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A method to improve tomato cadmium tolerance

A tomato and capacity technology, applied in the field of improving tomato cadmium tolerance, can solve the problem of less synergistic promotion research

Inactive Publication Date: 2021-04-27
SUN YAT SEN UNIV
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Problems solved by technology

[0005] The synergistic effect of PGPR and AMF on plant growth has been widely concerned, but there are still few studies on the synergistic promotion of plant growth and heavy metal tolerance under heavy metal stress

Method used

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  • A method to improve tomato cadmium tolerance
  • A method to improve tomato cadmium tolerance
  • A method to improve tomato cadmium tolerance

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Embodiment 1

[0032] The preparation process of the tested bacterial agent: Soil samples were collected from the rhizosphere of many common crops polluted by acid mine wastewater in Shangba Village, Guangdong Province. The soil suspension was inoculated into LB solid medium (10.0 g of bacteriological peptone, 5.0 g of yeast extract, 10.0 g of NaCl, 1 L of deionized water, and 1 L of agar) added with 30 mg / L Cd(II) by dilution coating 15g, pH 7.0), pick a single colony of bacteria with different shapes and plump growth on the plate, and streak on the solid medium containing Cd(II) (30mg / L), enrich and purify continuously for 2-3 times, and then Obtain pure cultures of Cd-tolerant bacteria. After separation and purification, the test strain was spread on the LB solid medium plate, and incubated at 30°C for 48 hours, and a single colony with good separation was selected and sent to the biological company for 16SrRNA strain identification. Two strains of bacteria PGPR (Enterobacter sp. (herein...

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Abstract

The invention provides a method for improving tomato cadmium tolerance, comprising the following steps: 1) transplanting tomato seedlings into cadmium-contaminated soil and simultaneously inoculating AMF bacterial agent; 2) after the tomato seedlings grow stably, inoculating plant roots 3) Cultivate tomato until it matures and produces fruit. The invention promotes the growth of tomato under cadmium stress by co-inoculating plant rhizosphere growth-promoting bacteria and arbuscular mycorrhizal fungi, which can not only reduce the content of heavy metals in plants, but also not affect the properties of soil, and provide a booster for plant rhizosphere. The joint action of bacteria and AMF provides a direction for the research on plant growth and metal tolerance, and provides a route for exploring the mechanism of action of the two.

Description

technical field [0001] The invention belongs to the technical field of remediation of heavy metal pollution, and in particular relates to a method for improving tomato cadmium resistance, in particular to a method for promoting the growth of tomato plants under cadmium stress by using plant rhizosphere growth-promoting bacteria and Glomus moesei. Background technique [0002] Heavy metal pollution in farmland is becoming more and more serious, which can inhibit the growth of crops and reduce crop yield, and will also seriously threaten human health. In order to improve the status quo of heavy metal pollution in farmland, phytoremediation technology is widely used, and phytobarrier has become one of the important remediation technologies to improve the safety of agricultural products. Improving crop growth by adding chemical modifiers or microbial assistance has achieved certain results, but the addition of chemical modifiers will affect soil fertility and structure or have t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/05A01B79/00A01G7/06A01N63/30A01N63/20A01P21/00
CPCA01B79/00A01G7/06A01G22/05A01N63/30
Inventor 王诗忠李元媛曾加会黄礼格阮迪申仇荣亮晁元卿汤叶涛
Owner SUN YAT SEN UNIV
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