20results about How to "No cytotoxicity" patented technology

The invention belongs to the technical field of medical materials, and particularly relates to a degradable wound dressing and a preparation method thereof. Compared with the prior art, the degradable wound dressing prepared by the preparation method disclosed by the invention is a completely biodegradable Janus composite film, and a hydrophobic inner layer and a hydrophilic outer layer are obtained by selecting different materials; the material PGA with excellent biological performance is utilized, the problem that electrostatic spinning cannot be carried out through pure PGA is solved, PGA and gelatin are creatively mixed, the advantages of PGA and gelatin are combined, and the PGA / Gel electrostatic spinning film which is high in toughness, high in tensile strength, good in biocompatibility and high in medicine carrying efficiency is obtained; meanwhile, the functions of resisting bacteria, resisting inflammation, discharging seepage and promoting angiogenesis are achieved, and the wound healing rate is effectively guaranteed.

This invention relates to the field of oral care products technology, specifically to an artificial enzyme with antibacterial properties, its preparation method, and its application. This technical solution employs a chemical reduction method, where a palladium source reacts in an environment containing surfactants and reducing agents to obtain palladium nanozymes. The obtained palladium nanozymes exhibit uniform morphology, controllable particle size, good dispersibility in water, and good biocompatibility; they have no significant toxic side effects on human gingival fibroblasts, possess highly efficient catalase activity, and demonstrate strong antibacterial and bactericidal effects against oral pathogens, significantly reducing bacterial drug resistance; they can inhibit acid production by oral bacteria, preventing tooth decay; and they retain their original catalytic activity even after multiple cycles of use. Applying the palladium nanozymes of this solution to the manufacture of oral care products can solve the technical problems of unsatisfactory safety and easy development of drug resistance in existing oral care products using antibacterial and anti-caries methods, showing promising application prospects.

The application of 2-[(7-hydroxy-8-methyl-2-oxochrome-4-yl)methylthiosulfase]-4,6-dimethylpyridine-3-carbamate in the treatment of Ebola virus belongs to the field of pharmaceutical technology. This invention addresses the technical problem of the lack of effective anti-Ebola virus drugs in the prior art by providing 2-[(7-hydroxy-8-methyl-2-oxochrome-4-yl)methylthiosulfase]-4,6-dimethylpyridine-3-carbamate, which can effectively inhibit Ebola virus replication within a non-toxic range. The compound exhibits good anti-infective effects against SUDV, BDBV, and TAFV viruses and can be used to prepare safe and effective broad-spectrum anti-Ebola virus drugs.

The application discloses a sustained-release LBQ657 hydrogel capable of treating fibrosis and a preparation method thereof, and belongs to the technical field of material synthesis and biological medicine. The sustained-release LBQ657 hydrogel comprises chitosan, beta-glycerophosphoric acid sodium, distilled water and LBQ657. The w / v concentration of the chitosan solution used for configuration of the hydrogel is 3.3%, the w / v concentration of the beta-glycerophosphoric acid sodium aqueous solution is 56%, and the volume ratio of the chitosan solution to the beta-glycerophosphoric acid sodium solution is 4:1-10:1. The sustained-release LBQ657 hydrogel can quickly form a stable gel and is well adhered to the esophagus after being covered on the surface of the esophagus, can provide a protection for at least 7 days under physiological conditions, can significantly relieve TGF-beta induced fibrosis, and can provide a new idea for esophageal fibrosis treatment by covering the wound and long-term drug release.

The application discloses a kind of degradable microspheres with controllable and preparation method thereof, belong to the technical field of biomedical materials.The base material of the microspheres is methacrylated gelatin, and the degradation time of the microspheres is controlled by regulating the degree of substitution of methacrylated gelatin.The microspheres are prepared by microfluidic and photopolymerization technology, and have uniform size with particle size of 10 μm to 1000 μm.The methacrylated gelatin microspheres with controllable degradation provided by the application can be used for interventional recanalization embolization therapy of substantial organ hemorrhage, realize the time sequence regulation of "hemostasis-degradation-recanalization", and have good clinical transformation value.

