Photo ionization mass spectrum imaging device combing with desorption electrospray ionization

A desorption electrospray and electrospray ionization technology, applied in the field of mass spectrometry imaging, can solve the problems of low ionization efficiency, discrimination, and high sensitivity of neutral molecules, and achieve the effects of increased sensitivity, strong matrix effect, and improved detection sensitivity

Pending Publication Date: 2018-11-09
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are some problems in analytical electrospray ionization: (1) It has polarity discrimination, and the ionization efficiency is low for weakly polar neutral molecules; (2) It is highly sensitive to charged compounds, and charged compounds in biological tissues will inhibit The mass spectrometry signal of neutral compounds, that is, ion suppression; (3) Biological tissues contain a large amount of sodium and potassium salts, and the ionization efficiency of electrospray ionization is low in salt-containing samples, so there will be many unionized neutral compound molecules along with the ions together resolve from the sample surface into the mass spectrometer

Method used

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  • Photo ionization mass spectrum imaging device combing with desorption electrospray ionization
  • Photo ionization mass spectrum imaging device combing with desorption electrospray ionization
  • Photo ionization mass spectrum imaging device combing with desorption electrospray ionization

Examples

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Embodiment 1

[0032] see figure 1 , a photoionization mass spectrometer imaging device combined with desorption electrospray ionization includes a mass spectrometer, an analytical electrospray ionization mechanism and a photoionization mechanism.

[0033] The electrospray ionization mechanism includes an atomizer 2, the inlet of the atomizer 2 is connected to the syringe 3 through the feed capillary 1, the outlet of the atomizer 2 is connected to the electrospray needle 4, and the middle part of the atomizer 2 is connected to the atomizer The trachea 10 ; the outlet end of the electrospray needle 4 corresponds to the workbench of the two-dimensional stepping motor 6 .

[0034] see image 3 , The photoionization mechanism includes a transfer mechanism 8 and a light source mechanism.

[0035]The transfer mechanism 8 includes a tubular photoionization chamber 82 and a sampling capillary 81. One end of the photoionization chamber 82 is a sample outlet, which is sealed and connected to the tr...

Embodiment 2

[0042] The structure of the device used in this embodiment 2 is the same as that of embodiment 1, and the different parameters are as follows: the distance between the inner diameter of the photoionization chamber 82 and the outer diameter of the draft tube 83 is 1.0 mm, and the inner diameter of the photoionization chamber 82 is 4mm ; The inner diameter of the draft tube 83 is 1.2 mm; the inner diameter of the sampling capillary 81 is 1.0 mm. The angle between the sampling capillary 81 and the slide glass 5 is 12°; the angle between the electrospray needle 4 and the slide glass 5 is 53°, and the horizontal distance between the outlet end of the electrospray needle 4 and the sampling capillary 81 is 20 mm.

[0043] see figure 2 and Figure 4 , the light source mechanism is a vacuum ultraviolet light source mechanism 9, including a vacuum ultraviolet lamp 91, the vacuum ultraviolet lamp is sealed and connected to the photoionization chamber 82 through an upper cover plate 92 ...

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Abstract

The invention relates to a photo ionization mass spectrum imaging device combing with desorption electrospray ionization, comprising a mass spectrometer and a desorption electrospray ionization mechanism; the improvement lies in that a photo ionization mechanism is additionally arranged, and comprises a changeover mechanism and a light source mechanism; the light source mechanism is a synchronousradiation light and laser photo ionization mechanism or a vacuum ultraviolet light source mechanism. In detection and analysis, a solvent flows out from the desorption electrospray ionization mechanism to be used for desorbing a compound in a biological tissue on a glass slide, and the desorbed compound enters the photo ionization mechanism, is ionized by synchronous radiation light and laser or vacuum ultraviolet light, and enters mass spectrum to be analyzed and imaged. According to the invention, mass spectrum information is combined with movement of a two-dimensional sample moving platformto realize synchronous imaging of polar and non-polar compounds in the biological tissue, and in combination with the advantage of photo ionization, the device has wide prospects in the aspects of physiological disease, tumor research and the like in the future.

Description

technical field [0001] The invention relates to the technical field of mass spectrometry imaging, in particular to a photoionization mass spectrometry imaging device combined with desorption electrospray ionization. Background technique [0002] Mass spectrometry imaging technology is an imaging method in which the sample platform moves according to certain rules under the control of software programs, directly scans biological samples for imaging through mass spectrometry, and analyzes the spatial distribution of biomolecules according to the measured mass-to-charge ratio (m / z). Compared with traditional optical biological imaging technology, mass spectrometry imaging technology belongs to molecular information imaging, and is a new analysis technology for studying molecular imaging in biological tissues and living animals. Compared with traditional fluorescent molecular imaging and immunolabeling molecular imaging techniques, mass spectrometry imaging can realize molecular...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/64
CPCG01N27/64
Inventor 潘洋刘成园杨玖重戚可可许鸣皋臧建业熊英
Owner UNIV OF SCI & TECH OF CHINA
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