A kind of soybean rhizobia suitable for making freeze-dried powder and its application

A technology of soybean rhizobia and freeze-dried powder, which is applied in the direction of bacteria, microorganism-based methods, microorganisms, etc., can solve the problems of poor low-temperature resistance of rhizobia, achieve good low-temperature resistance, and facilitate low-temperature processing and storage.

Active Publication Date: 2022-05-06
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problem of poor low temperature resistance of the existing rhizobia, the present invention provides a soybean rhizobia suitable for making freeze-dried powder and its application

Method used

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  • A kind of soybean rhizobia suitable for making freeze-dried powder and its application
  • A kind of soybean rhizobia suitable for making freeze-dried powder and its application

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specific Embodiment approach 1

[0012] Specific embodiment one: this embodiment is suitable for making the soybean rhizobia of freeze-dried powder, which is bradyrhizobium japonicum (Bradyrhizobium japonicum) Bw 0122. CGMCC No. 16010.

[0013] The culture conditions of the slow-growing soybean rhizobium Bw 0122 in this embodiment are that the pH is 6.8-7.0, the temperature is 28-30°C, and the culture time is 3-5 days. The ratio consists of 10 parts of mannitol, 0.4 parts of yeast extract, 0.1 parts of sodium chloride, 0.5 parts of dipotassium hydrogen phosphate, 0.2 parts of magnesium sulfate, 18 parts of agar and 1000 parts of distilled water.

[0014] In this embodiment, the slow-growing soybean rhizobia is isolated and extracted from soybeans in the soybean field of Qiqihar City. Fresh and plump nodules are selected and cut off with scissors. First, the nodules are soaked in sterile water to remove impurities on the surface. After disinfecting the surface with absolute ethanol for 3 minutes, disinfect it...

specific Embodiment approach 2

[0018] Specific embodiment two: the application of soybean rhizobia as described in specific embodiment one as freeze-dried powder.

specific Embodiment approach 3

[0019] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the freeze-dried powder is prepared from Bradyrhizobium japonicum Bw 0122 through freeze-drying. Others are the same as in the second embodiment.

[0020] In this embodiment, the bradyrhizobium japonicum Bw 0122 is freeze-dried to make soybean rhizobium freeze-dried powder, wherein the freeze-drying method of soybean rhizobium (Bradyrhizobium japonicum) Bw 0122 is: use an ultracentrifuge to extract the bradyrhizobium japonicum Bradyrhizobium japonicum (Bradyrhizobium japonicum) Bw 0122 fermentation broth was continuously centrifuged at 10,000r / min for 5-10 minutes to remove the supernatant to collect the bacteria, add cryoprotectant to the bacteria at a ratio of 1:1 by weight, and shake evenly Bacterial suspension, the bacterial suspension was divided into 10ml ampoules, 3-5ml per bottle, pre-cooled in a refrigerator at 4°C for 30 minutes, then transferred to a freeze dryer at -20°C for pre-co...

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Abstract

A soybean rhizobia suitable for making freeze-dried powder and its application relates to a slow-growing soybean rhizobia and its application. The invention aims to solve the problem of poor low temperature resistance of the existing rhizobia. The soybean rhizobia suitable for making freeze-dried powder in the present invention is Bradyrhizobium japonicum Bw 0122, which can be used as freeze-dried powder. The slow-growing soybean rhizobia Bw 0122 of the present invention has no significant decrease in the number of viable bacteria after freezing treatment, and has excellent freeze-drying activity.

Description

technical field [0001] The invention relates to a brady-growing soybean rhizobia and application thereof. Background technique [0002] Through the symbiotic nitrogen fixation of rhizobia, leguminous plants can convert molecular nitrogen in the air into ammonia nitrogen for use by plants. A small root nodule is a miniature nitrogen fertilizer factory. The inoculation of rhizobia in leguminous crops can increase the yield, especially the inoculation of rhizobia in soybeans, peanuts and other crops has become a conventional technology in agricultural production. The use of rhizobia agents is beneficial to increase the number, quality and nitrogen fixation of legume root nodules, can effectively reduce the use of chemical fertilizers, improve soil and ecological environment quality, increase crop yield and quality, and is conducive to the adjustment of agricultural industrial structure. In short, the research and application of rhizobia will play an important role in increasin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/01
Inventor 安琦原韬曹亚彬殷博牛彦波吴皓琼张超
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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