Connective tissue disease-associated early interstitial lung disease diagnostic kit

A technology of pulmonary interstitial lesions and diagnostic kits, applied in disease diagnosis, measuring devices, instruments, etc., can solve problems such as difficult to repeat, difficult to accept by patients, difficult to accept by patients, etc., and achieve the effect of easy operation

Inactive Publication Date: 2014-04-02
陈娟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

HRCT detection method is too expensive, and it is not easy to repeat, it cannot detect early ILD, and it is not easy to conduct a general survey of connective tissue diseases; lung biopsy is an invasive surgical examination, which is not easy for patients to accep

Method used

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  • Connective tissue disease-associated early interstitial lung disease diagnostic kit
  • Connective tissue disease-associated early interstitial lung disease diagnostic kit
  • Connective tissue disease-associated early interstitial lung disease diagnostic kit

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A real case study was conducted on 135 patients with rheumatoid arthritis (RA), and it was confirmed that matrix metalloproteinase 7 (MMP7), interferon-γ (IFN-γ) and chemotaxis in peripheral serum of patients with connective tissue disease pulmonary interstitial lesions Increased levels of factor IP-10. Detection of matrix metalloproteinase 7 (MMP7), interferon-γ (IFN-γ) and chemokine IP-10 levels in peripheral serum of patients can diagnose early pulmonary interstitial lesions in connective tissue diseases. The specific verification process is as follows:

[0033] 135 patients with rheumatoid arthritis (RA) were recruited from the Department of Rheumatology and Immunology of the First Affiliated Hospital of Xiamen University. All were in line with the 1982 American College of Rheumatology diagnostic criteria for RA. All enrolled patients underwent high-resolution lung CT (HRCT) and pulmonary function (PFTs) examinations, and statistics of clinical joint activity indi...

Embodiment 2

[0046] A diagnostic kit for early pulmonary interstitial lesions of connective tissue diseases made according to the experimental results of Example 1 above, including a first ELISA detection component for detecting peripheral serum MMP7 in patients with connective tissue diseases, and a second ELISA for detecting IFN-γ Detection components and a third ELISA detection component to detect IP-10.

[0047] The preparation method of the above-mentioned 96-well detection plate is as follows:

[0048] Coat 200ng / mL antibody on a 96-well plate at 100uL per well, the antibody diluent is PBS, incubate the 96-well plate at 37°C for 2 hours; incubate for two hours, wash the plate once, add 200uL of blocking solution to each well, Block at 37°C for 2 hours, wash the plate once after sealing, and place the plate in a drying room for later use; the composition of the blocking solution is: 1XPBS + 0.5% casein + 2% gelatin + 0.1% preservative (proclin-300).

[0049] The detection process of ...

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Abstract

The invention discloses a connective tissue disease-associated early interstitial lung disease diagnostic kit, comprising a detection module for detecting human peripheral blood MMP7, IFN-gamma and IP-10. The commercial kit provided by the invention is sensitive, safe, reliable and easy to operate; the kit is capable of quantificationally measuring the level of specific protein cytokines and chemotactic factors in human serum, and is helpful for early diagnosis of the connective tissue disease-associated interstitial lung disease.

Description

technical field [0001] The invention specifically relates to a diagnostic kit for early pulmonary interstitial lesions of connective tissue diseases. Background technique [0002] Interstitial lung disease (ILD) is an inflammatory disease of the pulmonary interstitium caused by a variety of reasons, which progresses slowly and gradually loses alveolar-capillary functional units, and eventually develops into diffuse pulmonary fibrosis and honeycombing. cause respiratory failure and death. In the early clinical stage, it manifests as shortness of breath after activity. As the disease progresses, it manifests as a series of symptoms such as dyspnea, cardiac insufficiency, and repeated lung infections. It is a group of diseases with high incidence and poor prognosis, and its median survival time is only 2 -3 years. There are many diseases that lead to pulmonary interstitial disease, such as idiopathic interstitial pneumonia (IIP), in which connective tissue disease (CTD) is an...

Claims

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Application Information

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IPC IPC(8): G01N33/535G01N33/531
CPCG01N33/6887G01N2800/10G01N2800/12
Inventor 陈娟史永红王小平葛胜祥刘永亮
Owner 陈娟
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