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Surface enhanced spectrometry-active composite nanoparticles

A nanoparticle, metal nanoparticle technology, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, and can solve problems such as limited application and extremely weak Raman

Inactive Publication Date: 2007-08-15
BECTON DICKINSON & CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the Raman spectrum generally covers 200-3500cm -1 The vibration energy and because each vibration generally has a very narrow bandwidth, that is, -1 , so it can be expected to use a single light source to simultaneously measure a dozen (or more) reporter molecules. However, normal Raman is extremely weak, which limits its application in bioanalytical chemistry

Method used

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Examples

Experimental program
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Embodiment 1

[0278] A typical synthesis of ~45 nm diameter Au colloids using a combination of sodium borohydride, sodium citrate and hydroxyamide hydrochloride is described. Wash all glassware with aqua regia and rinse thoroughly with 18 MΩ water. Reactions were performed in a cold room. Prepare 0.01% (w / w) HAuCl 4 ·3H 2 Aqueous solution of O, 8% (w / w) solution of sodium citrate dihydrate in 0.01N NaOH, 10 -4 % sodium borohydride in 0.01 N NaOH and 400 mM hydroxyamide hydrochloride in water. A mixture of citric acid and borohydride solution was then prepared by combining 1 mL of sodium citrate solution with 100 μl of sodium borohydride solution and 500 μl of 0.01N NaOH. After preparing this mixture, inject 200 μl of the hydroxyamide solution into 100 mL of HAuCl in a 250 mL Erlenmeyer flask 4 solution and stirred briefly. After 20 min, a borohydride / citrate mixture such as rapidly stirred HAuCl 4 and hydroxyamide solution. Best results were obtained when injecting in the mid-range ...

Embodiment 2

[0280] Preparation of gold nanoparticles. Prepared in H 2 1% or 2% concentration (w / v) of HauCl in O 4 ·3H 2 O stock solution. These solutions were filtered through a 0.2 μm membrane, after which they were placed in a cold room. Additionally, the bottles containing these stock solutions are generally covered with aluminum foil to reduce exposure to light. Typically the following solutions are prepared:

[0281] 1. In H 2 1.0 L 0.02% HauCl in O 4 .

[0282] 2.5 mL of 32% (w / v) sodium citrate dehydrate in 0.01N NaOH.

[0283] 3.10mL 1.6M in H 2 Hydroxyamide hydrochloride in O.

[0284] 4. 10 mL of 4% (w / v) NaBH in 1.0 N NaOH prepared by diluting the stock solution 4 .

[0285] All reactions were performed in a cold room after the solutions were brought to room temperature. HauCl 4 The solution was placed in a 2L round bottom flask and a glass stir bar with a Teflon paddle was inserted into the flask to provide stirring. Add 1.0 mL of the hydroxyamide solution to t...

Embodiment 3

[0287] SERS spectra of SACN labeled with 4-mercaptopyridine (4-MP) and trans-4,4′-bis(pyridyl)ethylene (BPE).

[0288] Materials: Water used for all preparations was 18.2 MΩ and was distilled by a Barnstead Nano Purity System. 3-Aminopropyltrimethoxysilane (APTMS), HAuCl 4 ·3H 2 O, trisodium citrate dihydrate, sodium hydroxide, sodium borohydride, hydroxyamide hydrochloride, trans-1,2-bis(4-pyridyl)ethylene (BPE), 4-mercaptopyridine, silicic acid Sodium, tetraethylorthosilicate (TEOS), ethanol and 2.0 M ammonia in ethanol were obtained from Sigma-Aldrich. BPE was recrystallized before use.

[0289] Colloid preparation: by HAuCl 4 ·3H 2 O to prepare 35nm colloidal Au. As sodium borohydride in 0.001N NaOH 10 -2 % solution, an aqueous solution of 4% sodium citrate and 400 mM hydroxyamide hydrochloride was prepared immediately prior to synthesis. Aliquot 300 μl of this borohydride solution with 500 μl citrate and 350 μl hydroxyamide and immediately inject 200 mL 0.01% HAuC...

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Abstract

Submicron-sized particles or labels that can be covalently or non-covalently affixed to entities of interest for the purpose of quantification, location, identification, tracking, and diagnosis, are described.

Description

[0001] Government License Rights [0002] The U.S. government has a fully paid-up license to this invention and in limited circumstances requires the patentee to license others on reasonable terms under Grant No. 1R43CA111752-01 issued by NIH / NCI and Grant No. 70NAB1H3028 issued by NIST . field of invention [0003] The present invention generally relates to submicron sized particles or labels immobilized covalently or non-covalently with entities of interest for quantification, localization, identification or tracking purposes. More specifically, the present invention relates to surface-enhanced spectroscopically active composite nanoparticles, methods of making the particles and uses of the particles. Background of the invention [0004] Fluorescence is the primary means of tracking and quantifying biomolecules. Fluorescent labels are used in DNA sequencing, gene expression analysis using microarrays, flow cytometry and its variants, RT-PCR and other application hosts. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75G01N33/20
CPCC25D1/00B82Y30/00B82Y5/00C25D1/003G01J3/06G01J3/10G01J3/44G01J2003/1213G01J2003/2826G01N21/658B82B1/00B82B3/00B82Y40/00
Inventor M·J·纳坦S·佩恩G·R·弗里曼G·查卡罗瓦W·E·多林I·沃尔顿
Owner BECTON DICKINSON & CO
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