The present application relates to the technical field of zinc metal battery, in particular to a multifunctional composite gel electrolyte, its preparation method and application. The present application provides a composite gel electrolyte, which comprises a polymer gel matrix, a zinc salt and a ZnHA filler; wherein the ZnHA filler is a hydroxyapatite material doped with zinc ions. The electrolyte is prepared by polymer dissolution, zinc salt addition, ZnHA dispersion, three-dimensional printing forming, low-temperature gelation and zinc salt solution activation, and has good shear thinning characteristics, self-supporting property and printability. Further, a three-dimensional printed interdigital structure electrode is constructed, and the electrolyte is covered to form an ion transmission channel, and a flexible zinc metal battery is assembled; multiple monomers can be connected in series to increase the output voltage. The flexible battery has excellent ion transmission, cycle stability, flexibility and safety, and can be used in the fields of flexible electronics, wearable devices and intelligent sensing.

The invention discloses a pharmaceutical composition for improving glucose and lipid metabolism disorder by combining N-p-coumaroyl octoamine and rotundine and application of the pharmaceutical composition, and belongs to the field of biological medicine and metabolic disease treatment. In a db / db diabetic mouse model, it is found that NTPC (10 mg / kg) or ROT (10 mg / kg) single drugs can improve hyperglycemia and insulin resistance and reduce blood fat, and low-dosage combination (5 mg / kg of NTPC and 5 mg / kg of ROT) shows the most significant metabolism improvement effect which is obviously better than that of a single drug group. In an in-vitro fatty acid stimulation model, lipid accumulation in cells can be more remarkably reduced through combination of the two drugs, and fatty acid synthesis genes are inhibited.

The application discloses a scylla paramamosain antibacterial polypeptide Spgilstin 10‑33 and application thereof, a molecular formula of which is C 118 H 206 N 42 O 30 S, and an amino acid sequence of the Spgilstin is shown as SEQ ID NO. 01. The application has broad-spectrum antibacterial and antimycotic activities, high bactericidal efficiency, no cytotoxicity, high safety, and can be applied to antibacterial agents, antimold agents and aquatic feed additives as effective components, and has wide application prospects.

The invention discloses hydrogel capable of releasing growth factors after heat absorption and contraction and a preparation method of the hydrogel, and belongs to the field of biomedical materials. The hydrogel is of a three-layer composite structure, wherein the first layer is a sodium alginate-chitosan polyelectrolyte semi-interpenetrating network layer and serves as a compact protective layer; the second layer is a bacterial cellulose-sodium alginate high-toughness layer and is used as a bearing layer and a shrinkage driving layer; and the third layer is a basic fibroblast growth factor (bFGF)-loaded poly (butylene adipate / terephthalate) (PBAT) electrostatic spinning film layer and is used as a medicine storage cavern. According to the invention, the characteristic that the bacterial cellulose / sodium alginate layer can generate reversible heat absorption volume shrinkage at a specific temperature is used as a physical driving force to extrude and promote the bFGF in the third layer of PBAT fiber membrane to directionally release outwards.

The utility model relates to an extramedullary positioning far-end osteotomy module based on 3D printing, which belongs to the technical field of knee joint surgical instruments and comprises a far-end osteotomy module body, the far-end osteotomy module body is mounted on a sliding rod of a guide positioning plate, an elastic locking structure is arranged on the far-end osteotomy module body and located on one side of the sliding rod, and the elastic locking structure is connected with the far-end osteotomy module body. The elastic locking structure comprises a pressing end and a clamping end, the fixing side of the clamping end is fixed to the far-end osteotomy module body, clamping teeth are arranged on the clamping side of the clamping end, and the clamping teeth are matched with the sliding teeth on the sliding rod; a positioning groove is formed in the bottom of the far-end osteotomy module body, and reinforcing ribs are arranged on the two sides of the positioning groove. According to the intramedullary nail, extramedullary positioning is adopted, the application range is wider, the integrated forming technology is adopted, the cost is greatly reduced, the production period is shortened, the precision is higher, the infection risk is reduced, locking can be achieved through elastic deformation of medical-grade nylon materials, and the safety can be improved through the reinforcing rib structure adopted at the bottom.

