Method for evaluating improvement of skin inflammation and aging based on zebra fish model and application thereof

Abstract

The invention discloses a method for evaluating improvement of skin inflammation and aging based on a zebra fish model and application thereof. The method comprises the following steps: S1, establishing a blank group; s2, establishing a model group; s3, establishing a sample group; s4, performing constant-temperature incubation; s5, observing the zebrafish of the blank group, the model group and the sample group under a body type fluorescence microscope, and recording the anti-inflammatory efficiency and the anti-wrinkle efficiency of the zebrafish; and S6, evaluating the skin inflammation and aging improving effect of the sample according to an anti-inflammation and anti-aging calculation formula. The method has the advantages of low cost, short test period, high reproducibility, high visualization degree, intuitive and understandable test result and the like, and can be used for accurately grading the efficacy of the cosmetic raw materials for improving skin inflammation and aging from the whole animal level.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetic efficacy evaluation, and in particular to a method for evaluating and improving skin inflammation and aging based on a zebrafish model and its application. Background Art

[0002] In skin inflammatory aging, inflammation and aging are mutually causal. When the skin is exposed to stressors for a long time, the dynamic balance between pro-inflammatory and anti-inflammatory is broken, leaving the skin in a state of chronic inflammation. Chronic inflammation leads to skin cell aging (such as DNA damage, telomere shortening, oxidative stress, etc.), which in turn accelerates the skin aging process. Aging skin cells secrete senescence-associated secretory phenotypes (SASPs), such as proinflammatory factors, chemokines, and matrix metalloproteinases, which change the skin microenvironment, affect the state of surrounding healthy cells, lead to DNA breakage and oxidative stress damage, and ultimately form a vicious cycle of inflammatory aging.

[0003] Repeated overexposure to UV radiation induces skin aging, a process that shares many similarities with natural aging. UV rays, particularly UVB, damage the DNA and protein structure of skin cells, leading to cellular aging. They also induce keratinocytes, dermal fibroblasts, and mast cells to secrete proinflammatory cytokines, triggering an inflammatory cascade in the skin. This creates a vicious cycle of inflammation and aging, ultimately leading to aging symptoms such as epidermal atrophy, delayed wound healing, and sagging.

[0004] Neutrophils are one of the most important innate immune cells involved in inflammatory responses. Activated by inflammatory stimuli, neutrophils migrate to inflamed areas of the skin under the influence of a gradient of soluble chemokines. They eliminate inflammation through phagocytosis, degranulation, and the production of reactive oxygen species and extracellular traps, thereby restoring homeostasis and promoting skin repair. If inflammation is not well regulated, persistent eosinophilic inflammation can cause skin tissue damage and chronic inflammation, leading to inflammatory aging of the skin and the appearance of aging phenotypes (such as wrinkles).

[0005] Existing inflammatory aging models are primarily cellular models. Single-cell models struggle to simulate the interactions between cells in vivo and fail to reflect true inflammatory responses in vivo. Zebrafish, a small vertebrate model organism, and transgenic zebrafish with labeled neutrophils provide a valuable tool for studying inflammatory responses in vivo. Their transparent larvae, small size, and robust reproductive capacity complement the shortcomings of traditional in vitro evaluation models, effectively reducing experimental costs and shortening experimental cycles while providing intuitive and easily interpretable results. Summary of the Invention

[0006] In vivo, UVB can activate neutrophils through the cell signal transduction system. Under the action of soluble chemokines, neutrophils are recruited to the skin inflammation reaction site, and participate in the intracellular inflammatory reaction process, remove related pathogens, and play an immunomodulatory role. Excessive UVB makes it difficult to effectively control inflammation. Sustained eosinophilic inflammation causes skin tissue damage and chronic inflammation, which then triggers skin inflammation and aging. Zebrafish, as a model organism, has a high genetic homology and skin structure similarity with humans, and this phenomenon is also present in its body. Therefore, the present invention uses zebrafish to establish a method for evaluating and improving skin inflammation and aging.

