Method for simultaneously detecting six types of 24 antibiotics in water by solid phase extraction-liquid chromatography-tandem mass spectrometry
A technology of liquid chromatography and tandem mass spectrometry, which is applied in the field of detection of trace organic pollutant residues, can solve the problems of interfering with multiple detection of antibiotic quantity and range, large consumption of organic reagents, and long time consumption, so as to avoid unnecessary damage and reduce processing time. Short, reduce the effect of matrix effect
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Embodiment 1
[0038] The present invention is a method for simultaneously detecting six types of 24 antibiotics in a water body by solid-phase extraction-liquid chromatography tandem mass spectrometry, which is specifically implemented according to the following steps:
[0039] Step 1. Pretreatment of water samples
[0040] Collect drinking water, tap water, surface water, and influent and effluent water from sewage treatment plants, respectively. The water samples are filtered through a 0.45 μm glass fiber membrane to remove suspended solids, and then accurately measure 1 L with a graduated cylinder. Use dilute sulfuric acid with a volume concentration of 10% to adjust the pH of the water sample to 3.0-4.0 (if the surface water is collected to inhibit the degradation of antibiotics by microorganisms, a certain amount of dilute hydrochloric acid will be added, and the pH adjustment step can be omitted at this time). Before solid phase extraction, 10 μL and 5 mg / L of 13 C 1 D 3 - Erythrom...
Embodiment 2-3
[0087] Example 2-3 Determination of the concentration of 24 antibiotics in two different actual water bodies
[0088] Examples 2 and 3 respectively take surface water and sewage from livestock and poultry farms as examples. First, the method of step 1 is used for pretreatment, and then the method of step 2 is used for solid-phase extraction, enrichment and purification pretreatment, and then step 3 is used. The established detection method is detected and analyzed to examine the applicability of the established detection method in step 3 of the present invention.
[0089] A kind of solid-phase extraction-liquid chromatography tandem mass spectrometry method of the present invention described in embodiment 2 and embodiment 3 simultaneously detects six types of 24 kinds of antibiotics in water body, and is specifically implemented according to the following steps:
[0090] Step 1. Pretreatment of water samples
[0091] Collect surface water and livestock and poultry farm sewage...
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