Ardisia japonica phyllobacterium RC6b and application of same in soil remediation

A technology of Pyrobacterium bovis and contaminated soil, which is applied in the restoration of contaminated soil, bacteria, and microbial-based methods, can solve the problems of no microbial resources, etc., and achieve the improvement of phytoremediation efficiency, strong heavy metal resistance and adsorption ability, growth-promoting effect

Active Publication Date: 2013-02-06
中科华鲁土壤修复工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no microbial resources capable of simultaneously adsorbing several heavy metals have been found.

Method used

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  • Ardisia japonica phyllobacterium RC6b and application of same in soil remediation
  • Ardisia japonica phyllobacterium RC6b and application of same in soil remediation
  • Ardisia japonica phyllobacterium RC6b and application of same in soil remediation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022]Example 1: Isolation, identification and characteristics of Phyllobacterium myrsinacearum RC6b CGMCC No.6621

[0023] 1.1 Test soil

[0024] The rhizosphere soil collected from a zinc-cadmium hyperaccumulator plant Sedum sedatus growing in a lead-zinc mining area in Zhejiang. Its basic physical and chemical properties are: pH 7.6, organic matter 13.6g kg -1 , total amount of copper 1826.7mg kg -1 , the total amount of zinc is 991.9mg kg -1 , the total amount of cadmium is 91.3mg kg -1 , the total amount of lead is 14207.4mg kg -1 . Fresh soil samples were passed through a 2mm sieve and stored in the dark at 4°C.

[0025] 1.2 Heavy metals for testing

[0026] CdCl 2 2.5H 2 O, ZnSO 4 ·7H 2 O and Pb(NO 3 ) 2 All were purchased from Sigma Company (USA), and were of analytical grade.

[0027] 1.3 Culture medium type

[0028] ①Luria-Bertani (LB) medium: containing 5g yeast extract, 10g peptone, 10gNaCl per liter; ②The growth-promoting bacteria isolation medium w...

Embodiment 2

[0055] Example 2: Bioremediation effect of Phyllobacterium myrsinacearum RC6b CGMCC No.6621

[0056] 1.1 Test soil

[0057] Collected from a heavy metal polluted farmland soil in Zhejiang. The basic physical and chemical properties of the soil are: pH 7.3, organic matter 16.0g kg -1 , total amount of copper 203.0mg kg -1 , the total amount of zinc is 736.2mg kg -1 , the total amount of cadmium is 5.9mg kg -1 , the total amount of lead is 153.3mg kg -1 . Fresh soil samples were passed through a 2mm sieve and stored in the dark at 4°C.

[0058] 1.2 Culture medium type

[0059] ①Luria-Bertani (LB) medium: containing 5g yeast extract, 10g peptone, 10gNaCl per liter; ②The growth-promoting bacteria isolation medium with ACC deaminase activity, that is, ADF medium (1L); Dworkin and Foster ( DF) stock solution plus ACC (final concentration is 3nmol L -1 ) as the only nitrogen source medium, agar 20g, pH 7.2. DF mother liquor contains 4g KH per liter 2 PO 4 ,6g Na 2 HPO 4...

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Abstract

The invention provides ardisia japonica phyllobacterium RC6b and application of the ardisia japonica phyllobacterium RC6b in soil remediation. The strain is stored in a depository authority specified by State Intellectual Property Office with a number of CGMCC No. 6621 on September 25, 2012; and the depository authority is a common microbe center of China Committee for Culture Collection of Microorganisms. The plant growth promoting rhizobacteria is high in heavy metal resistance and absorbing performance, can obviously promote t growth of a plant, reduce toxicity of heavy metal to plant, and improve phyto-availability of the heavy metal in soil, so that the ardisia japonica phyllobacterium RC6b can be directly adopted for improving plant repair efficiency; and the ardisia japonica phyllobacterium RC6b has a good application prospect in bioremediation of the soil polluted by the heavy metal.

Description

1. Technical field [0001] The present invention relates to Pyrobacterium bovis, in particular to a plant growth promoting rhizobacteria (PGPR) Pyrobacterium bovis RC6b and its application in remediation of heavy metal polluted soil. 2. Background technology [0002] Heavy metals are a kind of inorganic pollutants that are ubiquitous in the environment. Because they are relatively stable and cannot be degraded in the environment, it is very difficult to treat heavy metal pollution. In particular, heavy metal pollution in soil not only affects the yield and quality of crops, but also pollutes groundwater and poses a great threat to human health through the "biological amplification" of the food chain. Repairing heavy metal-contaminated soil and restoring its original functions has always been a hot and difficult point in international research. At present, there are two main ways to remediate heavy metal pollution: 1. Change the state of heavy metals, solidify or passivate th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
Inventor 马莹骆永明滕应李振高
Owner 中科华鲁土壤修复工程有限公司
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