Predicting method for impact energy needed for quantifying content of traditional Chinese medicine of triterpenoid saponin on basis of electrospray protonation cracking

A technology of triterpenoid saponins and prediction methods, which can be applied to measurement devices, instruments, scientific instruments, etc., and can solve problems such as time-consuming and labor-intensive

Active Publication Date: 2018-11-30
夏永刚
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Problems solved by technology

In the early stage, the inventor's research group optimized CE through traditional syringe pump method, single factor experiment method and response surface method. However, when multiple triterpenoid saponins are analyzed at the same time, it is time-consuming and laborious to use these...

Method used

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  • Predicting method for impact energy needed for quantifying content of traditional Chinese medicine of triterpenoid saponin on basis of electrospray protonation cracking
  • Predicting method for impact energy needed for quantifying content of traditional Chinese medicine of triterpenoid saponin on basis of electrospray protonation cracking
  • Predicting method for impact energy needed for quantifying content of traditional Chinese medicine of triterpenoid saponin on basis of electrospray protonation cracking

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experiment example 1

[0035] Experimental example 1 Quantitative ideal collision energy prediction experiment of 85 triterpene saponins based on protonation fragmentation ion pairs

[0036] 1 experimental equipment

[0037] Qtrap-4000 triple quadrupole linear ion trap mass spectrometer (AB SCIEX company, the United States); H-class ultra-high performance liquid chromatography (online degasser, automatic sampler, quaternary pump, American Waters company); ACQUITY UPLC HSS T3 chromatographic column (2.1×150mm, 1.8μm), connected to a Waters HSS T3 guard column (2.1×5mm, 1.8μm). Milli-Q water purifier (Millipore China Co., Ltd.), ML104 / 02 electronic analytical balance (Mettler Toledo Instruments Shanghai Co., Ltd.), Vortex 3000 vortex oscillator (Wiggens, Germany); Mikro 200R centrifugal Machine (Hettich, Germany), KQ-500DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.).

[0038] 2 Preparation of standard products

[0039] Accurately weigh triterpenoid saponin reference substance 1...

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Abstract

The invention discloses a predicting method for impact energy needed for quantifying the content of a traditional Chinese medicine of triterpenoid saponin on the basis of electrospray protonation cracking. The predicting method comprises the steps that (1) medicinal plants adopting the triterpenoid saponin as a main active constituent are selected as representative plants; (2) triterpenoid saponinmodel compounds with representativeness are selected from the representative plants; and (3) the content of the triterpenoid saponin in the medicinal plants is quantified on the basis of electrosprayprotonation cracking, and the needed impact energy is predicted through the following formulas of CE=0.0337*Q1+0.5686 and CE=0.0337*Mw+0.9361, wherein Q1 is equal to [M+H/NH4]+, the linear range of Q1 is 600-1600 Da, R2 is equal to 0.91, the linear range of Mw is 600-1600 Da, and R2 is equal to 0.9096. The CE value obtained through the predicting method and the CE value obtained through a singlefactor investigation method have no significant difference, and it is explained that the predicting method can be applied to detecting of a triple quadrupole mass spectrum.

Description

technical field [0001] The present invention relates to a method for predicting the collision energy required for analyzing and quantifying the content of triterpene saponins in traditional Chinese medicine, in particular to a method for rapidly predicting the ideal collision energy required for quantifying triterpene saponins in traditional Chinese medicine based on electrospray protonation cracking-multiple reaction monitoring mass spectrometry, The invention belongs to the field of quantitative detection of triterpenoid saponin content in traditional Chinese medicine. Background technique [0002] Triterpene saponins form stable [M+H] in mobile phases containing formic acid or ammonium formate + and / or [M+NH 4 ] + Molecular ion peaks, using liquid chromatography-tandem quadrupole mass spectrometry, using electrospray positive ion tandem mass spectrometry detection mode (ESI + -MRM), which identified predictable MRM-targeted transitions whose Q 1 and Q 3 The calculati...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 夏永刚梁军郭信东宋艳
Owner 夏永刚
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