This invention discloses a class of fluorescent compounds containing indole-diones and indole-quinoxaline derivatives, their preparation methods, and uses, belonging to the field of fluorescent compound technology. This invention provides a novel class of fluorescent compounds containing indole-diones and indole-quinoxaline derivatives with structures shown in general formulas (I) and (II). These compounds exhibit low toxicity and long emission wavelengths, showing significant application potential in the field of bioimaging.

This invention belongs to the field of biomedicine, specifically relating to a nitrogen-rich, high-strength, and tough Ti-Nb alloy, its additive manufacturing method, and its applications. The alloy comprises Ti, Nb, and N; wherein the atomic percentage of Nb is greater than 20% and less than 30%; and the mass percentage of N is 0.01 wt.% to 0.5 wt.%. The preparation method is as follows: raw material powders are mixed according to the designed composition in a nitrogen-containing protective atmosphere to obtain 3D printing powder. The powder is then printed layer by layer using a laser power of 180–220 W, a scanning speed of 300–800 mm / s, a scanning spacing of 80–120 μm, and a powder layer thickness of 25–45 μm. The scanning strategy involves an angle of 67° between each layer and the previous layer. The final product is obtained; the resulting alloy has a density of 99.8%. This invention features a simple composition system, a concise process, controllable preparation costs, and excellent product performance, making it suitable for use as orthopedic implant materials.

The application belongs to the technical field of biology, and specifically discloses a kind of composition containing fish oil and polypeptide and its use in anti-inflammation.The core active ingredient of the anti-inflammatory composition is fish oil and anti-inflammatory polypeptide P1, P2 and P3 from deep-sea fish, and the amino acid sequences are SEQ ID NO.1:QPMPANCNSTG, SEQ ID NO.2:VQWMMNHF and SEQ ID NO.3:TETSQKE.Three polypeptides are naturally safe and have no cytotoxicity, can significantly down-regulate the NO production of inflammatory cells, and strongly inhibit the secretion of core pro-inflammatory factors.The application scientifically compounds fish oil and anti-inflammatory polypeptide from deep-sea fish, and the two produce significant synergistic anti-inflammatory effect after compounding, and the anti-inflammatory effect is much better than single fish oil or single polypeptide, which can be widely applied to the preparation of anti-inflammatory drugs, anti-inflammatory health foods, anti-inflammatory functional dietary supplements and skin anti-inflammatory repair cosmetics, and has broad industrialization popularization and clinical application prospect.

The invention belongs to the technical field of dressings, and particularly relates to a gel dressing containing recombinant collagen as well as a preparation method and application of the gel dressing. The gel dressing containing the recombinant collagen is prepared from the following raw materials in percentage by weight: 0.1 to 1 percent of the recombinant collagen, 0.5 to 1 percent of sodium hyaluronate, 0.08 to 0.15 percent of radices stellariae dichotomae cyclopeptide, 0.5 to 1 percent of carbomer, 0.03 to 0.06 percent of cryptomeria fortunei bud extract, 15 to 20 percent of poloxamer, 0.1 to 0.5 percent of xanthan gum, 3 to 8 percent of glycerol, 0.04 to 0.08 percent of edetate disodium, 0.1 to 0.3 percent of triethanolamine, 0.08 to 0.12 percent of preservative and the balance of deionized water. The gel dressing has excellent biocompatibility, good hemostasis, cell growth promotion and tissue repair performance, and can be used for repairing vaginal mucous membrane chap and hemostasis of diffuse superficial bleeding, and improving the symptoms of elasticity reduction and the like caused by vaginal atrophy.

The present application relates to a kind of 3D printing titanium alloy-based ceramic crown micro-arc oxidation preparation method and product, belong to dental restoration preparation.The present application discloses a kind of 3D printing titanium alloy-based ceramic crown micro-arc oxidation preparation method and the 3D printing titanium alloy-based ceramic crown prepared, aims to solve the technical problems such as the ceramic layer of existing composite crown easy to fall off, performance is uneven, biological compatibility is insufficient, preparation process is complicated, etc., by 3D printing accurate preparation titanium alloy dental crown matrix, in situ generation even, dense, ceramic film layer with strong binding force on the surface of matrix, form the composite structure of " internal titanium alloy, external ceramic", give consideration to the high strength, toughness and biological compatibility of titanium alloy, and the aesthetic, wear resistance and corrosion resistance of ceramic, simplify preparation procedure, improve the adaptability and service life of dental crown, meet the dental clinical repair needs.

