Dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and preparation method

A technology of gold nanoclusters and fluorescent probes, applied in chemical instruments and methods, luminescent materials, fluorescence/phosphorescence, etc., can solve the problems of complex synthesis and modification process, poor water solubility and photostability, and low quantum yield

Inactive Publication Date: 2015-11-18
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, fluorescent probes based on synthetic organic dyes have been designed and used for Hg 2+ However, such probes have problems such as complex synthesis and modification process, low quantum yield, poor water solubility and photostability, which limit their practical application.
Previously reported merc...

Method used

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  • Dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and preparation method
  • Dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and preparation method
  • Dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of aminated fluorescent silica nanoparticles coated with carbon dots

[0027] The siloxane functionalized carbon dots are prepared by adopting the method of the published Chinese patent (publication number: CN103421495A); other preparation methods are not limited.

[0028] In this experiment, silica nanoparticles coated with carbon dots were prepared by inverse microemulsion method. The specific experimental steps are as follows: add 7.7mL cyclohexane, 1.77mL Triton X-100 and 1.77mL n-hexanol to a 25mL round bottom flask respectively, then add 380μL water, and stir the system vigorously for about 10min to form a stable and uniform After the inverse microemulsion system is formed, add 50 μL tetraethyl orthosilicate to the flask, stir evenly, add 25 μL new carbon quantum dots and 200 μL ammonia water (25wt%) to it, and place the reaction system React at room temperature; after reacting for 18 hours, add isopropanol three times the volume of the reaction s...

Embodiment 2

[0036] (1) Preparation of aminated fluorescent silica nanoparticles coated with carbon dots

[0037] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0038] In this experiment, silica nanoparticles coated with carbon dots were prepared by inverse microemulsion method. The specific experimental steps are as follows: add 7.7mL cyclohexane, 1.77mL Triton X-100 and 1.77mL n-hexanol to a 25mL round bottom flask respectively, then add 380μL water, and stir the system vigorously for about 10min to form a stable and uniform After the inverse microemulsion system is formed, add 50 μL tetraethyl orthosilicate to the flask, stir evenly, add 25 μL new carbon quantum dots and 200 μL ammonia water (25wt%) to it, and place the reaction system React at room temperature; after reacting for 20 hours, add isopropanol three times the volume of the reaction solution to the reaction system to break the em...

Embodiment 3

[0046] (1) Preparation of aminated fluorescent silica nanoparticles coated with carbon dots

[0047] Siloxane functionalized carbon dots were prepared by adopting the method of the published Chinese patent (publication number: CN103421495A).

[0048] In this experiment, silica nanoparticles coated with carbon dots were prepared by inverse microemulsion method. The specific experimental steps are as follows: add 7.7mL cyclohexane, 1.77mL Triton X-100 and 1.77mL n-hexanol to a 25mL round bottom flask respectively, then add 380μL water, and stir the system vigorously for about 10min to form a stable and uniform After the inverse microemulsion system is formed, add 50 μL tetraethyl orthosilicate to the flask, stir evenly, add 25 μL new carbon quantum dots and 200 μL ammonia water (25wt%) to it, and place the reaction system React at room temperature; after reacting for 24 hours, add isopropanol three times the volume of the reaction solution to the reaction system to break the em...

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Abstract

The invention relates to a dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and a preparation method. Dual-emission composite silicon dioxide nanoparticles are composite silicon dioxide nanoparticles formed by utilizing carbon dot covered silicon dioxide particles as cores and covalently coupling the surfaces of the carbon dot covered silicon dioxide particles with the Au nanoclusters after surface amination. The carbon dots located in the silicon dioxide nanoparticle cores are taken as reference fluorescence signals, the Au nanoclusters on the outer layers are taken as response fluorescence signals, and the signals are used for Hg<2+> selective recognition. The Au nanoclusters as the response fluorescence signals are connected to the surface of a silicon layer through covalent bond connection, and one stable nano-fluorescent probe is formed. When the dual-fluorescence composite nanoparticles are taken as the rate type fluorescent probes, the intensity of the carbon dot fluorescence signals in the cores basically keep unchanged, and the Au nanoclusters on the outer layers can be bonded with Hg<2+> selectively so as to result in fluorescence quenching of the Au nanoclusters on the outer layers.

Description

technical field [0001] The present invention relates to a fluorescent probe and a preparation method thereof, in particular to a fluorescent probe for the visual analysis and detection of heavy metal ion mercury ions and a preparation method thereof, specifically a carbon dot-gold nanocluster dual A fluorescent probe for detecting mercury ions by emitting fluorescent signals and a preparation method thereof. Background technique [0002] The pollution of heavy metal ions has always been a very serious environmental and ecological problem. Especially mercury ions (Hg 2+ ) The pollutants represented by ) will not decompose quickly once they enter the environment, but remain in the soil, water, and air for a long time, resulting in enduring pollution. Mercury ions entering the human body also have great harm to the health of the human body. For example, mercury ions have serious damage to the human nervous system, hematopoietic system, respiratory system and kidneys. Therefo...

Claims

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Application Information

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IPC IPC(8): G01N21/64C09K11/06
Inventor 苏荣欣高召黄仁亮齐崴王梦凡
Owner TIANJIN UNIV
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