[0007] In a first aspect, the present invention provides a method for evaluating and improving skin inflammation and aging based on a zebrafish model, comprising the following steps:

[0008] Step S1, selecting several zebrafish with the same tail development status and not treating them to establish a blank group;

[0009] Step S2, selecting the same number of zebrafish with the same developmental status as in step S1, stimulating the zebrafish with UVB to establish a model group;

[0010] Step S3, selecting the same number of zebrafish as in step S1 and with the same developmental status, adding the test sample, and stimulating the zebrafish with UVB to establish a sample group;

[0011] Step S4, incubating the blank group, model group, and sample group under the same conditions for 16 h to 20 h;

[0012] Step S5, observing the zebrafish in the blank group, model group, and sample group under a stereo fluorescence microscope, and recording the anti-inflammatory efficiency and anti-wrinkle efficiency of the zebrafish;

[0013] Step S6: Evaluate the efficacy of the sample in improving skin inflammation and aging according to the anti-inflammatory aging calculation formula.

[0014] In the aforementioned zebrafish model-based evaluation of methods for improving skin inflammation and aging, the zebrafish used were 36-48 hpf (hpf) zebrafish Tg (mpx:dsRED) with red fluorescently labeled neutrophils. This zebrafish strain has a well-defined genome, high commercial availability, and stable embryo quality, which improves the stability of experimental results. Zebrafish neutrophils are generated from myeloid precursor cells and hematopoietic stem cells. The first mature neutrophils can be detected at 35 hpf, and all neutrophils are mature by 48 hpf. Zebrafish at 48 hpf are not yet capable of swimming, and UVB stimulation is more evenly distributed across the sides of the fish, resulting in a more uniform and stable modeling effect. Therefore, zebrafish Tg (mpx:dsRED) with red fluorescently labeled neutrophils at 36-48 hpf can be used as test organisms in this method.

[0015] In the above-mentioned zebrafish model-based method for evaluating the improvement of skin inflammation and aging, in steps S2 and S3, the UVB dose is 72mJ / cm 2 ~81mJ / cm 2 72mJ / cm 2 ~81mJ / cm 2 UVB stimulation caused neutrophils to migrate to the zebrafish skin and recruit to the inflamed areas. The inflammation was difficult to subside, resulting in a chronic inflammatory state in the zebrafish skin, which then triggered inflammatory aging of the skin. Specifically, the skin of the zebrafish caudal and dorsal fins showed obvious wrinkling and a large number of neutrophils were recruited to the wrinkles. This dose range was selected for the inflammatory aging model test. The fluorescence area of ​​the zebrafish caudal and dorsal fins increased, while the caudal and dorsal fin areas decreased in the blank and model groups. The relevant data showed significant differences, indicating that the inflammatory aging model was successfully established.

[0016] In the above-mentioned method for evaluating and improving skin inflammation and aging based on the zebrafish model, in step S2, the criteria for successfully establishing the model group are: compared with the blank group, the fluorescent area of ​​the caudal fin and dorsal fin of the model group increased, and the area of ​​the caudal fin and dorsal fin decreased; at the same time, the statistical data of the fluorescent area of ​​the caudal fin and dorsal fin of the blank group and the model group and the area of ​​the caudal fin and dorsal fin of the blank group and the model group were all less than 0.05 after variance analysis.

[0017] In the above method for evaluating the improvement of skin inflammation and aging based on the zebrafish model, in step S5, the anti-inflammatory efficiency R 抗炎 The observed indicator is the recruitment of neutrophils in the caudal fin and dorsal fin of zebrafish, that is, the fluorescent area of ​​the caudal fin and dorsal fin, and the calculation formula is as follows:

[0018]

[0019] Among them, S 样品组 is the average fluorescence area of ​​the caudal fin and dorsal fin of zebrafish in the sample group; S 模型组 is the average fluorescence area of ​​the caudal fin and dorsal fin of the model group; S 空白组 is the average fluorescence area of ​​the caudal fin and dorsal fin of the blank group.

[0020] In the above method for evaluating the improvement of skin inflammation and aging based on the zebrafish model, in step S5, the anti-wrinkle efficiency R 抗皱 The observation indicators are the areas of the zebrafish tail fin and dorsal fin, and the calculation formula is as follows:

[0021]

[0022] Among them, S 样品组 is the average area of ​​the caudal fin and dorsal fin of the zebrafish in the sample group; S 模型组is the average area of ​​the caudal fin and dorsal fin of the model group; S 空白组 is the average area of ​​the caudal fin and dorsal fin of the blank group.

[0023] In the above-mentioned method for evaluating and improving skin inflammation and aging based on the zebrafish model, in step S6, the anti-inflammatory aging calculation formula is:

[0024] R 抗炎症衰老 =0.4287*R 抗皱 +0.6315*R 抗炎 -1.368.

