12 results about "Intestinal adhesions" patented technology

The invention discloses lactobacillus mucus YF28 and application thereof, and belongs to the technical field of microorganisms. The fermented lactobacillus mucus YF28 is separated from Hu sheep manure, is classified and named as the fermented lactobacillus mucus YF28, and has the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 20252433. The fermented lactobacillus mucilaginosus YF28 has good tolerance in the gastrointestinal environment and can reach the intestinal tract to play a role. The bacterial strain has strong intestinal adhesion capacity, is easy to form a probiotic biological membrane, and is beneficial to intestinal health. The fermented lactobacillus mucus YF28 can promote peristalsis, enrich the abundance of Akkermansia bacteria and bacteriophage thereof, restore the water content of excrement to a normal level, and significantly promote intestinal health. The invention has an application prospect in the development of drugs or health-care foods with the function of relieving constipation and related gastrointestinal symptoms.

This invention provides a *Lactobacillus plantarum* LN-Lp1 strain, its applications, products, and methods, belonging to the field of probiotic technology. The *Lactobacillus plantarum* LN-Lp1 strain has the accession number CGMCC 33158. This LN-Lp1 strain was deposited on December 23, 2024, at the China General Microbiological Culture Collection Center, located at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing. Based on this strain, this invention also provides its uses and related products. This LN-Lp1 strain exhibits strong resistance to simulated gastric-intestinal stress and good intestinal adhesion and colonization potential. The *Lactobacillus plantarum* LN-Lp1 of this invention has excellent weight-loss effects, improving lipid metabolism and lowering blood lipid levels.

The present disclosure can improve the degree of soy protein proteolysis and the ability to produce valine, isoleucine, and leucine, which are branched-chain amino acids, as well as other amino acids, such as threonine, glycine, tyrosine, and lysine. Accordingly, the present disclosure can prevent sarcopenia and has antioxidant activity. Also, the present disclosure can maintain protein metabolic balance and relieve sarcopenia by muscle synthesis.The self-aggregation, hydrophobicity, and intestinal adhesion of a mixed strain is improved compared to a case where a single strain used for the mixed strain is used. Therefore, when the mixed strain of the present disclosure is ingested, the strain can remain for a long time in the intestine.

The application discloses a kind of synergistic lycium barbarum polysaccharide-glycoside composite nutritional supplement and preparation method thereof, belong to food nutritional supplement preparation technical field.New fresh Ningxia wolfberry is used as raw material, high-activity lycium barbarum polysaccharide is obtained by enzyme hydrolysis-ultrasonic synergistic extraction, fractional ultrafiltration purification, and target group and crosslinking site are introduced by "click chemistry" strategy;Ginkgo biloba flavonoid glycoside, astragalus saponin and gardenia jasminoides glycoside with specific active conformation are selected, and after being directionally modified by enzyme method, site-specific coupling is carried out with modified lycium barbarum polysaccharide, forming "polysaccharide-glycoside" target coupling compound;After compounding intestinal adhesion type adjuvant and antioxidant protective agent, the finished product is prepared after low-temperature vacuum spray granulation and target modification.The application realizes the double breakthrough of targeted delivery and synergistic effect, the intestinal target enrichment rate is increased by more than 60%, the bioavailability of active ingredient is 90%, the activity retention rate is more than 98% after 6 months of storage, and the antioxidant and immunomodulatory functions are increased by about 2 times compared with traditional products.

The application discloses sulfhydrylated oxidized guar gum / sodium alginate microspheres and a preparation method and application thereof, and belongs to the field of biomedical materials. The sulfhydrylated oxidized guar gum and a sodium alginate base material are used to prepare and construct microspheres with pH response, colon targeting and intestinal adhesion by an emulsion gel method, the microsphere technology is used to embed probiotics, and the survival rate of the probiotics in gastric juice is improved. Through in-vitro verification, the method of embedding the microspheres successfully prevents the direct contact of the probiotics with a harsh environment, improves the ability of the probiotics to resist strong acid in the gastrointestinal tract, and enhances the adhesion and colonization effect of the probiotics in the intestinal tract. Through an animal experiment of simulating colitis, the LGG microspheres have the best treatment effect on colitis. Compared with a large number of side effects caused by antibiotic therapy, the treatment method has a certain clinical application prospect as a means with biological safety to treat inflammatory bowel disease.

The application discloses an EcN@BEVs probiotic compound and a preparation method and application thereof, and belongs to the technical field of biotechnology. The EcN@BEVs probiotic compound comprises Escherichia coli Nissle 1917 EcN and bletilla striata extracellular vesicles BEVs attached to the outer surface of the Escherichia coli Nissle 1917 EcN. The application constructs an advanced targeted delivery system for probiotic preparations, successfully connects the EcN and the BEVs in a directional manner, and constructs an engineered probiotic compound EcN@BEVs. The nano-coated BEVs attached to the outer surface of the Escherichia coli Nissle 1917 EcN serve as a rigid barrier, enhances the resistance to gastric acid and bile salts, promotes long-term retention in the intestinal tract by improving adhesion to mucosal epithelium, and improves the survival rate of probiotics under various extreme conditions in the digestive tract, and effectively promotes the colonization ability of the probiotics in the intestinal tract. Due to the anti-inflammatory properties of the bletilla striata-derived nanoparticles and the high survival rate and strong intestinal adhesion ability of the encapsulated probiotics, the EcN@BEVs probiotic compound has a better treatment effect in treating intestinal inflammation than the uncoated EcN.

