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Preparation method and special culture solution for artificial seeds of broussonetia papyifera

A technology of artificial seeds and culture fluid, applied in botany equipment and methods, horticultural methods, applications, etc., can solve the problems of large site occupation, low efficiency, and high labor costs

Active Publication Date: 2016-06-22
大连中植环境生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cuttings have problems such as seasonality, large site occupation, low efficiency, and high labor intensity.
Conventional tissue culture methods have problems such as high labor costs, heavy labor, and high costs

Method used

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  • Preparation method and special culture solution for artificial seeds of broussonetia papyifera
  • Preparation method and special culture solution for artificial seeds of broussonetia papyifera

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, preparation somatic embryo

[0068] 1. Preparation of embryogenic callus

[0069] (1) Get the leaves of the aseptic seedlings of the tree, cut into 0.5 × 0.5cm 2 , inoculated on Medium A, and cultivated in the dark at 20-25°C for 25 days. At this time, it can be observed that light yellow callus grows from the leaf cut, that is, embryogenic callus.

[0070] (2) Take the embryogenic callus tissue obtained in step (1), inoculate it on medium A, and culture it in the dark at 20-25° C. for 25 days.

[0071] (3) Take the embryogenic callus obtained in step (2), inoculate it on medium A, and culture it in the dark at 20-25° C. for 25 days.

[0072] In actual operation, the subculture can be continuously carried out according to the method of step (2).

[0073] Medium A (solid): take MS liquid medium, add 0.5mg 2,4-dichlorophenoxyacetic acid, 30g sucrose and 5g agar powder per liter.

[0074] 2. Liquid suspension culture

[0075] (1) Add 1 g of the embryoge...

Embodiment 2

[0085] Embodiment 2, according to the method provided by the present invention prepares the artificial seed of mulberry tree

[0086] Preparation method of culture medium (pH6.0): take 1 / 10 MS medium, add 2mg6-BA, 1mgNAA, 20g sucrose, 1g low molecular weight chitosan, 1g oxalic acid and 40g sodium alginate per liter.

[0087] 1. Put 1.5×10 4 One (about 10 g) of the somatic embryos obtained in Example 1 was put into 1500 mL of culture medium D, mixed evenly, and incubated at room temperature for 3 minutes.

[0088] 2. Take the system obtained in step 1 with a rubber dropper and add it dropwise to the 2 Incubate at room temperature for 30 minutes in a beaker of aqueous solution, discard the supernatant, and rinse with sterile deionized water for 3-4 times to obtain artificial seeds of mulberry.

Embodiment 3

[0089] Embodiment 3, according to existing method preparation tree artificial seed

[0090] The preparation method of culture solution E: take 1 / 2 MS medium, add 30g of sodium alginate per liter.

[0091] Put the somatic embryo obtained in Example 1 into the culture solution E and soak at room temperature for 3-4min, then transfer to 0.5g / 100mL CaCl 2 soak in an aqueous solution at room temperature for 3 minutes, discard the supernatant, and rinse with sterile deionized water for 3-4 times to obtain the artificial seeds of the mulberry tree.

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Abstract

The invention discloses a preparation method and a special culture solution for artificial seeds of broussonetia papyifera. The invention firstly provides the preparation method for the artificial seeds of the broussonetia papyifera. The preparation method comprises the following steps of: (a1) incubating somatic embryos of the broussonetia papyifera in a culture solution IV, wherein the culture solution IV is prepared by adding 1.8-2.2mg of 6-BA, 0.9-1.1mg of NAA, 18-22g of saccharose, 0.9-1.1g of lower-molecular weight hitosan, 0.9-1.1g of oxalic acid and 36-44g of sodium alginate into per litre of 1 / 10MS culture medium; and (a2) adding a system obtained in the step (a1) into a 0.9-1.1g / 100mL CaCl2 aqueous solution, and incubating to obtain the artificial seeds of the broussonetia papyifera. The invention further provides a culture solution for preparing the artificial seeds of the broussonetia papyifera, wherein the culture solution is the culture solution IV. The preparation method and the special culture solution are capable of realizing efficient propagation of broussonetia papyifera seedlings and meeting the market requirement of the broussonetia papyifera seedlings, and have great promotional value.

Description

technical field [0001] The invention relates to a method for preparing mulberry artificial seeds and a special culture solution thereof. Background technique [0002] Broussoneti Papyrifera (L) Vent, also known as mulberry tree, is a deciduous tree belonging to Moraceae. It can grow in almost all types of soil, has few diseases and insect pests, is resistant to dry and cold, damp and hot, and has developed root system. A well-maintained tree species. The crown of the mulberry tree is relatively large, and it has a strong ability to absorb automobile exhaust and other types of haze dust in the air. Therefore, it is also a commonly used tree species for environmental greening and street trees. The whole body of the mulberry tree can be used: the bark fiber is white and slender, and it is the raw material and textile raw material for the manufacture of high-grade paper such as coated paper. The crude protein, amino acid, and trace elements are the raw materials for preparing ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/006
Inventor 张宗申杨雨迎陈阳刘兴斌李毅熊伟
Owner 大连中植环境生物科技有限公司
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