Preparation method of composite film for preventing postoperative adhesion

Abstract

The invention relates to a preparation method of a composite film for preventing postoperative adhesion, and belongs to the technical field of films. The preparation method comprises the following steps: (1) mixing polysaccharide with normal saline to obtain a 1.5wt%-4wt% polysaccharide solution, mixing with 8%-12% (V / V) acetic acid, and standing to obtain a solution I; (2) mixing hyaluronic acid with the normal saline to obtain a 0.3wt%-0.5wt% hyaluronic acid solution, and standing to obtain a solution II; (3) mixing hydroxyethyl starch with normal saline to obtain a 0.1wt%-4wt% hydroxyethyl starch solution III; and (4) mixing the solution I, the solution II and the solution III at the volume ratio of 1 to 1 to 1, adding glycerinum to obtain a 2.5wt%-5wt% solution IV, stirring, carrying out ultrasonic treatment, standing, spreading and drying to obtain the composite film. The prepared composite film has the beneficial effects that the composite film can stably cover the surface of a wound in a long-acting manner; and the target of preventing adhesion to other organs or tissues is reached through a physical barrier.

Description

technical field [0001] The invention relates to a preparation method of a composite membrane for preventing postoperative adhesion, and belongs to the technical field of membranes. Background technique [0002] Tissue adhesion (adhesion) is a wound caused by inflammation, friction, surgery, etc. During the treatment process, excessive fibrous tissue is produced or blood clots are formed by bleeding, which causes adhesion of surrounding organs and tissues. This phenomenon is very easy to occur after surgical operations such as the human abdominal cavity, cardiovascular, spine, bone joints, and gynecological pelvic cavity. Among them, the incidence of abdominal and pelvic adhesions is as high as 90%, about 20% of female infertility patients are caused by pelvic adhesions, and 30% of the adhesions have obvious symptoms, such as chronic pelvic pain, nausea and flatulence caused by intestinal adhesions and constipation etc. Tissue adhesion not only brings great pain to patients...

AI Technical Summary

Problems solved by technology

However, because it is easily decomposed and absorbed in the body, the anti-tissue adhesion effect is limited

In order to improve the easy decomposition of hyaluronic acid in the body, the U.S. Patent (Patent No. 6,387,413B1) adopted the method of grafting hyaluronic acid with a polymer compound (Carboxymethyl cellulose, CMC) to delay its decomposition speed in the body. Although the cellulose in the anti-adhesion film is a natural polymer material, because it is not a substance in the body, when it is injected into the body, it will react with some substances in the body, causing adverse reactions. In addition, there is no enzyme in the body to decompose it. It can be used in the body after hydrolysis and oxidation treatment

U.S. Patent (Patent No. 5,017,229) disclosed the use of hyaluronic acid to prepare a hydrophobic anti-adhesive film through EDC [1-ethyl-3 (3-dimethylaminopropyl) carbodilimide hydrochloride] grafted CMC (Carboxymethyl cellulose) polymer material Technology, the grafting method is formed by the combination of positive and negative charges between compounds. The anti-adhesion film is not easy to decompose and has anti-adhesion effect, but the EDC chemical substance is toxic to the human body, and it takes a long time for special treatment to remove it

[0004] In summary, although the currently developed anti-adhesion materials have anti-adhesion properties, it is difficult to remove the anti-adhesion materials prepared by chemical methods to promote the synthesis of chemical additives, so their toxicity and safety issues have restricted their wide clinical application. the bottleneck

Most of the anti-adhesion agents currently used in the market are hydrogels, which require a large amount of clinical use and high cost; at the same time, due to external factors such as the environment, the active ingredients cannot be uniformly and specifically act on the wound site

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