The invention belongs to the technical field of food microorganisms, and particularly relates to a high-activity probiotic microcapsule based on a metal-phenolic aldehyde network composite wall material as well as a preparation method and application of the high-activity probiotic microcapsule. The microcapsule prepared by the invention takes lactic acid bacteria and the like as a core material, and the core material is compounded with a fructo-oligosaccharide-mannitol composite protective agent; a five-layer structure of sodium alginate, chitosan, EGCG, a metal ion composite layer and konjac glucomannan is innovatively constructed for a wall material, a dense metal-phenolic network (MPN) is formed through coordination of EGCG and Ca < 2 + > / Fe < 3 + > / Zn < 2 + >, and a low-temperature pre-freezing-low-temperature electrostatic spray drying synergistic process is combined to prepare the composite material. The particle size of the obtained microcapsule is 120-160 microns, and the microcapsule is resistant to gastric acid and bile salt and high in survival rate, has the characteristics of oxidation resistance and intestinal adhesion enhancement, and can be widely applied to the fields of foods, functional fermented foods, health foods, medicines and the like.

The application discloses an application of a lysine-porphyrin conjugate LD4 in preparation of a photodynamic medicine for preventing or treating tissue adhesion, and belongs to the technical field of medical anti-adhesion medicines. The medicine is administered by local smearing or spraying on a surgical wound surface, and single photodynamic treatment is carried out under the condition of a wavelength of 650-660 nm, a power density of 20-50 mW / cm2 and a light irradiation dose of 10-60 J / cm2, so that active oxygen is generated, and the anti-adhesion effect is achieved by inhibiting the activation of fibroblasts into myofibroblasts, reducing the excessive synthesis and deposition of type I collagen and other mechanisms. Experiment proves that the application has a significant prevention effect on various postoperative tissue adhesions such as abdominal wall adhesion, intestinal adhesion and tendon adhesion, the adhesion score is reduced by more than 70%, the operation is simple, the safety is high, there is no obvious toxic and side effects, and the application has a wide clinical application prospect.

This invention relates to the field of probiotic development and utilization technology, specifically to a probiotic strain of *Lactobacillus plantarum* SWUN3927 and its applications. The *Lactobacillus plantarum* (… Lactobacillus plants SWUN3927, isolated from colostrum of Tibetan yaks, was used in this invention to identify the probiotic effects of *Lactobacillus plantarum* SWUN3927 through animal experiments. The results showed that *Lactobacillus plantarum* SWUN3927 is resistant to acid and bile salts and has an inhibitory effect on pathogenic bacteria; its hydrophobicity is 74.67% and its self-aggregation is 58.98%, demonstrating that *Lactobacillus plantarum* SWUN3927 of this invention has good intestinal adhesion and possesses the potential of a probiotic. This invention verifies that *Lactobacillus plantarum* SWUN3927 can effectively regulate animal immunity, improve the body's antioxidant capacity, increase lysozyme content and alkaline phosphatase activity, improve daily weight gain, and ultimately improve animal growth performance.

This invention provides a probiotic symbiotic biofilm and its application in the preparation of products for the prevention or adjunctive treatment of alcoholic liver disease, relating to the field of biomaterials technology. The probiotic symbiotic biofilm is composed of *Lactobacillus rhamnosus* (…). Lactobacillus rhamnosus LRa05 and Bifidobacterium lactis ( Bifidobacterium lactis The above two microorganisms were prepared using BLa80. Under co-culture conditions, they can form a dense symbiotic biofilm. The two strains are evenly distributed and intertwined within the biofilm, forming a thicker and more stable three-dimensional structure than a single strain. This symbiotic biofilm structure endows the probiotics with stronger environmental tolerance and intestinal adhesion ability, laying the foundation for their colonization and sustained function in the intestine. It can not only significantly improve alcohol-induced intestinal barrier damage, but also effectively intervene in alcoholic liver disease, providing a new application scenario for the two strains.

This invention discloses a probiotic sustained-release microsphere for the gastrointestinal tract and its preparation method, belonging to the field of microbial preparation technology. The microsphere has a multi-layered structure, consisting of, from the inside out: a core layer containing probiotics, juglone-7-O-β-D-glucuronide, and γ-polyglutamic acid; an inner wall layer composed of calcium alginate-low methoxy pectin gel; and an outer adhesion layer formed by alternating assembly of cysteine-modified chitosan and fucoidan. In preparation, a core material mixture and a composite wall material solution are first mixed to form an embedding solution, which is then electrostatically sprayed to solidify into primary gel microspheres. These microspheres are then sequentially immersed and coated with cysteine-modified chitosan solution and fucoidan solution, and dried to obtain the final product. The microspheres of this invention effectively protect probiotics from damage caused by gastric acid and a simulated digestive environment, achieving sustained-release, significantly improving the freeze-dried survival rate of probiotics, and contributing to enhanced intestinal adhesion potential and local retention capacity. They are suitable for products regulating gastrointestinal function.