Helicobacter pylori (HP) type and drug-resistant mutation gene detection kit

Abstract

The invention relates to a gene detection kit, in particular to a helicobacter pylori (HP) type and drug-resistant mutation gene detection kit. The kit comprises PCR (polymerase chain reaction) solutions and nucleic acid membrane strips for HP type and drug-resistant mutation gene detection; the PCR solutions comprise a PCR solution I, a PCR solution II, a PCR solution III and a PCR solution IV; the PCR solutions also contain a pair of internal control primers respectively. The HP type and drug-resistant mutation gene detection kit can be used for distinguishing two types of HP in one test, and can be used for detecting 15 mutation types of 9 hot spot mutational loci related with drug fastness of 5 therapeutics, a VacA gene and a CagA gene are used for typing, and a reference basis is provided for judgment of illnesses. Mutation types of drug-resistant mutation detection are more and more comprehensive, and the condition of genotypic resistance of HP from which a patient suffers is quickly and comprehensively evaluated.

Description

technical field [0001] The invention relates to a gene detection kit, in particular to a Helicobacter pylori typing and drug resistance mutation gene detection kit. Background technique [0002] Helicobacter Pylori (HP) was first isolated from the gastric mucosa of gastritis patients in 1983 by Barry J. Marshall and J. Robin Warren. Studies have shown that inflammation caused by HP can lead to atrophic gastritis, intestinal metaplasia, dysplasia, and eventually gastric adenocarcinoma. [0003] The whole gene sequence of HP has been determined, and the urease gene has four open reading frames, which are UreA, UreB, UreC and UreD. The polypeptides encoded by UreA and UreB are structurally equivalent to the two subunits of the urease structure. The urease of Helicobacter pylori is extremely rich, containing about 15% of bacterial protein, and its activity is 400 times that of Proteus. Urease catalyzes the hydrolysis of urea to form an "ammonia cloud" to protect bacteria from...

AI Technical Summary

Problems solved by technology

Due to the limitations of fluorescent reporter groups and instrument detection channels, the mutation types that can be detected by one PCR mix are limited, and multiple PCR mixes are required to meet the detection of so many mutation types, which is costly

Currently there is no product and method for comprehensive and rapid genotyping of Helicobacter pylori and detection of drug-resistant mutations

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