Y-STR locus fluorescent label multiplex amplification system and application thereof
A compound amplification system and fluorescent labeling technology, which is applied in the field of fluorescence labeling compound amplification system of Y chromosome STR loci, can solve the problems of high price and unsuitable gene locus for Chinese population, and achieve accurate typing results and high sensitivity Effect
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[0058] The kit includes: primer mixtures for 10 Y-STR loci and the sex identification locus Amelogenin, PCR reaction buffer, Taq Gold DNA polymerase and allelic standards for each locus.
[0059] 1. Selection of Y-STR loci and design and synthesis of primers
[0060] According to the report of the genetic data of the Chinese population, a total of 10 Y-STR loci and sex identification loci Amelogenin were selected, including DYS19, DYS389-1, DYS389-2, DYS390, DYS391, DYS392, DYS393, DYS438, DYS439, and DYSA7.2. Synthesize PCR amplification primers, and label the 5' end of a primer in each pair of primers with fluorescence. The primer sequences and label fluorescence of each gene locus are shown in Table 1:
[0061] Table 1: Primers and fluorescent dye labels for each locus
[0062]
[0063] 2. Composition and reaction parameters of each component in the PCR amplification reaction system of the kit
[0064] The PCR reaction system is 10ul, including primer pair mixture 1.0u...
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