Reagent kit for real time fluorescence quantitative PCR detection of bacillus pyocyaneus

Abstract

The invention relates to a kit for detecting presence of pathogen pseudomonas aeruginosa in patient samples such as clinical bacterial pneumonia, corneal ulcer, urinary tract infection, septicemia and the like and samples such as cosmetics, environmental monitoring objects and the like, in particular to a kit for early and quickly diagnosing pseudomonas aeruginosa infection by a real-time fluorescence quantitative polymerase chain reaction technique.

Description

technical field [0001] The invention relates to a kit for detecting the presence of Pseudomonas aeruginosa (Pseudomonas aeruginosa) in samples from patients with clinical bacterial pneumonia, corneal ulcer, urinary tract infection, sepsis, etc. A kit for early and rapid diagnosis of Pseudomonas aeruginosa infection by real-time fluorescent quantitative polymerase chain reaction technology. Background technique [0002] Pseudomonas aeruginosa (PA), also known as Pseudomonas aeruginosa, belongs to the genus Pseudomonas and widely exists in nature. Infection is mainly manifested in burns, surgical trauma and postoperative infection. Clinically, it can cause sepsis, respiratory tract infection, endocarditis, urinary tract infection, central nervous system infection, bone and joint infection, ophthalmology, ear, mastoid and sinus infection, skin and soft tissue infection, and digestive tract infection. Because Pseudomonas aeruginosa is widely distributed, adaptable, and easy to...

AI Technical Summary

Problems solved by technology

Some laboratories at home and abroad have used PCR amplification target nucleic acid to detect Pseudomonas aeruginosa, such as: Kingsford detects Pseudomonas aeruginosa in wool eluate by amplifying 16S rRNA gene (Kingsford NM, Raadsma HW. Detection of Pseudomonas aeruginosa from ovine fleecewashings by PCR amplification of 16S ribosomal RNA[J].VeterinaryMicrobiol, 1995, 47(1-2): 1-70), Saint takes the lipoprotein I gene as a target, and detects green by designing primers and hybridization probes Pseudomonas (Saint O, Romeyer F, Lebel P, et al.Specificity of the Pseudomonas aeruginosa O1lipoprotein I gene as a DNA probe and PCR target region within the Pseudomonadaceae[J].JGeneral Microbiol,1992,138(4):733- 741.), Song detected Pseudomonas aeruginosa from ocular isolates by PCR using exotoxin A- specific primers. Mol Cell Probes2000; 14: 199-204.), due to the need for electrophoresis or hybridization analysis and other post-processing of PCR products, it is easy to cause contamination of PCR products and lead to false positives, so it is not suitable for clinical diagnosis

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