) on the silicon wafer to obtain a silicon wafer with the double-sided adhesive with a 5mm aperture;

[0062] 3) the AAO membrane with a single-channel aperture of 100nm is fixed on the silicon wafer with the double-sided adhesive tape with a 5mm aperture obtained in step 2), to obtain a silicon wafer with an AAO membrane with an aperture of 100nm, wherein the center of the AAO membrane The point coincides with the center point of the small hole on the double-sided tape;

[0063] 4) Depositing gold for 30 min on the silicon wafer with the AAO film with a pore size of 100 nm obtained in step 3) to obtain gold nanoparticles;

[0064] 5) Remove the AAO film with a pore size of 100...

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Abstract

The invention discloses a preparation method of a multifunctional biological chip. The preparation method comprises the following steps: step one, manufacturing an adhesive layer to obtain the adhesive layer with a through hole; step two, fixing the adhesive layer on a substrate; step three, fixing the AAO film on the base through the adhesive layer, wherein the through hole location of the adhesive layer coincides with a center point of the AAO film; step four, depositing gold-nano-particles on the AAO film; step five, taking off an Au-AAO film with the gold-nano-particles from the substrate;step six, dropwise adding PEG buffer solution at one side of the Au-AAO film with gold-nano-particles; step seven, keeping the Au-AAO film dropwise added with the PEG buffer solution in dark place toobtain a PEG-Au-AAO film for manufacturing a biological chip after modifying the biomolecule. The prepared biological chip prepared through the preparation method disclosed by the invention can quickly detect the specific protein or extracellular vesicles while separating the specific protein or extracellular vesicles, and the operation is simple and efficient. The preparation process is free from using the organic chemical reagent, the pollution on the protein and extracellular vesicles is avoided, the biological activity can be saved, and a use demand on the biological application field issatisfied.

Description

technical field [0001] The invention relates to a production method, more specifically to a preparation method of a multifunctional biochip. Background technique [0002] Cancer is one of the diseases that seriously affect the quality of human life and threaten human health. How to effectively detect and treat cancer early is still an urgent problem to be solved. The traditional detection method is based on the pathological detection of cells and tissues, which requires surgery or puncture to obtain the corresponding diseased tissue from the patient, which will inevitably cause certain harm to the patient. Moreover, the early symptoms of some cancers are not obvious, and it is easy to miss the best treatment time. In recent years, researchers have found that the dynamic changes of some specific biomacromolecules and extracellular vesicles (such as exosomes, microvesicles, etc.) in body fluids (such as blood, saliva, peritoneal fluid, etc.) can reflect the state and developm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 刘飞邓载安
Owner WENZHOU INST OF BIOMATERIALS & ENG
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