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Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation

A technology of arbuscular mycorrhizal fungi and application method, applied in the field of biological pesticides, can solve problems such as patents or products that have not yet been cultivated in large areas, and achieve the effects of remarkable agronomic properties, improvement of mineral nutrition status, and simple and practical methods.

Inactive Publication Date: 2013-06-05
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many reports and patents on the application of arbuscular mycorrhizal fungus biotechnology in tobacco production, but most of them focus on the application and related supporting technologies in the process of seedling cultivation, and there are no patents or products applied to large-scale cultivation of tobacco

Method used

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  • Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation
  • Application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation

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Experimental program
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Embodiment 1

[0020] Embodiment 1: a kind of application method of arbuscular mycorrhizal fungus in tobacco large-scale cultivation, its implementation steps are as follows:

[0021] (1) Propagation of initial arbuscular mycorrhizal fungal agents:

[0022] Preparation of the initial arbuscular mycorrhizal fungal agent: purchased from the "Arbuscular mycorrhizal fungal germplasm in the resource bank" of Beijing Academy of Agriculture and Forestry Sciences, China Agricultural University and other scientific research institutions, the strains were Apophysis honeycolor ( Acaulospora mellea ), Glomus mosei ( Glomus mosseae ), Glomus intraradices ( Glomus intraradices) , containing arbuscular mycorrhizal fungal spores, hyphae and infected root segments.

[0023] The three initial arbuscular mycorrhizal fungal agents of A. honeycolor, G. mosesi and G. intrarhizogi were multiplied according to the following method: mix river sand and soil at a ratio of 3:1, and use clover as the ho...

Embodiment 2

[0027] Embodiment 2: a kind of application method of arbuscular mycorrhizal fungus in tobacco large-scale cultivation, its implementation steps are as follows:

[0028] (1) Propagation of initial arbuscular mycorrhizal fungal agents:

[0029] Preparation of the initial arbuscular mycorrhizal fungal agent: purchased from Beijing Academy of Agriculture and Forestry Sciences "Arbuscular mycorrhizal fungal germplasm in the resource bank", China Agricultural University and other scientific research institutions, the strains were Apophysis honeycolor ( Acaulospora mellea ), Glomus mosei ( Glomus mosseae ), Glomus intraradices ( Glomus intraradices ), containing arbuscular mycorrhizal fungal spores, hyphae and infected root segments.

[0030] The three initial arbuscular mycorrhizal fungal agents of A. honeycolor, Glomus mosesi and Glomus intrarhiza were respectively propagated according to the following method: river sand and soil were mixed at a ratio of 3:1, and c...

Embodiment 3

[0034] Embodiment 3: a kind of application method of arbuscular mycorrhizal fungus in tobacco large-scale cultivation, its implementation steps are as follows:

[0035] (1) Propagation of initial arbuscular mycorrhizal fungal agents:

[0036] Preparation of the initial arbuscular mycorrhizal fungal agent: purchased from Beijing Academy of Agriculture and Forestry Sciences "Arbuscular mycorrhizal fungal germplasm in the resource bank", China Agricultural University and other scientific research institutions, the strains were Apophysis honeycolor ( Acaulospora mellea ), Glomus mosei ( Glomus mosseae ), Glomus intraradices ( Glomus intraradices ), containing arbuscular mycorrhizal fungal spores, hyphae and infected root segments.

[0037] The three initial arbuscular mycorrhizal fungal agents of A. honeycolor, G. mosesi and G. intrarhizogi were multiplied according to the following method: mix river sand and soil at a ratio of 3:1, and use clover as the host Plan...

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Abstract

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.

Description

technical field [0001] The invention relates to an application method of arbuscular mycorrhizal fungi in large-area cultivation of tobacco seedlings, and belongs to the fields of resource utilization, agriculture, and biological pesticides. Background technique [0002] Arbuscular mycorrhizal fungi are the most widely distributed symbionts in terrestrial ecosystems and are also widespread in various contaminated soils. As we all know, arbuscular mycorrhizal fungi can improve the yield and quality of plants by improving the nutritional status of plants, especially phosphorus nutrition, and can also improve drought resistance, salt resistance, and heavy metal resistance. Numerous reports have shown that arbuscular mycorrhizal fungi technology has been widely used in tobacco production. For example, Chinese patent application No. 201210102348.2 invented the method of using mycorrhizal technology to improve tobacco drought resistance and disease resistance; Chinese patent appli...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01G7/00
Inventor 郭涛习向银
Owner SOUTHWEST UNIVERSITY
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