Method of detecting beta-adrenergic receptor agonist in animal tissue

A receptor agonist and epinephrine technology, applied in the field of adrenergic receptor agonist drugs, can solve the problems of complex steps, inability to obtain information on the spatial distribution of drug molecules, shortening time, avoiding information loss, and reducing costs Effect

Active Publication Date: 2019-08-23
广东粤器科技有限公司
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

The analysis of β-adrenergic receptor agonist drugs by traditional LC-MS method requires pretreatment of tissue samples, which is complicated and the spatial distribution information of drug molecules in tissues cannot be obtained due to tissue processing.

Method used

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  • Method of detecting beta-adrenergic receptor agonist in animal tissue
  • Method of detecting beta-adrenergic receptor agonist in animal tissue
  • Method of detecting beta-adrenergic receptor agonist in animal tissue

Examples

Experimental program
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Embodiment 1

[0026] Example 1: Detection of Bambuterol in Rat Tissue:

[0027] Apparatus: High-resolution mass spectrometer SYNAPT G2-Si (WATERS Corporation) equipped with a desorption electrospray ionization ion source (WATERS Corporation).

[0028] Sample processing: The rats were weighed and anaesthetized with chloral hydrate (150mg / ml, 0.2ml / 100g); the rats were divided into three groups: 15min, 30min and 45min groups. After 15min, 30min and 45min administration of bambuterol, the rats were perfused the whole body respectively, and the tissues were taken, and then the tissue samples were frozen sectioned and placed on glass slides.

[0029] Standard preparation: take another blank rat tissue, weigh the tissue, add 1 volume of normal saline to grind; make bambuterol with normal saline to make 20μg / ml, 2μg / ml, 200ng / ml and 20ng 50 μl of tissue homogenate were placed in 5 ep tubes, and 50 μl of different concentrations of medicinal liquid and normal saline were added respectively, and th...

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Abstract

The invention belongs to the technical field of animal tissue clenbuterol detection, and discloses a method of detecting beta-adrenergic receptor agonist in an animal tissue. The method is simple to operate, the beta-adrenergic receptor agonist in the animal tissue can be quickly found out, and spatial distribution detection on the beta-adrenergic receptor agonist in the tissue sample is realized.The method comprises steps of animal tissue sample taking, to-be-detected sample preparation (comprising tissue frozen section and standard preparation), desorption electrospray ionization mass spectrometry and imaging result analysis.

Description

technical field [0001] The invention belongs to the technical field of animal tissue clenbuterol detection, in particular to a method for detecting beta-adrenergic receptor agonist drugs in animal tissue by using desorption electrospray ionization mass spectrometry technology. Background technique [0002] Salbutamol, terbutaline, bambuterol, clenbuterol, etc. belong to the class of beta-adrenergic receptor agonists, which are used in veterinary medicine and medicine to treat bronchospasm and shock. Studies have shown that these drugs have the effect of promoting protein accumulation and muscle growth in animals. However, excessive use makes the drug accumulate in animal tissues of livestock, and the consumption of such animal tissues is harmful to human health. The state strictly controls the residues of β-adrenergic receptor agonist drugs in animal tissues. Therefore, the detection of β-adrenergic receptor agonists in animal tissues is particularly important. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62G01N33/15
CPCG01N27/62G01N33/15
Inventor 武杰张睿谭文
Owner 广东粤器科技有限公司
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