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Method for promoting plants to restore soil hardening through utilizing Acetobacter xylinum

Acetobacter xylinum and phytoremediation technology, applied in the agricultural field, can solve problems such as energy consumption, weakened respiration, insufficient energy supply, and impact on absorption, achieving considerable economic benefits, increasing plant biomass, and improving absorption.

Inactive Publication Date: 2013-01-02
崔青青
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of soil compaction or long-term flooding, the respiration of plant root cells is weakened, and nutrients such as nitrogen exist in an ion state, and the absorption is mostly in the form of active transport, which consumes the energy generated by cell metabolism. The respiration is weakened, so the energy supply Insufficient, affecting absorption

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of seedlings: the plants to be tested are corn, the seeds of the gramineous herbaceous plant maize; the seeds are sterilized with 70% ethanol for 1 min, soaked in 1% sodium hypochlorite for 10 min, and then rinsed with sterile water (generally rinsed 3 times); 5mL bacterial solution (OD 600 0.8) Soak for 1h;

[0026] 2. the preparation method of described Acetobacter xylinum bacterium liquid is: adopt plant rhizosphere soil to prepare soil suspension, soil suspension is planted to PAF substratum (peptone 10g, tyrosine 10g, MgSO 4 1.5g, H 2 PO 4 1.5g, glycerin 10mL, H 2 (01000mL, pH7.0), after cultivating at 100r / min25°C for 5d, apply 0.5mL Acetobacter xylinum on the solid plane culture medium, put it in the incubator and cultivate it at 37°C, after the growth of colonies on the plane culture medium, The colonies were planted on a new PAF solid medium for cultivation, and after two separations, the purified Acetobacter xylinum strain was obtained, and...

Embodiment 2

[0030] 1. Preparation of seedlings: the plants to be tested are corn, the seeds of the gramineous herbaceous plant maize; the seeds are sterilized with 70% ethanol for 1 min, soaked in 1% sodium hypochlorite for 10 min, and then rinsed with sterile water (generally rinsed 3 times); 5mL bacterial solution (OD 600 0.8) Soak for 1h;

[0031] 2. the preparation method of described Acetobacter xylinum bacterium liquid is: adopt plant rhizosphere soil to prepare soil suspension, soil suspension is planted to PAF substratum (peptone 10g, tyrosine 10g, MgSO 4 1.5g, H 2 PO 4 1.5g, glycerol 10mL, H 2 (O 1000mL, pH7.0), after culturing at 100r / min25°C for 5 days, spread 0.5mL of Acetobacter xylinum on the solid plane medium, put it in the incubator at 37°C, and wait until the colonies grow on the plane medium , the colonies were planted on a new PAF solid medium for cultivation, and after 2 separations, the purified Acetobacter xylinum strain was obtained, and then transferred to th...

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Abstract

The invention relates to a method for promoting plants to restore soil hardening through utilizing Acetobacter xylinum, and belongs to the technical field of the agriculture. The method is characterized in that the method comprises the following steps: carrying out expanding culture of Acetobacter xylinum strains to prepare a bacterial liquid, wherein the concentration of the bacterial liquid, represented by OD600, is not less than 0.8 (the OD600 is generally between 0.8 and 1.5); controlling the pH value of soil at 5.5-7; and processing the soil restoration plants by the bacterial liquid. The method has the following beneficial effects: the Acetobacter xylinum selected in the invention can promote the growth of the plants and improve the biomass of the plants through the symbiotic relation between rhizosphere bacteria and the plants, so the rich water absorption of the plants and the hardened soil restoration effect are improved, and potential economic effects are apparent; and the used strains improve present green in-situ restoration effects, and gained plant overground parts can generate economic effects and also can improve the soil degeneration and the productivity decrease caused by the soil pollution of fertilizers and the like.

Description

technical field [0001] The invention relates to a method for promoting phytoremediation of soil compaction by using Acetobacter xylinum, belonging to the technical field of agriculture. Background technique [0002] Soil compaction is the phenomenon that the soil surface layer is destroyed by external factors such as rainfall or irrigation, and the soil material is dispersed, and then it is affected by the cohesive force after drying. The aggregate structure of soil is an important indicator of soil fertility. The destruction of soil aggregate structure will lead to the reduction of soil water retention, fertilizer retention capacity and permeability, resulting in soil compaction. [0003] Due to the extensive use of chemical fertilizers and pesticides in agricultural production, soil compaction has been caused, and the degree of salinization has intensified, posing a serious threat to the agricultural ecological environment. In particular, the special environmental conditi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/00B09C1/10
Inventor 崔青青
Owner 崔青青
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