Near-infrared region II polymer fluorescent microsphere and preparation method thereof

Abstract

The invention discloses polymer fluorescent microspheres in the near-infrared II region and a preparation method thereof, wherein the preparation method includes: (1) dissolving the fluorescent dye with a water-immiscible organic solvent so as to obtain a fluorescent dye solution, the fluorescent dye It has the structure shown in formula I; (2) disperse the polymer microspheres in the sodium dodecylsulfonate aqueous solution, so as to obtain the microsphere carrier solution; (3) mix the fluorescent dye solution and the microsphere carrier solution and perform ultrasonic processing, so as to obtain the emulsified mixed solution; (4) swelling the emulsified mixed solution, so that the fluorescent dye solution is immersed in the nanopores produced in the swelling process of the polymer microspheres; and (5) the mixture obtained in step (4) is processed After heating, with the volatilization of dichloromethane, the fluorescent dye crystallizes out and is wrapped in the nanopores, so as to obtain polymer fluorescent microspheres in the near-infrared II region. The fluorescent microspheres have high fluorescence quantum efficiency, strong penetration ability and low background interference during fluorescence detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to polymer fluorescent microspheres in the near-infrared II region and a preparation method thereof. Background technique [0002] As a special functional microsphere, polymer fluorescent microspheres can contain tens of thousands or even hundreds of thousands of fluorescent molecules, which can greatly improve the efficiency of fluorescent labeling, enhance the anti-photobleaching performance of fluorescent molecules, and greatly Improve analytical sensitivity; at the same time, the surface of fluorescent microspheres can be very flexibly modified with various functional groups (such as carboxyl, amino, aldehyde, etc.), which is conducive to covalent coupling with proteins or antibodies and improves the stability and labeling of markers efficiency. At present, fluorescent microspheres have been widely used in labeling, tracing, detection, imaging, immobilized enzymes, immun...

AI Technical Summary

Problems solved by technology

However, the emission of traditional fluorescent microspheres is in the visible light region (the emission wavelength is less than 780nm). When it is applied in the fields of living organisms, cell or tissue imaging, and in vitro diagnosis, it is easy to cause poor fluorescence penetration and strong background fluorescence.

At the same time, due to the generally low fluorescence quantum efficiency and the relatively close excitation and emission wavelengths (usually around 20nm), the analysis sensitivity is reduced, and the requirements for the color filters required for fluorescence detection are very high.

Images