[0025] In a second aspect, the present invention provides the application of the above-mentioned method for evaluating the improvement of skin inflammation and aging based on the zebrafish model in screening raw materials for cosmetics with efficacy in improving skin inflammation and aging.

[0026] Compared with the prior art, the present invention has at least the following beneficial effects:

[0027] Most existing technologies use inflammatory aging cell models, which primarily evaluate the efficacy of samples in improving inflammatory aging at the molecular and cellular levels. These models are unable to simulate the interactions between cells in an organism and provide feedback on the true efficacy of the sample being tested. The present invention accurately grades the efficacy of cosmetic raw materials in improving skin inflammatory aging at the whole animal level, addressing the shortcomings of the existing technology. Furthermore, the present invention features simple operation, low cost, good reproducibility, and a high degree of visualization. It creatively uses a binary linear fitting equation to calculate the anti-inflammatory aging efficiency of a sample, which can simply and intuitively reflect the efficacy of cosmetic raw materials in improving skin inflammatory aging, providing scientific and intuitive data support for related product development by companies. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is a flow chart of the method for evaluating the improvement of skin inflammation and aging based on the zebrafish model of the present invention.

[0029] Figure 2 The phenotype diagram of zebrafish in the blank group, model group and sample group in Example 1.

[0030] Figure 3 The statistical results of the fluorescence areas of the zebrafish caudal fin and dorsal fin in the blank group, model group and sample group in Example 1 are shown.

[0031] Figure 4 The statistical results of the caudal fin and dorsal fin areas of zebrafish in the blank group, model group and sample group in Example 1.

[0032] Figure 5 The phenotype diagrams of zebrafish in the blank group and model group in Example 1 and Comparative Example 1 are shown.

[0033] Figure 6The caudal fin and dorsal fin area of ​​zebrafish in the blank group and model group in comparative example 1 ( Figure 6 Left), caudal fin and dorsal fin fluorescence area ( Figure 6 Right) Statistical results. DETAILED DESCRIPTION

[0034] The technical solution of the present invention will be further described below with reference to the accompanying drawings and through specific embodiments. However, the following examples are merely simplified examples of the present invention and do not represent or limit the scope of protection of the present invention. The scope of protection of the present invention shall be subject to the claims.

[0035] In the following examples, unless otherwise specified, all equipment and raw materials can be purchased from the market or are commonly used in the industry. The methods in the following examples, unless otherwise specified, are all conventional methods in the art.

[0036] The names of the samples to be tested in the following examples are shown in Table 1. The test samples were prepared by mixing a total flavonoid extract from Prinus utilis and decapeptide-4 in a mass ratio of 100:1. The total flavonoid extract from Prinus utilis was prepared according to the method described in Example 4 of Chinese invention patent CN108553527B, and the total flavonoid content of the extract was 56%. The decapeptide-4 was obtained from Hemei Biotechnology (Zhejiang) Co., Ltd. and had a purity of 95%. It is a cyclic peptide composed of ten amino acids with the sequence CIPRFYLFYC.

[0037] Table 1. Name of samples to be tested

[0038] Sample number Name of sample to be tested concentration Sample 1 Prinus utilis flavonoids combined with decapeptide-4 100 μg / mL Sample 2 Prinus utilis flavonoids combined with decapeptide-4 500 μg / mL Sample 3 Prinus utilis flavonoids combined with decapeptide-4 1.5 mg / mL Sample 4 Sodium lauryl sulfate 200 μg / mL Sample 5 Prinus utilis flavonoids combined with decapeptide-4 1mg / mL Sample 6 Prinus utilis flavonoids combined with decapeptide-4 300 μg / mL

[0039] <Example 1>

[0040] See also Figure 1 , a method for improving skin inflammation and aging based on a zebrafish model was evaluated, comprising the following steps:

[0041] Step S1: 30 36 hpf zebrafish (Tg(mpx:dsRED)) with normal development and consistent status were randomly selected and placed in a 12-well cell culture plate, with 10 zebrafish per well (2 mL per well). The standard dilution water in the 12-well cell culture plate was replaced without harming the juvenile fish, and no treatment was performed to establish a blank group.

