Method for extracting alkaloid, polysaccharide and flavone from mulberry leaves
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A technology of alkaloids and mulberry leaves, applied in the field of extraction and purification of natural products, can solve the problems of wasting materials, insufficient research, increasing costs, etc., and achieves the effects of convenient operation, low cost and cost saving
Inactive Publication Date: 2015-09-30
TARIM UNIV
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[0004] There are many domestic and foreign research reports on the pharmacological activity of the chemical components of Morus, but the research on the characteristic species of Xinjiang—Mulberry mulberry is not enough. In recent years, relevant research on the biologically active components and pharmacological effects of Mulberry mulberry has been carried out in China. And some progress has been made: Xu Li’s research group from Southwest University obtained morin from the medicinal mulberries by soaking in ethanol, organic solvent extraction and resin purification; Zhou Jing analyzed the nutritional components of three kinds of mulberry in Kuqa, Xinjiang, and obtained the medicinal mulberry It has the highest nutritional value; Maimaiti Yiming reported the separation of flavonoids and polyphenols from the medicine mulberry; the extraction of the polysaccharides from the medicine mulberry was also reported; Song analyzed 33 different mulberry leaves in my country, including Xinjiang medicine mulberry and Hetian white mulberry. The content of several active substances such as NDJ, flavonoids, resveratrol, etc., most studies mainly focus on the extraction and content determination of a single target chemical component, but alkaloids, polysaccharides and flavonoids cannot be obtained at the same time, which wastes materials and increases cost
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Embodiment 1
[0050] Embodiment 1, extract alkaloid, polysaccharide and flavonoid from mulberry leaf
[0051] as per figure 1 The flow chart shown is for the extraction of alkaloids, polysaccharides and flavonoids in mulberry leaves.
[0052] (1) Extraction:
[0053] 1) Take 100 mesh medicinal mulberry leaf powder of 100g in a 5000ml round bottom flask, add hydrochloric acid / ethanol aqueous solution and heat to reflux, wherein the concentration of hydrochloric acid is 0.02mol / ml, the mass percentage concentration of ethanol is 60%, the The mass-to-volume ratio (that is, the mass ratio of the mixture of medicinal mulberry leaf powder to hydrochloric acid and ethanol solution) is 20g / L, the extraction temperature is 80°C, the extraction time is 60min, and the extraction times are 3 times. Filtration to obtain the extract I (that is, the crude extraction product containing alkaloids and polysaccharides) and filter residue;
[0054] 2) Re-add the filter residue in 1) into the aqueous solutio...
Embodiment 2
[0069] Implementation 2. Condition optimization for extracting alkaloids, polysaccharides and flavonoids from mulberry leaves
[0070] The same method as in Example 1, as shown in Table 1, different conditions correspond to the contents of extracted alkaloids, polysaccharides and flavonoids. figure 2 , 3 Shown in and 4 are the interaction diagrams of the conditions for extracting alkaloids, polysaccharides and flavonoids in the present invention respectively.
[0071] Table 1 Response values of extraction volumes corresponding to different extraction conditions
[0072]
[0073] Using Design-Expert V8.0.6.1 software for multiple regression fitting, the regression model equation was obtained:
[0074] Y 1 =-19.39+0.43X 1 +0.56X 2 +0.04X 3 +122.99X 4 -3.75×10 -6 x 1 x 2 +6.56×10 -5 x 1 x 3 +0.17X 1 x 4 +3.38×10 -5 x 2 x 3 -0.94X 2 x 4 +0.06X 3 x 4 -3.71×10 -3 x 1 2 -0.01X 2 2 -3.53×10 -4 x 3 2 -2893.85X 4 2
[0075] R 2 =0.9306
[0076] Y...
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Abstract
The invention provides a method for extracting alkaloid, polysaccharide and flavone from mulberry leaves. The method comprises the following steps that mulberry leaf powder is extracted in an acid solution, and an extracting solution I and a filter residue are collected; the filter residue is extracted in an alkaline solution, and an extracting solution II is collected; the extracting solution I and the extracting solution II are treated, deposits are separated, an extracting solution III and an extracting solution IV are collected respectively, solvents are removed, and aqueous solutions are prepared respectively; the aqueous solution of the extracting solution III is adsorbed by cation exchange resin, the adsorbed cation exchange resin is eluted by water and alkaline liquid sequentially, a water phase I and an alkaline liquid phase are collected respectively, a solvent is removed from the alkaline liquid phase, and the alkaloid is obtained; the aqueous solution of the extracting solution IV is adsorbed by macroporous resin, the adsorbed macroporous resin is eluted by water and an organic solvent sequentially, a water phase II and an organic phase are collected respectively, a solvent is removed from the organic phase, and the flavone is obtained; water is removed from the deposits, the water phase I and the water phase II, and the polysaccharide is obtained. The method can save raw materials, and can extract the alkaloid, the polysaccharide and the flavone from the same batch of mulberry leaf powder.
Description
technical field [0001] The invention relates to a method for extracting alkaloids, polysaccharides and flavonoids from mulberry leaves, belonging to the technical field of extraction and purification of natural products. Background technique [0002] Mulberry leaves, also known as iron fan, are the leaves of the mulberry tree of the moraceae family (moraceae) and are a medicinal and edible plant leaf issued by the Ministry of Health. It tastes sweet, bitter, and cold in nature. , returns lung, Liver Meridian, has the effects such as dispelling wind-heat, calming liver-yang, clearing lung-heat and moistening dryness, hemostasis and cooling blood. There are about 16 species of Morus (Morus, Moraceac) in the world, distributed in the northern temperate zone, tropical Asia, tropical Africa and the Americas. China is famous for its rich mulberry resources, and its annual output of mulberry leaves ranks among the top in the world. Mulberry leaves are distributed all over the coun...
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