Plant growth-promoting rhizobacteria and application thereof

A technology for plant roots and growth-promoting bacteria, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as soil environment that may not be suitable for fluvo-aquic soil, achieve good growth-promoting effects, improve utilization, increase Effects of Phosphorus Content in Plants

Active Publication Date: 2013-06-12
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the plant growth-promoting bacteria are still domesticated into bacteria that can be effectively used to promote plant growth and have better nitrogen fixation and phosphorus dissolution effects, and may not be suitable for the soil environment of fluvo-aquic soil.

Method used

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  • Plant growth-promoting rhizobacteria and application thereof
  • Plant growth-promoting rhizobacteria and application thereof
  • Plant growth-promoting rhizobacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] First prepare the following media.

[0034] LB medium: peptone 10g, yeast extract 5g, sodium chloride 10g, agar 20g, distilled water 1000ml, pH7.0-7.2, sterilized at 121°C for 20min.

[0035] LB liquid medium: without agar, other conditions are the same as above.

[0036] Inorganic phosphorus bacteria medium (PKO medium): 5g tricalcium phosphate, 10g glucose, 0.5g ammonium sulfate, 0.3g sodium chloride, 0.3g magnesium sulfate heptahydrate, 0.3g potassium chloride, 0.03g manganese sulfate, heptahydrate Ferrous sulfate 0.03g, pH7.0 agar 20g, distilled water 1000ml, pH7.0~7.2, sterilized at 121°C for 20min.

[0037] Weigh 10g of the plant rhizosphere fluvo-aquic soil collected from Banqiao, Nanjing, and put it in a 250ml Erlenmeyer flask filled with 100ml of sterilized water. In a shaker, shake at 30°C and 150r / min for 20min. Set aside for 10 minutes to obtain a soil suspension. The soil suspension contains several kinds of growth-promoting bacteria, which are diluted b...

Embodiment 2

[0045] Aerobic test

[0046] Pour the sterilized LB culture medium into 3 sterilized test tubes, at about 2 / 3, on the aseptic operating table, use an inoculation needle to pick up the bacteria cultured on the slant, puncture and inoculate into the above culture base (must pierce to the bottom of the tube). Cultivate at 30°C, and observe the results in 3 days to 7 days respectively. Those that grow on the surface of the agar column are aerobic bacteria, and those that grow along the puncture line are anaerobic or facultative anaerobic bacteria.

[0047] The test results of plant rhizosphere growth-promoting bacteria Y1 (CGMCC No.7130) showed that colonies grew along the surface of the agar column, and no colonies grew in the puncture line, which was strictly aerobic.

[0048] Determination of catalase

[0049] Put 1 drop of 3% H on a clean slide 2 o 2 , take 1 ring of 18-24h LB slant culture, in H 2 o 2 Smear in the middle, if bubbles are produced, it is positive, otherw...

Embodiment 3

[0076] In order to further verify the ability and optimum conditions of the rhizosphere growth-promoting bacteria Y1 (CGMCC No.7130) obtained in Example 1 to decompose phosphorus, the effect of phosphorus solubilization on different pH, different liquid volumes, different carbon sources, and different nitrogen sources is as follows Impact.

[0077] Adjust the inorganic phosphorus bacteria medium (PKO medium) to different pH (4, 5, 6, 7, 8, 9, 10), take 50mL and put it in a 250mL Erlenmeyer flask, press 1% (v / v ) inoculum amount to inoculate the Y1 bacteria in the logarithmic growth phase, place it at 30°C, and culture it on a shaker at 180r / min for 96 hours, and measure the phosphorus-dissolving effect of the Y1 bacteria according to the method of quantitative determination. The results are as follows: figure 2 As shown, it shows that Y1 (CGMCC No.7130) has a poor phosphorus-solubilizing effect when the pH is 9 and 10. In a strong alkaline environment, the bacteria cannot gro...

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Abstract

The invention belongs to the field of agricultural microbiology, and relates to plant growth-promoting rhizobacteria and an application thereof. The plant growth-promoting rhizobacteria (Pseudomonas fluorescens Y1) was collected in the General Microbiological Center of China Microbiological Culture Collection Management Committee on January 11th, 2013, and is named Pseudomonas fluorescens in a classified manner, and the collection number is CGMCC (China General Microbiological Culture Collection Center) NO. 7130. The strain utilizes insoluble phosphate as a phosphorus source for strong growth ability, can improve the utilization ratio of the fertilizer, promotes the development of plant roots and uptake of a fertilizer and increases the phosphorus content of the plant. The plant growth-promoting rhizobacteria disclosed by the invention has a good growth promoting effect for peanuts, and the efficient phosphate solubilisation of the strain promotes the growth and the development of the peanuts and the utilization ratio of a phosphorus fertilizer.

Description

technical field [0001] The invention belongs to the field of agricultural microorganisms, and relates to a rhizosphere growth-promoting bacterium and its application. Background technique [0002] Fluvo-aquic soil is the soil formed by river sediments affected by groundwater movement and farming activities. It is named after the night tide phenomenon. In China, it is mostly distributed in the alluvial plains of the middle and lower reaches of the Yellow River, the plains of Jiangsu and Anhui to the south, and the river, lake plains and deltas of the middle and lower reaches of the Yangtze River Basin. The distribution area of ​​fluvo-aquic soil is flat, with deep soil layer, abundant water and heat resources, and wide variety of seed production. It is the main dryland soil in my country and is rich in grain and cotton. However, the Huang-Huai-Hai Plain, where the fluvo-aquic soil is the largest, suffers from frequent droughts and floods, as well as saline-alkali hazards. In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C09K17/14C12R1/39C09K101/00
Inventor 李辉信虞丽闫小梅叶成龙姜瑛胡锋焦加国徐莉刘满强陈小云
Owner NANJING AGRICULTURAL UNIVERSITY
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