Soil DNA sample preparation kit and preparation method thereof

A technology for sample preparation and kit, applied in the field of DNA analysis, can solve the problems of complex preparation process, high difficulty, time-consuming and labor-intensive, etc., and achieve the effects of easy use, saving time and cost, and stable results.

Inactive Publication Date: 2008-08-06
SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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Problems solved by technology

[0003] The purpose of the present invention is to provide a soil DNA sample preparation kit and its preparation method to overcome the complex, difficult, time-consuming and labor-intensive problems of the existing DNA sample preparation process

Method used

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  • Soil DNA sample preparation kit and preparation method thereof
  • Soil DNA sample preparation kit and preparation method thereof

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Embodiment Construction

[0021] The technical characteristics of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0022] 1. Weigh 0.5g of the dry and dispersed soil sample to be treated (if the water content is high, centrifuge at 10,000g for 2 minutes, and take the sediment for later use) into the centrifuge tube F, add 1ml of solution I(A), and vortex slightly to mix. Centrifuge at 2000g for 1-2min, remove the supernatant, and repeat the operation once;

[0023] 2. Take 800ul of solution II(B) (note: if solution II solidifies, please place it in a 60°C water bath and heat it to dissolve) into centrifuge tube F, vortex vigorously for 2-5 minutes, and centrifuge at 10000g for 1-2 minutes;

[0024] 3. Pipette 400ul of the supernatant into a clean and sterile 1.5ml centrifuge tube (prepared by yourself), add 400ul of solution III (C), incubate at 4°C for 20 minutes, and centrifuge at 10,000g for 5-10 minutes;

[0025] 4. Asp...

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Abstract

The invention discloses a soil DNA sample preparation reagent box and a manufacturing method, the reagent box comprises a box body and a baffle board, a reagent bottle A which is filled with solution I, a reagent bottle B which is filled with solution II, a reagent bottle C which is filled with solution III, a reagent bottle D which is filled with solution IV, a reagent bottle E which is filled with suspension liquid, a centrifuge tube F which is filled with acid cleaning glass bead, a mini filter G and a PCR(chain type polyase reaction) pipe H. A reagent box which is designed through the technical proposal of the invention has the advantages of simple employment and stable result, which can effectively save time and cost of users.

Description

technical field [0001] The invention relates to a soil DNA sample preparation kit and a preparation method thereof, which are used for the preparation of DNA samples for analyzing the diversity of soil bacteria by denaturing gradient gel electrophoresis, and belong to the technical field of DNA analysis. Background technique [0002] At present, denaturing gradient gel electrophoresis is the most widely used method for analyzing the diversity of soil bacteria. The DNA sample preparation process for denaturing gradient gel electrophoresis includes the extraction and purification of total soil DNA and the amplification of target DNA. However, the soil DNA extraction and purification process is complicated, the methods are diverse, the amplification is difficult, the preparation process is time-consuming and laborious, and the results are not comparable. Contents of the invention [0003] The object of the present invention is to provide a soil DNA sample preparation kit and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/68
Inventor 赵庆节沈根祥梁丹涛胡双庆郭春霞顾海蓉钱晓雍黄丽华周海花
Owner SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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