Method for promoting plants to restore soil polluted by nickel through vesicularis pseudo unit cell bacterium
A technology of Pseudomonas vesicularis and phytoremediation, applied in the restoration of contaminated soil, etc., can solve the problems of small biomass, limited soil purification efficiency, and slow growth, so as to increase plant biomass and improve green In situ repair effect, effect of phytoremediation promotion
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[0031] Example: Soil Remediation
[0032] (1) Seedling preparation: the test plant is a nickel hyperaccumulator plant—Alyssum corsicum, and there are 2 groups of bacterial separation treatment and control. The seeds were sterilized with 70% ethanol for 1 min, soaked in 1% sodium hypochlorite for 10 min, and then rinsed with sterile water (generally 3 times); 600 0.8~1.5) Soak for 1h. Seeds used for control were soaked directly in deionized water. Then transfer to nutrient soil to germinate and raise seedlings, and transplant after the seedlings grow to 4-5 leaves.
[0033] (2) Test soil: Zhuyu type paddy soil, Yunnan Yuanjiang soil and Yunnan Mojiang soil; soil heavy metal nickel content: paddy soil > 400mg / kg, Yunnan Yuanjiang soil > 3500mg / kg, Yunnan Mojiang soil > 5400mg / kg.
[0034] (3) Transplanting and management: Before plant transplanting, apply bacterial solution (OD 600 0.8~1.5) 20mL; when transplanting seedlings, dip the plant root system in the bacterial soluti...
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