Preparation method of vegetable protein polypeptide liquid

Abstract

The invention discloses a preparation method of vegetable protein polypeptide liquid. The preparation method comprises the following steps: (1) activating; (2) carrying out pre-enzymolysis; (3) carrying out secondary enzymolysis; and (4) filtering. According to the preparation method disclosed by the invention, soybean protein powder and peanut protein powder are used as raw materials, and are activated to improve the reaction activity of the protein powder; the pre-enzymolysis and the secondary enzymolysis are adopted, so that the content of soluble nitrogen in slurry is remarkably improved and the content of the nitrogen can reach 1.5%; the enzymolysis efficiency is high, the enzymolysis degree of protein is high and the recycling rate of the protein is up to 87.6%; the peptide yield is high and the balance of amino acids is good; abundant carbon and nitrogen sources, and growth factors can be provided for the growth of microorganisms; purified water is added for two times and time and energy sources are saved, so that the preparation method has remarkable economic and social benefits; vegetable protein polypeptide liquid prepared by the invention can be directly used for preparing a streptococcus fermentation culture medium, and does not need to be concentrated, and spray-dried, so that a lot of energy sources are saved and the production cost is reduced, and furthermore, the preparation method is suitable for industrial large-scale production.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a preparation method of vegetable protein polypeptide liquid. Background technique [0002] Peptone is the main raw material of microbial culture medium. It is widely used in the fields of antibiotics, pharmaceutical industry, fermentation industry, biochemical products and microbiological research. Peptone is divided into plant peptone and animal peptone according to the source of protein. The sources of animal peptone raw materials are complex, resulting in large differences in the proportions of various amino acids in peptone products, which affect the yield and quality of fermentation end products. Fermentation end products of raw materials are favored by many high-end customers. [0003] At present, the price of foreign brand plant peptone supplied in the market is high, while the production level of domestic plant peptone enterprises is uneven, and most...

AI Technical Summary

Problems solved by technology

The sources of animal peptone raw materials are complex, resulting in large differences in the proportions of various amino acids in peptone products, which affect the yield and quality of fermentation end products. Fermentation terminal products of raw materials are favored by many high-end customers

[0003] At present, the price of foreign brand plant peptone supplied in the market is high, while the production level of domestic plant peptone enterprises is uneven, and most of them use a single plant protein raw material to be prepared by a single enzymatic hydrolysis method, the yield of protein polypeptide is low, and the proportion of amino acids is balanced The quality of plant peptone is poor, and there is a big gap with the quality of foreign plant peptone products