The invention discloses a jellyfish crude polypeptide with tyrosinase inhibitory activity, a jellyfish polypeptide and a preparation method and application thereof, and belongs to the technical field of biology. A jellyfish crude polypeptide is prepared, a jellyfish polypeptide GGDYLSIHMRR is identified and optimized from the jellyfish crude polypeptide, and experiments prove that both the jellyfish crude polypeptide and the jellyfish polypeptide provided by the invention can inhibit tyrosinase activity in vitro, and can inhibit melanin secretion of B16-F10 mouse melanoma cells and inhibit tyrosinase activity in the cells. Therefore, the jellyfish crude polypeptide and the jellyfish polypeptide can be used as a tyrosinase inhibitor and an anti-melanin synthesis agent and are applied to the fields of cosmetics, medical treatment and the like. Experiments prove that the jellyfish crude polypeptide and the jellyfish polypeptide provided by the invention have no cytotoxicity, are derived from oceans, and have no disease risks and religious barriers of terrestrial animals.

The present invention relates to an antibacterial and antioxidant composition containing grape callus culture solution, as an active ingredient, comprising epsilon(ε)-viniferin, delta (δ)-viniferin, resveratrol, and the like, and, specifically, grape callus, its culture solution, and its extract according to the present invention exhibit no cytotoxicity, have significant antioxidant and antibacterial effect against Propionibacterium acnes, and have remarkable antioxidant and antibacterial effect compared to pure viniferin, in particular, and thus, the grape callus, its culture solution, and its extract can be effectively used as active ingredients in antioxidant and antibacterial compositions.

The present application relates to the technical field of biotechnology, and particularly discloses a polypeptide, a recombinant humanized collagen of type XIX and application thereof. The amino acid sequence of the polypeptide comprises n repeating units; the repeating unit comprises any one of the following amino acid sequences or a variant sequence thereof: (1) an amino acid sequence shown in any one of SEQ ID NO. 1-2; (2) an amino acid sequence having at least 50%, 60%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the amino acid sequence shown in any one of SEQ ID NO. 1-2; (3) a variant sequence obtained by mutating one or more amino acid residues in the amino acid sequence shown in any one of SEQ ID NO. 1-2; wherein n is an integer greater than or equal to 1. The polypeptide has a collagen triple helix structure, has good cell adhesion activity, can perform the function of human collagen, has no immune rejection and allergic reaction, and can be used in various fields such as cartilage repair, biological dressing and tissue filling.

The invention belongs to the technical field of plant pharmacy, and particularly relates to an effect of semen momordicae in resisting respiratory syncytial virus infection. Detecting that the semen momordicae protein extract is non-toxic to HEp-2 cells through a cell proliferation detection kit; a cell viability detection method and an indirect immunofluorescence method are adopted to measure that the semen momordicae protein extract has an obvious inhibition effect on respiratory syncytial viruses, and the inhibition effect is most obvious under the concentration of 400 micrograms per milliliter. Therefore, the semen momordicae has relatively high biological safety and relatively strong effect of resisting respiratory syncytial virus infection.

The invention discloses gas-liquid-solid three-state gel soil as well as a preparation method and application thereof. The gas-liquid-solid three-state gel soil comprises a solid state, a liquid state and a gas phase which are mutually interwoven to form a three-dimensional continuous structure; the solid phase takes a hydrophilic polymer cross-linked network and hydrophobic porous particles as a solid phase skeleton, the hydrophilic polymer cross-linked network forms a three-dimensional continuous liquid phase channel through a chemical or physical cross-linking effect, and the hydrophobic porous particles are dispersed and anchored in the hydrophilic polymer cross-linked network; the liquid phase is composed of a nutrient solution; the gas phase is composed of internal pores of hydrophobic porous particles and intergranular gaps formed by mutual contact and stacking of the hydrophobic porous particles, and a three-dimensional continuous gas diffusion channel penetrating through the whole soil is formed. The gel soil with an intrinsic gas-liquid-solid three-state continuous structure is provided by constructing a continuous gas phase and continuous liquid phase double-network structure, and the problem that the water-retaining property and the air permeability in a soilless culture substrate cannot be synergistically optimized is fundamentally solved.