Composition for delivering bioactive material or protein, and use thereof

A bioactive substance and protein technology, applied in the field of delivery of bioactive substance or protein composition, can solve the problems of limited use, difficulty in maintaining protein activity, etc., and achieve the effect of simple cost, no cytotoxicity, and high delivery efficiency

Inactive Publication Date: 2017-08-18
LEMONEX +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The currently developed protein delivery system is difficult to maintain the activity of the protein, and it is difficult to apply to various proteins, so the use is limited

Method used

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  • Composition for delivering bioactive material or protein, and use thereof
  • Composition for delivering bioactive material or protein, and use thereof
  • Composition for delivering bioactive material or protein, and use thereof

Examples

Experimental program
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preparation example Construction

[0125] The preparation method of the porous silica nanoparticles may include a method that is simple and easy to synthesize in large quantities, but is not limited thereto. For example, in the case of using laboratory equipment, at least about 1 g of the porous silica nanoparticles can be produced by the production method once, but the present invention is not limited thereto.

[0126] In an embodiment of the present disclosure, the step of enlarging the pores of the porous silica nanoparticles may include treating the porous silica nanoparticles with tri(C 1-6 Alkyl)benzenes are treated to expand pores, but are not limited thereto. For example, the three (C 1-6 The alkyl)benzene may include trimethylbenzene, triethylbenzene, tripropylbenzene, tributylbenzene, tripentylbenzene, or trihexylbenzene, but is not limited thereto. For example, three (C 1-6 The step of treating alkyl)benzene to expand the pores may include treating the porous silica nanoparticles and tri(C 1-6 Al...

preparation example 1

[0150] Preparation example 1: the synthesis of the porous silica nanoparticle (mesoporous silicanoparticle, MSN) that stomata expands

[0151] The cetyltrimethylammonium bromide (CTAB) of 3.94g and the 1M sodium hydroxide (NaOH) solution of 2.28mL are dissolved in the mixed solution of methanol and water of 800g (methanol: water=0.4:0.6, w / w). Then, while vigorously stirring the obtained solution, 1.3 mL of tetramethoxysilane (TMOS) was added to the solution under atmospheric conditions. After stirring the reaction mixture for 8 hours, it was aged overnight. The obtained white precipitate was subjected to centrifugation to remove the remaining surfactant for purification, and was washed with ethanol and water five times respectively. The porous silica nanoparticles prepared above were suspended in 20 mL of ethanol, and 4 mL of hydrochloric acid was added. Subsequently, the suspension was refluxed overnight to obtain a white powder. The produced white powder was separated ...

preparation example 2

[0153] Preparation example 2: silica nanoparticles (MSNPN) modified with Ni-NTA

[0154] After suspending 100 mg of the pore-enlarged MSN prepared in said Preparation 1 in 10 mL of toluene, 1 mL of 3-aminopropyltriethoxysilane (APTES) was added for amine functionalization. The suspension was refluxed overnight, filtered with a filter, and washed 10 times with ethanol residue to obtain amine-functionalized MSN ( Figure 10a and Figure 10b of 2). 30 mg of the amine-functionalized MSN and 100 mg of 3,3'-dithiodipropionic acid bis(N-hydroxysuccinimide ester) (DTSP) in DMSO were reacted overnight at room temperature. After washing the reaction mixture 3 times with DMSO, add 10 mL of N in DMSO α , N α - Bis(carboxymethyl)-L-lysine hydrate (aminobutyl NTA, 65 mg). The reaction was carried out at room temperature for 24 hours. Then, the NTA-modified MSNs were obtained by washing with ethanol and water 10 times, respectively. In order to Ni 2+ Binding to NTA-modified MSN in 1M...

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Abstract

The present invention relates to a composition for delivering a protein, and a method for delivering a bioactive material or a protein by using the same.

Description

technical field [0001] The present disclosure relates to a composition for delivering biologically active substances or proteins and uses thereof. Background technique [0002] The drug delivery system refers to the medical technology that minimizes the side effects of conventional drugs and maximizes the potency and effect to effectively deliver the required amount of drugs, such as proteins, nucleic acids, or other low molecules. Recently, said technology that reduces the cost and time required to develop new drugs is combined with nanotechnology to become a field of cutting-edge technology that creates new added value in the medical world, while technologically advanced countries such as the United States and Japan have changed from the past Since the latter half of the 1980s, companies such as pharmaceutical companies have been making every effort to develop new drugs and drug delivery systems. [0003] So far, viral genes, recombinant proteins, liposomes, cationic poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/02A61K47/40A61K39/395
CPCA61K39/395A61K9/143C07K2317/94C07K16/00A61K47/6929A61K47/6923B82Y5/00A61K47/02A61K47/40A61K9/16A61K9/145
Inventor 元哲喜
Owner LEMONEX
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