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Gene improving cadmium tolerance of plant and application thereof

A cadmium, plant-resistant technology, applied in the field of bioengineering, can solve problems such as damage to the normal function of the body, soil, water source damage, etc.

Inactive Publication Date: 2013-11-06
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pollution caused by these heavy metals will not only cause serious harm to soil and water sources; once it enters the organism, it will greatly damage the normal function of the organism

Method used

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  • Gene improving cadmium tolerance of plant and application thereof
  • Gene improving cadmium tolerance of plant and application thereof
  • Gene improving cadmium tolerance of plant and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1, the acquisition of XCD1 and its coding gene

[0034] Using chemical induction to activate the XVE (LexA-VP16-ER) mutant system (provided by the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences), from containing 100 μM CdCl 2 The Arabidopsis seedlings screened in the 1 / 2MS medium were used as materials ( figure 1 ), use the mutant genomic DNA extracted by the classic CTAB method as a template, and perform the following Tail-PCR reaction (Yao-Guang Liu et al., 1995):

[0035] Tail-PCR reaction program (including three rounds of reaction, Table 1):

[0036]

[0037]

[0038] Table 1 Tail-PCR reaction program

[0039]

[0040] Among them, nested primers: LexA2, LexA3, LexA4, LexA5, LexA6, and random primers: AD1, AD2, AD3, AD4 are shown in Table 2.

[0041] Table 2 Primer sequences used in Tail-PCR

[0042]

[0043]

[0044] Perform 1% agarose gel electrophoresis on the third-round product of Tail-PCR. The obtained ...

Embodiment 2

[0045] Embodiment 2, cultivate cadmium-tolerant Arabidopsis thaliana

[0046] 1. Obtaining and phenotypic analysis of cadmium-tolerant mutant xcd1

[0047] The Arabidopsis mutant library of independent transformation lines constructed by chemically inducing and activating the XVE (LexA-VP16-ER) mutant system, under the stress condition of 100 μM CdCl2 and the induction of 10 μM external hormone β-estradiol, at 1 / The WT and mutants were sowed in 2MS medium and cultured horizontally for 1w, and several cadmium-tolerant mutants were screened ( figure 1 ), the larger seedlings were selected for follow-up research, and the gain-of-function cadmium-tolerant mutant xcd1 was obtained through phenotypic identification (Fig. 2A-C).

[0048] 2. Comparison of cadmium accumulation between xcd1 and wild-type plants

[0049] Since the induction and activation of XCD1 will significantly enhance the plant's tolerance to heavy metal Cd, whether the Cd content in the mutant also changes. Ther...

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Abstract

The invention discloses a plant cadmium-tolerant protein which has cadmium tolerance and enhances the cadmium absorption of a plant and a coding gene thereof. The invention is characterized in that the amino acid sequence of the protein is as shown in SEQ ID NO:2 in a sequence table; and the nucleotide sequence is as shown in SEQ ID NO:1. According to the invention, the expression of the cadmium-tolerant protein coding gene induced or transferred into a plant enhances the cadmium tolerance of the plant; and thus, plants provided with the genes which are cloned and recombined by the transgenic technology can be grown in a cadmium-polluted environment to restore the soil environment.

Description

1. Technical field [0001] The invention relates to the field of bioengineering, in particular to a method for improving plant cadmium resistance-related protein and its coding gene and application, in particular to a method for using the gene to increase plant tolerance to cadmium poisoning. 2. Background technology [0002] In recent years, due to the unprecedented expansion of industrialization, a large amount of mineral energy and resources have been developed, so that these heavy metals originally fixed in rocks can be released in large quantities into the space accessible to life activities. The pollution caused by these heavy metals will not only cause serious harm to soil and water sources; once it enters the organism, it will greatly damage the normal function of the organism. Water and soil pollution caused by heavy metals has posed a serious threat to the global ecological environment, food safety, human health and sustainable agricultural development. Therefore, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N5/10C12N1/15C12N1/19C12N1/21A01H5/00
Inventor 曹树青王敏柏晓娅孙泽华阳立波
Owner HEFEI UNIV OF TECH
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