Method for preparing whey protein peptide by composite protease hydrolysis

A compound protease and whey protein peptide technology, which is applied in the field of preparation of biologically active short peptides, can solve the problems of immature detection methods and technologies, difficulty in separation and purification, and difficulty in industrial production, and achieve excellent molecular weight distribution range, peptide High content, good product quality effect
CN101260421AInactive Publication Date: 2008-09-10ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Publication Date
2008-09-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for preparing lactalbumin peptides, comprising the following steps: (1) adding compound protease into lactalbumin solution to carry out enzymatic hydrolysis; (2) inactivating the compound protease; (3) depositing the enzyme solution and selecting supernatant fluid to carry out centrifugation; (4) carrying out decoloring processing on the centrifugalized supernatant fluid and then carrying out desalting processing; (5) carrying out nanofiltration on the desalted enzyme solution; (6)concentrating; (7) embedding the concentrated product into wall material; (8) spray drying, then the product is obtained. By applying compound protease in the invention, the lactalbumin is zymohydrolyzed with effectively improving enzyme hydrolysis degree, prominently improving usage factor of the lactalbumin, high product yield, good distribution range of molecular weight of the lactalbumin peptides, high peptide content and good product quality. By detecting, the content (counted by dry basis) of the lactalbumin peptides prepared by the method in the invention is up to 75.15 percent, the molecular weight distribution range of the peptide smaller than 2258 dalton can reach 98.83 percent.
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Description

Technical field

[0001] The present invention relates to a method for preparing short biologically active peptides, in particular to a method for preparing whey protein peptides by compound enzymatic hydrolysis of whey protein, belonging to the field of biochemistry. Background technique

[0002] Milk contains a large number of biologically active peptides with different activities. These peptides are either naturally present in milk or derived from the products of milk protein hydrolysis. Due to its small molecular weight, it is absorbed by the human body in the form of a complete peptide in the small intestine, will not cause immune rejection in the human body, and has a variety of special physiologically active functions.

[0003] Whey protein is hydrolyzed by protease to obtain small molecule whey protein peptides. Whey protein peptides are mainly a mixture of peptides composed of 2-7 amino acids. It is a multifunctional nutritional factor with immune regulation, anti-thrombot...

Claims

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