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Antibacterial microemulsion gel and preparation method thereof

A technology of microemulsion gel and gelatin, which is applied in the field of biomedical engineering, can solve the problems of inability to achieve therapeutic purposes, lack of fine control of concentration, and low concentration of antibacterial agents, so as to improve resource utilization, reduce resistance to drug-resistant bacterial infections, The effect of good biocompatibility

Pending Publication Date: 2021-02-09
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these gels achieve the loading of natural plant essential oils, the release of essential oils comes from free diffusion, and the concentration lacks fine regulation, which may lead to the concentration of antibacterial agents being too low to achieve therapeutic purposes, making it difficult to really apply to complex clinical situations

Method used

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  • Antibacterial microemulsion gel and preparation method thereof
  • Antibacterial microemulsion gel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Dissolve 1.25g of B-type gelatin in 25mL of distilled water, stir at 50°C to form a gelatin solution, add 25mL of acetone, remove the supernatant, redissolve the precipitate in 15mL of distilled water and adjust the pH value to 12.0, add dropwise acetone again until A precipitate was generated, and then 250 μL of glutaraldehyde solution (25% aqueous solution) was added to the solution to continue the constant temperature reaction at 50° C. for 3 hours, and the reacted solution was refrigerated and centrifuged at 10,000 g for 30 minutes to obtain gelatin nanoparticles.

[0023] Take 8mL sodium periodate (0.5M) solution and add 10mL dextran aqueous solution (1g, 10%w / v, M w =60000), stirred at room temperature in the dark for 4 hours, and added 10 mL of ethylene glycol solution to terminate the reaction. Afterwards, the solution was fully dialyzed with deionized water and freeze-dried to obtain the oxidized polysaccharide.

[0024] Configure 2mL of continuous phase aqueo...

Embodiment 2

[0026] Dissolve 1.25g of B-type gelatin in 25mL of distilled water, stir at 50°C to form a gelatin solution, add 25mL of acetone, remove the supernatant, redissolve the white precipitate in 15mL of distilled water and adjust the pH value to 12.0, then add acetone dropwise again Until a white precipitate was produced, then 1.25 mL of genipin aqueous solution (10%) was added to the solution to continue the constant temperature reaction at 50° C. for 5 hours, and the reacted solution was refrigerated and centrifuged at 10,000 g for 30 minutes to obtain gelatin nanoparticles.

[0027] Take 8 mL of sodium periodate (0.5M) solution and add 10 mL of sodium hyaluronate aqueous solution (1 g, 10% w / v), stir at room temperature in the dark for 4 hours, and add 10 mL of ethylene glycol solution to terminate the reaction. Afterwards, the solution was fully dialyzed with deionized water and freeze-dried to obtain the oxidized polysaccharide.

[0028] Prepare 1 mL of continuous phase aqueou...

Embodiment 3

[0030]Dissolve 1.25g of B-type gelatin in 25mL of distilled water, stir at 50°C to form a gelatin solution, add 25mL of acetone, remove the supernatant, redissolve the precipitate in 15mL of distilled water and adjust the pH value to 12.0, add dropwise acetone again until A precipitate was generated, and then 625 μL of genipin aqueous solution (5%) was added to the solution to continue the constant temperature reaction at 50° C. for 10 hours, and the reacted solution was refrigerated and centrifuged at 10,000 g for 30 minutes to obtain gelatin nanoparticles.

[0031] Take 8mL sodium periodate (0.5M) solution and add 10mL sodium carboxymethylcellulose aqueous solution (1g, 10%w / v, M w =90000), stirred at room temperature in the dark for 4 hours, and added 10 mL of ethylene glycol solution to terminate the reaction. Afterwards, the solution was fully dialyzed with deionized water and freeze-dried to obtain the oxidized polysaccharide.

[0032] Configure 1.5mL of continuous phas...

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Abstract

The invention relates to antibacterial microemulsion gel and a preparation method thereof. The preparation method of the microemulsion gel takes a Pickering emulsion as a template, and comprises the following steps of (1) preparing a continuous phase which is composed of distilled water, gelatin nanoparticles, aminoglycoside antibiotics and oxidized polysaccharide; and (2) adding a dispersion phase containing antibacterial essential oil into the continuous phase, performing emulsifying to obtain the Pickering emulsion, and performing reaction crosslinking for a certain time to obtain the antibacterial microemulsion gel. The constructed microemulsion gel is intelligent microemulsion gel administrated as required, the release of the antibiotics and antibacterial essential oil can be regulated and controlled according to in-vitro environmental conditions, the antibacterial activity is high, and the microemulsion gel can be applied to the biomedical fields of drug controlled release systems, medical dressings and the like.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, in particular to an antibacterial microemulsion gel and a preparation method thereof. Background technique [0002] Bacterial infection is one of the most challenging problems in the medical field, and until today, bacterial infection is still a serious threat to human life. Aminoglycoside antibiotics are a class of broad-spectrum antibacterial drugs for the treatment of Gram-negative bacilli infections, and are listed by the World Health Organization as important antibacterial drugs for the treatment of tuberculosis, endocardial and meningeal infections. Although they have good clinical efficacy, aminoglycoside antibiotics usually suffer from adverse side effects, and, with the widespread use of antibacterial drugs in clinical practice, bacterial drug resistance is becoming more and more serious, making drug-resistant and multidrug-resistant infections a One of the most pressing public hea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/06A61K45/06A61K31/702A61K31/7036A61K31/7048A61K36/53A61K36/54A61K36/61A61K47/36A61K47/42A61P31/04
CPCA61K9/06A61K31/702A61K31/7036A61K31/7048A61K36/53A61K36/54A61K36/61A61K45/06A61K47/36A61K47/42A61P31/04A61K2300/00
Inventor 谭欢张瑞云韩立阳
Owner SOUTHWEST JIAOTONG UNIV
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