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Method for improving cadmium resistance of tomatoes

A technology of tomato and cadmium resistance, applied in the fields of land preparation methods, horticultural methods, botanical equipment and methods, etc., can solve the problem of less research on synergistic promotion, and achieve the effect of reducing potential risks, improving cadmium resistance and strong cadmium resistance.

Inactive Publication Date: 2018-10-02
SUN YAT SEN UNIV
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Problems solved by technology

[0005] The synergistic effect of PGPR and AMF on plant growth has been widely concerned, but there are still few studies on the synergistic promotion of plant growth and heavy metal tolerance under heavy metal stress

Method used

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  • Method for improving cadmium resistance of tomatoes
  • Method for improving cadmium resistance of tomatoes
  • Method for improving cadmium resistance of tomatoes

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Embodiment 1

[0032] The preparation process of the tested bacterial agent: Soil samples were collected from the rhizosphere of many common crops polluted by acid mine wastewater in Shangba Village, Guangdong Province. The soil suspension was inoculated into LB solid medium (10.0 g of bacteriological peptone, 5.0 g of yeast extract, 10.0 g of NaCl, 1 L of deionized water, and 1 L of agar) added with 30 mg / L Cd(II) by dilution coating 15g, pH 7.0), pick a single colony of bacteria with different shapes and plump growth on the plate, and streak on the solid medium containing Cd(II) (30mg / L), enrich and purify continuously for 2-3 times, and then Obtain pure cultures of Cd-tolerant bacteria. After separation and purification, the test strain was spread on the LB solid medium plate, and incubated at 30°C for 48 hours, and a single colony with good separation was selected and sent to the biological company for 16SrRNA strain identification. Two strains of bacteria PGPR (Enterobacter sp. (herein...

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Abstract

The invention provides a method for improving cadmium resistance of tomatoes. The method includes the steps: 1) transplanting tomato seedlings into cadmium contaminated soil and inoculating AMF (arbuscular mycorrhizal fungi) bacteria; 2) inoculating plant growth promoting rhizobacteria to a plant root system; 3) culturing the tomatoes to be mature and generate fruits. By co-inoculation of the plant growth promoting rhizobacteria and arbuscular mycorrhizal fungi, growth of the tomatoes is promoted under cadmium stress, the content of heavy metals in plants can be reduced without affecting soilproperties, directions are provided for research of combined effects of the plant growth promoting rhizobacteria and the AMF on plant growth and metal resistance, and routes are provided for exploringthe action mechanism of the plant growth promoting rhizobacteria and the AMF.

Description

technical field [0001] The invention belongs to the technical field of remediation of heavy metal pollution, and in particular relates to a method for improving tomato cadmium resistance, in particular to a method for promoting the growth of tomato plants under cadmium stress by using plant rhizosphere growth-promoting bacteria and Glomus moesei. Background technique [0002] Heavy metal pollution in farmland is becoming more and more serious, which can inhibit the growth of crops and reduce crop yield, and will also seriously threaten human health. In order to improve the status quo of heavy metal pollution in farmland, phytoremediation technology is widely used, and phytobarrier has become one of the important remediation technologies to improve the safety of agricultural products. Improving crop growth by adding chemical modifiers or microbial assistance has achieved certain results, but the addition of chemical modifiers will affect soil fertility and structure or have t...

Claims

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Application Information

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IPC IPC(8): A01G22/05A01B79/00A01G7/06A01N63/04A01P21/00
CPCA01B79/00A01G7/06A01G22/05A01N63/30
Inventor 王诗忠李元媛曾加会黄礼格阮迪申仇荣亮晁元卿汤叶涛
Owner SUN YAT SEN UNIV
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