Preparation method of polycaprolactone tissue engineering scaffold

A technology of tissue engineering scaffold and polycaprolactone, which is applied in the field of biomedical materials, can solve the problems of surrounding tissue inflammation, difficult to remove, and cytotoxicity

Inactive Publication Date: 2017-03-15
王淑芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvents and cross-linking agents used in the prior art are difficult to remove, resulting in cytotoxicity when tissue engineering scaffold materials are used, causing inflammatory reactions in surrounding tissues, destroying the biological activity of cells and tissues, and affecting the formation of new tissues and repair effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of polycaprolactone tissue engineering scaffold is realized through the following processes:

[0026] (1) Prepare polycaprolactone (granule), polyethylene oxide (powder), and sodium bicarbonate (powder) in a volume ratio of 1:1:1 to make a mixed solution;

[0027] (2) spinning the raw material mixture in step (1) to obtain polycaprolactone nanofibers;

[0028] (3) Put the polycaprolactone nanofiber of step (2) into the tissue engineering scaffold mold, then put the tissue engineering scaffold mold with the nanofiber into the supercritical CO with a pressure of 20MPa 2 Medium treatment, control the treatment temperature at 55°C, treat for 1 hour, and then depressurize and discharge CO 2 , the control time is 1 minute, the temperature during decompression is controlled at 70 ℃, and the intermediate product is obtained. The obtained intermediate product is leached in a circulating water bath and then vacuum-dried to obtain a porosity of 68.2%. Polyca...

Embodiment 2

[0030] Prepare polycaprolactone (granular), polyethylene oxide (powder), sodium bicarbonate (powder) according to the volume ratio of 3:2:3, the same steps as in Example 1, parameters are adjusted, wherein the supercritical CO 2 The middle treatment temperature is controlled at 55°C, treated for 1 hour, and then decompressed to discharge CO 2 , the control time is 1 minute, the temperature during decompression is controlled at 70 ° C, and the internally interconnected porous material polycaprolactone tissue engineering scaffold is obtained. The porosity of the obtained porous material is 73.8%, and the cell size is 20 Micron-250 micron, the size of the internal interconnecting channel is 20 micron-200 micron.

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Abstract

The invention relates to a preparation method of a polycaprolactone tissue engineering scaffold. The method includes preparing 25-40% of polycaprolactone, 30-45% of polyoxyethylene and 25-40% of sodium bicarbonate into mixed solution, performing spinning to obtain polycaprolactone nanofibers, treating the nanofibers in supercritical CO2 to obtain an intermediate product by means of controlling treatment pressure and treatment temperature, and subjecting the obtained intermediate product to percolation in circulating water bath prior to vacuum drying to obtain the tissue engineering scaffold made of a porous material with the porosity reaching 68-74.0%, the cell size being 20-200 microns and the size of internally mutually communicated channels being about 20-200 microns. The preparation method can finish inter-fiber crosslinking and fusion at low temperature without solvent, cross-linking agent and the like, and cytotoxicity is avoided.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a polycaprolactone tissue engineering scaffold. Background technique [0002] Tissue engineering scaffold material is a material that can be combined with tissue living cells and can be implanted in organisms. It can provide cells with a place to obtain nutrients, gas exchange, excrete waste, and grow and develop. The material basis of organs. [0003] For tissue engineering scaffolds, tissue engineering scaffold materials are the key to the regeneration of tissue defects. The porosity and pore size of tissue engineering scaffold materials are important factors affecting the performance of tissue engineering scaffold materials. In addition to requiring tissue engineering scaffold materials to have a high porosity, there are also strict requirements on the size of the pores. If the pores are too small, cells cannot enter the pores...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/18
CPCA61L27/56A61L27/18A61L27/50
Inventor 王淑芳
Owner 王淑芳
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