Preparation method for preparing polylactic acid tissue engineering scaffold

A tissue engineering scaffold, polylactic acid technology, applied in the field of biomedical materials, can solve the problems affecting the formation and repair of new tissue, destroying the biological activity of cells and tissues, and the inflammatory response of surrounding tissues

Inactive Publication Date: 2017-04-19
王淑芳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The solvents and cross-linking agents used in the prior art are difficult to remove, resulting in cytotoxicity when tissue engineering scaffold materials are used, causing inflammatory reactions in surrounding tissues, destroying the biological activity of cells and tissues, and affecting the formation of new tissues and repair effect

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A preparation method of polylactic acid tissue engineering scaffold is realized through the following processes:

[0026] (1) Mix polylactic acid (granular), polyethylene oxide (powder), and sodium bicarbonate (powder) in a volume ratio of 1:1:1 to make a mixed solution;

[0027] (2) spinning the raw material mixture of step (1) to obtain polylactic acid nanofibers;

[0028] (3) Put the polylactic acid nanofibers of step (2) into the tissue engineering scaffold mold, then put the tissue engineering scaffold mold with nanofibers into the supercritical CO with a pressure of 20MPa 2 Medium treatment, control the treatment temperature at 55°C, treat for 1 hour, and then depressurize and discharge CO 2 , the control time is 1 minute, the temperature during decompression is controlled at 70 ℃, and the intermediate product is obtained. The obtained intermediate product is leached in a circulating water bath and then vacuum-dried to obtain a porosity of 68.2%. Polylactic acid...

Embodiment 2

[0030] Prepare polylactic acid (granular), polyethylene oxide (powder), and sodium bicarbonate (powder) according to the volume ratio of 3:2:3, and adjust the parameters in the same steps as in Example 1, wherein the supercritical CO 2 The middle treatment temperature is controlled at 55°C, treated for 1 hour, and then decompressed to discharge CO 2 , the control time is 1 minute, and the temperature during decompression is controlled at 70 ° C to prepare a polylactic acid tissue engineering scaffold with interconnected porous materials. The porosity of the obtained porous material is 73.8%, and the cell size is 20 μm- 250 microns, and the size of the internal interconnecting channels is 20 microns to 200 microns.

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Abstract

The invention relates to a preparation method for preparing a polylactic acid tissue engineering scaffold. According to the method, 25-40% of polylactic acid, 30-45% of polyoxymethylene and 25-40% of sodium bicarbonate are mixed up to prepare a mixed solution and then the mixed solution is subjected to spinning to obtain polylactic acid electrospun nanofibers. The nanofibers are processed in supercritical carbon dioxide. Through controlling the processing pressure and the processing temperature, an intermediate product is obtained. The obtained intermediate product is subjected to leaching in the circulating water bath and then drying in vacuum, and then the tissue engineering scaffold is obtained. The obtained tissue engineering scaffold is made of porous materials, wherein the porosity of the tissue engineering scaffold is 68 to 74.0% and the cell size of the tissue engineering scaffold is 20 to 200 microns. The dimension of the inner communicating channel of the tissue engineering scaffold is about 20 to 200 microns. According to the technical scheme of the preparation method in the invention, fibers can be cross-linked and fused at a relatively low temperature without any solvent or cross-linking agent. Meanwhile, the generation of cytotoxicity is avoided.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a preparation method of a polylactic acid tissue engineering scaffold. Background technique [0002] Tissue engineering scaffold material is a material that can be combined with tissue living cells and can be implanted in organisms. It can provide cells with a place to obtain nutrients, gas exchange, excrete waste, and grow and develop. The material basis of organs. [0003] For tissue engineering scaffolds, tissue engineering scaffold materials are the key to the regeneration of tissue defects. The porosity and pore size of tissue engineering scaffold materials are important factors affecting the performance of tissue engineering scaffold materials. In addition to requiring tissue engineering scaffold materials to have a high porosity, there are also strict requirements on the size of the pores. If the pores are too small, cells cannot enter the pores ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/18A61L27/02
CPCA61L27/56A61L27/025A61L27/18A61L27/50
Inventor 王淑芳
Owner 王淑芳
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