[0042] Step S2: Select the same number of 36 hpf neutrophil red fluorescently labeled zebrafish Tg (mpx:dsRED) with the same developmental status as in step S1 and place them in a 12-well cell culture plate, with 10 fish per well and a volume of 2 mL per well. Replace the standard dilution water in the 12-well cell culture plate without harming the fry and use 72 mJ / cm 2 The zebrafish were stimulated with UVB to establish the model group;

[0043] Step S3: Select the same number of 36 hpf neutrophil red fluorescent labeled zebrafish Tg (mpx:dsRED) with the same developmental status as in step S1 and place them in a 12-well cell culture plate, with 10 fish per well and a volume of 2 mL per well. Replace the standard dilution water in the 12-well cell culture plate with the sample solution to be tested without harming the fry, and use 72 mJ / cm 2 UVB was used to stimulate zebrafish and establish sample groups;

[0044] Step S4, incubate the blank group, model group and sample group at 28.5°C for 20 h;

[0045] Step S5: After the incubation in step S4 is completed, observe and take pictures of each group of zebrafish under a stereo fluorescence microscope. The picture results are shown in Figure 2 The fluorescence area of ​​the caudal fin and dorsal fin, and the area of ​​the caudal fin and dorsal fin were statistically analyzed using ImageJ image processing software, and the anti-inflammatory efficiency (R 抗炎 ) and anti-wrinkle efficiency (R 抗皱 ):

[0046]

[0047] Among them, S 样品组 is the average fluorescence area of ​​the caudal fin and dorsal fin of zebrafish in the sample group; S 模型组 is the average fluorescence area of ​​the caudal fin and dorsal fin of the model group; S 空白组 is the average fluorescence area of ​​the caudal fin and dorsal fin of the blank group. 抗炎 ) is expressed as the relative fluorescence area of ​​the caudal fin and dorsal fin. Figure 3 . Figure 3 In the table, *** indicates that the data between the model group and the blank group are extremely significantly different, that is, P≤0.001; ### indicates that the data between the model group and the sample group are extremely significantly different, that is, P≤0.001.

[0048]

[0049] Among them, S 样品组 is the average area of ​​the caudal fin and dorsal fin of the zebrafish in the sample group; S 模型组 is the average area of ​​the caudal fin and dorsal fin of the model group; S 空白组 is the average area of ​​the caudal fin and dorsal fin of the blank group. The anti-wrinkle efficiency (R anti-wrinkle) is expressed by the relative area of ​​the caudal fin and dorsal fin. The results are shown in Figure 4 . Figure 4In the table, *** indicates that the data between the model group and the blank group are extremely significantly different, that is, P≤0.001; # indicates that the data between the model group and the sample group are significantly different, that is, 0.01<P≤0.05; ## indicates that the data between the model group and the sample group are very significantly different, that is, 0.001<P≤0.01; ### indicates that the data between the model group and the sample group are extremely significantly different, that is, P≤0.001.

[0050] Step S6, calculating the anti-inflammatory aging efficacy of the sample according to Formula 3:

[0051] R 抗炎症衰老 =0.4287*R 抗皱 +0.6315*R 抗炎 -1.368 (Formula 3).

[0052] Table 2. Results of efficacy evaluation on improving skin inflammation and aging

[0053]

[0054]

[0055] like Figure 2 As shown, the area within the dotted line is the area of ​​the zebrafish tail fin and dorsal fin ( Figure 2 Left), caudal fin and dorsal fin fluorescence area ( Figure 2 Right) statistical area. Combined Figure 3 and Figure 4 Compared with the blank control group, the caudal and dorsal fins of zebrafish in the model group showed significant wrinkling, and a large number of neutrophils were recruited to the wrinkled areas. Compared with the blank control group, the fluorescent area of ​​the caudal and dorsal fins in the model group increased, while the caudal and dorsal fin areas decreased. The data were analyzed by variance analysis with p values ​​less than 0.05, indicating that the inflammatory aging model was successfully established.

[0056] Compared with the model group, sample 1R 抗炎 >0% and p<0.05, indicating that sample 1 has anti-inflammatory effect; R 抗 Wrinkle < 0% and p > 0.05, indicating that the aging state of sample 1 is consistent with that of the model group and has no anti-wrinkle effect. From the phenotypic results, it can be seen that the degree of wrinkling of the tail fin and dorsal fin of sample 1 is similar to that of the model group, and neutrophils disappear in the wrinkled areas, indicating that sample 1 has a certain effect on improving skin inflammation and aging, which is consistent with R 抗炎症衰老 The calculated result is 25.78%.

