Y-STR locus fluorescent label multiplex amplification system and application thereof

Abstract

The invention relates to a Y-STR locus fluorescent label multiplex amplification system and an application thereof, and belongs to the field of polymorphic marker in detected human genome. The multiplex amplification system can simultaneously amplify loca of DYS19, DYS389-1, DYS389-2, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, DYA7.2 and Amelogenin. In the meanwhile, through the design of specific primers and a unique locus combination fluorescent label mode, only three marks can be adopted to simultaneously amplify the above loca, and amplified products are all controlled between 100bp to 400bp with very high sensitivity. A kit designed according to the amplification system is simple and rapid to operate, has high sensitivity, is high efficient, saves templates, has high individual identification capability, and reaches the level of foreign commercial kits of the same type.

Description

technical field [0001] The invention relates to the detection of polymorphic genetic markers in the human genome, in particular to a Y chromosome STR locus fluorescent marker compound amplification system and its application. Background technique [0002] Since DNA polymorphism analysis technology was applied to individual identification and paternity test of forensic biological evidence in 1985, it has greatly promoted the development of forensic evidence identification technology and led to an epoch-making breakthrough in criminal technology. Over the past ten years, new DNA polymorphism analysis methods have emerged continuously, many new polymorphic ideal genetic markers have been developed, and a series of modern DNA testing techniques have been born. In particular, the STR locus testing technology that began to appear in 1992 quickly became the mainstream technology in forensic DNA identification due to its good polymorphism, strong recognition ability, high sensitivit...

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Problems solved by technology

[0004] Problems in the prior art: 1. At present, domestic research reports on Y-STR mainly focus on the development of new loci, population genetics investigations and some method research, but mainly use the detection methods of single locus test and silver staining. There is no ready-made kit for fluorescently labeled multi-locus multiplex test

2. Relevant research in foreign countries started earlier, and the research is relatively in-depth. At present, the Y-STR fluorescent labeling multiplex amplification kit has been launched, but the Y-STR loci used in the foreign kits are selected according to the genetic data of European and American populations. In practical applications, some gene loci are not suitable for the Chinese population, and the price is expensive

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