Dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and preparation method

Abstract

The invention relates to a dual emission rate type fluorescent probe for visually detecting carbon dots-Au nanoclusters of mercury ions and a preparation method. Dual-emission composite silicon dioxide nanoparticles are composite silicon dioxide nanoparticles formed by utilizing carbon dot covered silicon dioxide particles as cores and covalently coupling the surfaces of the carbon dot covered silicon dioxide particles with the Au nanoclusters after surface amination. The carbon dots located in the silicon dioxide nanoparticle cores are taken as reference fluorescence signals, the Au nanoclusters on the outer layers are taken as response fluorescence signals, and the signals are used for Hg<2+> selective recognition. The Au nanoclusters as the response fluorescence signals are connected to the surface of a silicon layer through covalent bond connection, and one stable nano-fluorescent probe is formed. When the dual-fluorescence composite nanoparticles are taken as the rate type fluorescent probes, the intensity of the carbon dot fluorescence signals in the cores basically keep unchanged, and the Au nanoclusters on the outer layers can be bonded with Hg<2+> selectively so as to result in fluorescence quenching of the Au nanoclusters on the outer layers.

Description

technical field [0001] The present invention relates to a fluorescent probe and a preparation method thereof, in particular to a fluorescent probe for the visual analysis and detection of heavy metal ion mercury ions and a preparation method thereof, specifically a carbon dot-gold nanocluster dual A fluorescent probe for detecting mercury ions by emitting fluorescent signals and a preparation method thereof. Background technique [0002] The pollution of heavy metal ions has always been a very serious environmental and ecological problem. Especially mercury ions (Hg 2+ ) The pollutants represented by ) will not decompose quickly once they enter the environment, but remain in the soil, water, and air for a long time, resulting in enduring pollution. Mercury ions entering the human body also have great harm to the health of the human body. For example, mercury ions have serious damage to the human nervous system, hematopoietic system, respiratory system and kidneys. Therefo...

AI Technical Summary

Problems solved by technology

In recent years, fluorescent probes based on synthetic organic dyes have been designed and used for Hg 2+ However, such probes have problems such as complex synthesis and modification process, low quantum yield, poor water solubility and photostability, which limit their practical application.

Previously reported mercury ion fluorescent probes generally adopt a single fluorescence quenching mode, and the measurement results are easily affected by factors such as the concentration of the detected substance, external environment, and instrument conditions, which will cause data distortion and reduce the accuracy of the measurement results.

Images