[0057] Compared with the model group, sample 2R 抗炎 >0% and p<0.05, indicating that sample 2 has anti-inflammatory effect; R 抗Wrinkle>0% and p<0.05, indicating that sample 2 has an anti-wrinkle effect. From the phenotypic results, it can be seen that compared with the model group, the wrinkles of the tail fin and dorsal fin of sample 2 are less severe, and neutrophils show a significant disappearance in the wrinkled areas, indicating that sample 2 has a good effect in improving skin inflammation and aging, which is consistent with R 抗炎症衰老 The calculated results are consistent with 48.78%.

[0058] Compared with the model group, sample 3R 抗炎 >0% and p<0.05, indicating that sample 3 has anti-inflammatory effect; R 抗 Wrinkle>0% and p<0.05, indicating that sample 3 has an anti-wrinkle effect. From the phenotypic results, it can be seen that compared with the model group, the tail fin and dorsal fin of sample 3 zebrafish basically did not show wrinkles, and neutrophils appeared and significantly subsided in the tail fin and dorsal fin, indicating that sample 3 has a very good effect in improving skin inflammation and aging, which is consistent with R 抗炎症衰老 The calculated results are consistent with 102.45%.

[0059] Compared with the model group, sample 4R 抗炎 <0% and p < 0.05, indicating that sample 4 has a pro-inflammatory effect; R 抗 Wrinkle < 0% and p > 0.05, indicating that the aging state of sample 4 is consistent with that of the model group and has no anti-wrinkle effect. From the phenotypic results, it can be seen that compared with the model group, the degree of wrinkling of the tail fin and dorsal fin of sample 4 is similar to that of the model group, and neutrophils are more obviously recruited at the wrinkled areas, indicating that sample 4 has no effect on improving skin inflammation and aging, and has a pro-inflammatory effect, which is consistent with R 抗炎症衰老 The calculated result is -41.22%.

[0060] Compared with the model group, sample 5R 抗炎 >0% and p<0.05, indicating that sample 5 has anti-inflammatory effect; R 抗 Wrinkle>0% and p<0.05, indicating that sample 5 has an anti-wrinkle effect. From the phenotypic results, it can be seen that compared with the model group, the wrinkle degree of the zebrafish tail fin and dorsal fin in sample 5 was significantly reduced, and the neutrophils showed a significant disappearance in the wrinkled area, indicating that sample 5 has a good effect on improving skin inflammation and aging, which is consistent with R 抗炎症衰老 The calculated results are consistent with 64.24%.

[0061] Compared with the model group, sample 6R 抗炎 >0% and p<0.05, indicating that sample 6 has anti-inflammatory effect; R 抗 Wrinkle>0% and p>0.05, indicating that the aging state of sample 6 is consistent with that of the model group and has no anti-wrinkle effect. From the phenotypic results, it can be seen that compared with the model group, the degree of wrinkling of the tail fin and dorsal fin of sample 6 is similar to that of the model group, and neutrophils disappear in the wrinkled areas, indicating that sample 6 has a certain effect on improving skin inflammation and aging, which is consistent with R 抗炎症衰老The calculated result is 29.41%.

[0062] Comparative Example 1

[0063] The difference between Comparative Example 1 and Example 1 is that the UVB dose is 54mJ / cm 2 , the rest of the steps are exactly the same.

[0064] pass Figure 5 、 Figure 6 It can be seen that in Comparative Example 1, compared with the blank group, the fluorescent area of ​​the caudal fin and dorsal fin of the model group increased slightly, and the data were analyzed by variance analysis p < 0.05; the caudal fin and dorsal fin area decreased slightly, but the data were analyzed by variance analysis p = 0.1915 > 0.05. At the same time, compared with the model group in Example 1, the number of neutrophils in the caudal fin and dorsal fin wrinkles of Comparative Example 1 decreased significantly. The above results show that when the UVB dose is 54mJ / cm 2 Although neutrophils can be activated to migrate to the zebrafish skin, the inflammatory response is easy to subside and the skin damage is easy to repair, which cannot cause skin inflammation and aging, and ultimately leads to model failure. It is not suitable as a modeling dose.

[0065] Comparative Example 2

[0066] The difference between Comparative Example 2 and Example 1 is that the UVB dose is 108 mJ / cm 2 , the rest of the steps are exactly the same.

[0067] When the UVB dose is 108mJ / cm 2 The mortality rate of the model group was 53%, and subsequent efficacy evaluation was not possible. 2 When the dose is too high, it causes irreparable damage to the zebrafish and is not suitable as a modeling dose.

