Preparation method for abelmoschus esculentus proteoglycan protein xerium

A technology for polysaccharide protein and dry powder, which is applied in the field of preparation of okra polysaccharide protein dry powder, can solve the problems of few functional foods, uneven quality and other problems, and achieves the effects of reducing extraction cost, increasing yield and reducing enzyme price

Inactive Publication Date: 2012-07-25
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few types of functional foods for improving gastrointestinal dysfunct

Method used

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  • Preparation method for abelmoschus esculentus proteoglycan protein xerium

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Take 1000g of fresh okra fruit, add 10-20L of water, pulverize with a tissue grinder, add a compound enzyme for enzymatic hydrolysis and extraction (the compound enzyme is composed of cellulase, helicase, and lysozyme, and the mass ratio of the three is 6:2:1), The amount of enzyme added is 0.5wt%-2.5wt% of the dry weight of the okra. The enzymolysis extraction conditions are as follows: pH=6.5-7.5, temperature 32-40°C, power 600-800W, ultrasonic working time 2-5s, intermittent time 1-3s, extraction time 6-7h. After the extraction is completed, the large particle residue is removed by centrifugation, and then suction-filtered to obtain the okra polysaccharide protein extract.

[0051] Add maltodextrin to the okra polysaccharide protein extract prepared above, the amount of maltodextrin added is 1 to 35% by weight of the dry matter of the okra polysaccharide protein extract, and after the maltodextrin is added, carry out high-pressure homogenization, and the homogenizati...

Embodiment 2

[0053] Take 1000g of fresh okra fruit, add 15-20L of water, grind it with a tissue grinder, add compound enzyme to extract (the compound enzyme is composed of cellulase, helicase, and lysozyme, and the mass ratio of the three is 6.5:2.3:1), add enzyme The amount is 0.5wt% to 3wt% of the dry weight of the okra. The enzymolysis extraction conditions are as follows: pH=6.5-7.2, temperature 30-38°C, power 650-800W, ultrasonic working time 2-4s, intermittent time 1-2s, extraction time 7-8h. After the extraction is completed, the large particle residue is removed by centrifugation, and then suction-filtered to obtain the okra polysaccharide protein extract.

[0054] Add maltodextrin to the okra polysaccharide protein extract obtained above, the amount of maltodextrin added is 1 to 45 wt% of the dry matter of the okra polysaccharide protein extract, and after the maltodextrin is added, carry out high-pressure homogenization, and the homogenization pressure is 20-45 wt%. 25MP, homoge...

Embodiment 3

[0056]Take 1000g of quick-frozen fresh okra fruit, add 15-25L of water, pulverize with a tissue grinder, and extract with a compound enzyme (the compound enzyme is composed of cellulase, helicase, and lysozyme, and the mass ratio of the three is 6.3:2.2:1), add The enzyme amount is 1.0wt%-2.3wt% of the dry weight of the okra. The enzymolysis extraction conditions are as follows: pH=6.8-7.5, temperature 32-39°C, power 600-780W, ultrasonic working time 3-5s, intermittent time 2-3s, extraction time 6.5-8h. After the extraction is completed, the large particle residue is removed by centrifugation, and then suction-filtered to obtain the okra polysaccharide protein extract.

[0057] Add maltodextrin to the okra polysaccharide protein extract obtained above, the amount of maltodextrin added is 1-40wt% of the dry matter of the okra polysaccharide extract, after the maltodextrin is added, perform high-pressure homogenization, and the homogenization pressure is 16-20MP , homogeneous c...

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Abstract

The invention discloses a preparation method for abelmoschus esculentus proteoglycan protein xerium, which includes the steps: 1) adding water into abelmoschus esculentus raw materials, crushing the abelmoschus esculentus raw materials with a tissue destructor, adding compound enzyme for enzymolysis and extraction prior to centrifugally removing large residue particles, and performing suction filtration to obtain abelmoschus esculentus proteoglycan protein extracting solution; and 2) adding maltodextrin into the prepared abelmoschus esculentus proteoglycan protein extracting solution, and obtaining the target product, namely the abelmoschus esculentus proteoglycan protein xerium by means of high-pressure homogenization and spray drying. As tested, essential components of the abelmoschus esculentus proteoglycan protein xerium refer to soluble polysaccharide and protein, wherein the content of the polysaccharide is >=75wt%, the content of the protein is >=10wt%, and the moisture content is >=4.2wt%. The abelmoschus esculentus proteoglycan protein xerium can effectively improve the human gastrointestinal function, and is high in active component and fine in physical characteristic, and can serve as raw materials to fill capsules or be tableted so as to produce functional food.

Description

technical field [0001] The invention belongs to the technical field of functional food production, and in particular relates to a preparation method of dry okra polysaccharide protein powder. Background technique [0002] Okra [Abelmoschus esculentus (L.) Moench], alias okra, okra, also known as okra abroad, is a species of Malvaceae (Abelmoschus) okra (Malvaceae), an annual herb, widely distributed in tropical to subtropical region. Okra has multiple names in my country, also known as coffee ambrette, croissant, foreign sesame, eggplant, kidney-tonifying grass, pearl vegetable, etc. According to "Compendium of Materia Medica" and other ancient books, the roots, stems, flowers, and seeds of okra can be used as medicine. Its nature and flavor are sweet, cold, and slippery. Intestinal dryness, constipation, malignant sores, carbuncles and other diseases. [0003] At present, okra is cultivated more in countries and regions such as the United States, Japan, Southeast Asia, E...

Claims

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Application Information

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IPC IPC(8): C08L5/00C08L89/00C08L3/02C08B37/00C07K14/435C07K1/14G01N5/04
Inventor 祝子坪唐雪明王金斌
Owner SHANGHAI ACAD OF AGRI SCI
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