Method for promoting plants to restore soil polluted by nickel through vesicularis pseudo unit cell bacterium

A technology of Pseudomonas vesicularis and phytoremediation, applied in the restoration of contaminated soil, etc., can solve the problems of small biomass, limited soil purification efficiency, and slow growth, so as to increase plant biomass and improve green In situ repair effect, effect of phytoremediation promotion

Active Publication Date: 2005-11-16
SUN YAT SEN UNIV
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Problems solved by technology

[0006] Although some successful cases have been achieved in the treatment of heavy metal-contaminated soils by using hyperaccumulative plants, most of these plants grow slowly and have low biomass due to the mutants produced under long-term

Method used

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Examples

Experimental program
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Embodiment

[0031] Example: Soil Remediation

[0032] (1) Seedling preparation: the test plant is a nickel hyperaccumulator plant—Alyssum corsicum, and there are 2 groups of bacterial separation treatment and control. The seeds were sterilized with 70% ethanol for 1 min, soaked in 1% sodium hypochlorite for 10 min, and then rinsed with sterile water (generally 3 times); 600 0.8~1.5) Soak for 1h. Seeds used for control were soaked directly in deionized water. Then transfer to nutrient soil to germinate and raise seedlings, and transplant after the seedlings grow to 4-5 leaves.

[0033] (2) Test soil: Zhuyu type paddy soil, Yunnan Yuanjiang soil and Yunnan Mojiang soil; soil heavy metal nickel content: paddy soil > 400mg / kg, Yunnan Yuanjiang soil > 3500mg / kg, Yunnan Mojiang soil > 5400mg / kg.

[0034] (3) Transplanting and management: Before plant transplanting, apply bacterial solution (OD 600 0.8~1.5) 20mL; when transplanting seedlings, dip the plant root system in the bacterial soluti...

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PUM

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Abstract

A method using pseudomanas vesicularis to promote plant to repair the soil polluted by Ni features that said pseudomanas vesicularis, which can secrete Fe arrier and decrease the content of ethene in plant, is inoculated to the root of plant growing in said polluted soil for increasing the effect of plant to absorb the Ni, so repairing the soil.

Description

technical field [0001] The invention relates to a method for promoting phytoremediation of soil nickel pollution by utilizing Pseudomonas vesicularis. Background technique [0002] Heavy metal pollution in soil has a wide range of sources and various forms. For example, waste water, waste gas and waste residues discharged in the process of industrial production, as well as chemical fertilizers, pesticides and herbicides used in large quantities in agricultural production. When the amount of these pollutants absorbed by the soil exceeds its own purification capacity, it leads to soil pollution. According to statistics, millions of tons of toxic heavy metals are released into the environment every year around the world, of which 381,000 tons are nickel. By 1998, the area of ​​my country's farmland polluted by heavy metals had reached 20 million hectares, which had a non-negligible impact on agricultural production, environmental safety and human health. [0003] At present,...

Claims

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Application Information

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IPC IPC(8): B09C1/10
Inventor 仇荣亮蔡信德
Owner SUN YAT SEN UNIV
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