[0068] In summary, the present invention provides a method for evaluating and improving skin inflammation and aging based on a zebrafish model and its application, which accurately grades the efficacy of cosmetic raw materials in improving skin inflammation and aging based on the whole animal level. Different from traditional test models, the present invention is simple to operate, low in cost, reproducible, and highly visualized. It creatively uses a binary linear fitting equation to calculate the anti-inflammatory and aging efficiency of the sample, which can simply and intuitively reflect the efficacy of cosmetic raw materials in improving skin inflammation and aging, and provide scientific and intuitive data support for the development of related products of enterprises.

[0069] The applicant declares that the above is only a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Those skilled in the art should understand that any changes or substitutions that can be easily thought of by those skilled in the art within the technical scope disclosed by the present invention fall within the scope of protection and disclosure of the present invention.

Claims

1. A method for evaluating and improving skin inflammation and aging based on a zebrafish model, characterized in that: The following steps are involved: Step S1, selecting several zebrafish with the same tail development status and not treating them to establish a blank group; Step S2, selecting the same number of zebrafish with the same developmental status as in step S1, stimulating the zebrafish with UVB to establish a model group; Step S3, selecting the same number of zebrafish as in step S1 and with the same developmental status, adding the test sample, and stimulating the zebrafish with UVB to establish a sample group; Step S4, incubating the blank group, model group, and sample group under the same conditions for 16 h to 20 h; Step S5, observing the zebrafish in the blank group, model group, and sample group under a stereo fluorescence microscope, and recording the anti-inflammatory efficiency and anti-wrinkle efficiency of the zebrafish; Step S6: Evaluate the efficacy of the sample in improving skin inflammation and aging according to the anti-inflammatory aging calculation formula.

2. The method for evaluating and improving skin inflammation and aging based on a zebrafish model according to claim 1, characterized in that: The zebrafish is a neutrophil red fluorescence labeled zebrafish of 36 hpf to 48 hpf.

3. The method for evaluating and improving skin inflammation and aging based on a zebrafish model according to claim 1, characterized in that: In steps S2 and S3, the UVB dose is 72 mJ / cm 2 ~81mJ / cm 2 .

4. The method for evaluating and improving skin inflammation and aging based on a zebrafish model according to claim 1, wherein: In step S2, the criteria for determining whether the model group was successfully established are: compared with the blank group, the fluorescent area of ​​the caudal fin and dorsal fin of the model group increased, and the area of ​​the caudal fin and dorsal fin decreased; at the same time, the statistical data of the fluorescent area of ​​the caudal fin and dorsal fin of the blank group and the model group and the area of ​​the caudal fin and dorsal fin of the blank group and the model group were all less than 0.05 after variance analysis.

5. The method for evaluating and improving skin inflammation and aging based on a zebrafish model according to claim 1, characterized in that: In step S5, the anti-inflammatory efficiency R 抗炎 The observed indicator is the recruitment of neutrophils in the caudal fin and dorsal fin of zebrafish, that is, the fluorescent area of ​​the caudal fin and dorsal fin, and the calculation formula is as follows: Among them, S 样品组 is the average fluorescence area of ​​the caudal fin and dorsal fin of zebrafish in the sample group; S 模型组 is the average fluorescence area of ​​the caudal fin and dorsal fin of the model group; S 空白组 is the average fluorescence area of ​​the caudal fin and dorsal fin of the blank group.

6. The method for evaluating and improving skin inflammation and aging based on a zebrafish model according to claim 5, characterized in that: In step S5, the anti-wrinkle efficiency R 抗皱 The observation indicators are the areas of the zebrafish tail fin and dorsal fin, and the calculation formula is as follows: Among them, S 样品组 is the average area of ​​the caudal fin and dorsal fin of the zebrafish in the sample group; S 模型组 is the average area of ​​the caudal fin and dorsal fin of the model group; S 空白组 is the average area of ​​the caudal fin and dorsal fin of the blank group.

7. The method for evaluating and improving skin inflammation and aging based on a zebrafish model according to claim 6, characterized in that: In step S6, the anti-inflammatory aging calculation formula is as follows: R 抗炎症衰老 =0.4287*R 抗皱 +0.6315*R 抗炎 -1.368。 8. Use of the method for evaluating and improving skin inflammation and aging based on a zebrafish model according to any one of claims 1 to 7 in screening raw materials for cosmetics with efficacy in improving skin inflammation and aging.

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