Preparation method of modified lactobacillus acidophilus peptidoglycan
A technology of Lactobacillus acidophilus and peptidoglycan, which is applied in the field of preparation of modified Lactobacillus acidophilus peptidoglycan, can solve the problem of unfavorable PG efficacy research and industrialization, large changes in PG molecular structure, difficult control of enzyme activity, etc. problem, to achieve the effect of improved solubility and antioxidant activity, short reaction time, and improved solubility
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0024] A preparation method of modified Lactobacillus acidophilus peptidoglycan, comprising the following steps:
[0025] (1) Preparation of wet cells
[0026] Put 3g of Lactobacillus acidophilus in a Erlenmeyer flask, heat and kill the bacteria in a water bath at 65°C for 40min, then add 10mL of 0.5% Triton X-100 (polyethylene glycol octylphenyl ether) solution, and bathe in a water bath at 85°C for 1h , and continuously stirred, take out the solution and put it in an ice bath to cool immediately, centrifuge at 12000×g for 10 min, discard the supernatant, wash the precipitate twice with double distilled water, and centrifuge again at 12000×g for 8-12 min Discard the supernatant, then wash with methanol-water (v:v=2:1) and methanol in sequence to remove impurities in the precipitate to obtain wet cells;
[0027] (2) Enzymatic hydrolysis and wall breaking
[0028] Add the wet bacteria to 8mL enzyme solution Ⅰ, put it in a water bath at 37°C for 12 hours, then centrif...
Embodiment 2
[0037] With embodiment 1, its difference is:
[0038] In step (1): put Lactobacillus acidophilus in the Erlenmeyer flask, bathe in water at 60°C for 45 minutes, then add 0.5% Triton X-100 solution, bathe in water at 80°C for 1.5h, and keep stirring continuously, take out the solution and put it in an ice bath Cool immediately, centrifuge at 10,000×g for 12 min, discard the supernatant, wash the precipitate twice with double distilled water, centrifuge again at 10,000×g for 12 min, and discard the supernatant.
[0039] In step (2): add wet bacteria to enzyme solution Ⅰ, place in 35°C water bath for 14 h, then centrifuge at 4°C, 10,000×g for 50 min, discard the supernatant, take the precipitate and add to enzyme solution Ⅱ, place in 35°C water bath for 14 h h, then centrifuge at 4°C, 10,000×g for 50 minutes, discard the supernatant, take the precipitate and add it to enzyme solution III, treat it in a water bath at 35°C for 20 minutes, then centrifuge at 4°C, 10,000×g for 50 m...
Embodiment 3
[0044] With embodiment 1, its difference is:
[0045] In step (1): put Lactobacillus acidophilus in the Erlenmeyer flask, bathe in water at 70°C for 35 minutes, then add 0.5% Triton X-100 solution, bathe in water at 90°C for 0.5h, and keep stirring continuously, take out the solution and put it in an ice bath Cool immediately, centrifuge at 15,000×g for 8 min, discard the supernatant, wash the precipitate twice with double distilled water, and centrifuge again at 15,000×g for 8 min, then discard the supernatant.
[0046] In step (2): add wet bacteria to enzyme solution Ⅰ, bathe in water at 40°C for 10 hours, then centrifuge at 4°C and 15,000×g for 30 minutes, discard the supernatant, take the precipitate and add it to enzyme solution Ⅱ, bathe in water at 40°C for 10 hours, Then centrifuge at 4°C, 15,000×g for 30 minutes, discard the supernatant, take the precipitate and add it to enzyme solution III, treat it in a water bath at 40°C for 20 minutes, then centrifuge at 4°C, 15...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap