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Method for repairing glomus mosseae-contaminated soil through combination of glomus mosseae and nightshade-rice intercropping system

A technology for Glomus mosesi and cadmium-contaminated soil, applied in the field of phytoremediation, can solve the problems of high cost of remediation technology, difficult production practice, and long repair period, so as to improve the ability of absorbing and enriching cadmium, good repair effect, The effect of shortening the repair cycle

Inactive Publication Date: 2020-06-09
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing farmland soil heavy metal pollution remediation technology has high cost, single remediation agent, and long remediation period, so it is difficult to be used in production practice

Method used

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  • Method for repairing glomus mosseae-contaminated soil through combination of glomus mosseae and nightshade-rice intercropping system
  • Method for repairing glomus mosseae-contaminated soil through combination of glomus mosseae and nightshade-rice intercropping system
  • Method for repairing glomus mosseae-contaminated soil through combination of glomus mosseae and nightshade-rice intercropping system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1 Moses' balloon mold preparation

[0049] The arbuscular mycorrhizal fungus Glomus mosseae was provided by the Agricultural College of Guangxi University. In the present invention, corn (zea mays) is used as a host plant for potted propagation, and the mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potted sand is used as an inoculant.

[0050] Concrete preparation process is as follows:

[0051] S11. according to the inoculum amount of 3% by mass ratio, the arbuscular mycorrhizal fungus seed agent is placed in the 2 / 3 position of the aseptic basin with sterilized sand;

[0052] S12. Corn seeds with 10% H 2 o 2 Disinfect for 15-20 minutes, and soak the seeds for 12 hours; sow 10-15 corn seeds in each pot, cover with sterilized sand medium, and cultivate in the greenhouse;

[0053] The indoor temperature of the greenhouse is kept at 20-32° C., and the humidity is kept at 65%-75%.

[0054] S13. Cultivate potted pl...

Embodiment 2

[0057] Embodiment 2 Utilizes the method for joint restoration of cadmium-contaminated soil by Glomus mosesi and Solanum nigrum-rice intercropping system

[0058] 1. Experimental materials

[0059] (1) Solanum nigrum and upland rice as test plants

[0060] The test plant Solanum nigrum L. was sprayed with 10% H before sowing 2 o 2 Disinfect for 15-20 minutes, wash with deionized water, and then wash with 0.1% H 2 o 2 Soak the seeds for 12h. After soaking the seeds, spread the expanded seeds evenly in the sterilized quartz sand, and place them in a constant temperature incubator for cultivation. The test plant upland rice (Oryza sativa L.) was sprayed with 10% H before sowing 2 o 2 Sterilize for 15-20 minutes, wash with deionized water, soak the seeds in deionized warm water at 40-50°C for 10-12 hours, and after the seeds are white, sprinkle the seeds evenly in the sterilized quartz sand and place them in a constant temperature incubator nourish. The setting of the incu...

Embodiment 3

[0105] Embodiment 3 utilizes Glomus mosesi and Solanum nigrum-rice intercropping system to jointly remediate the method of cadmium-contaminated soil

[0106] The method for utilizing Glomus mosesi and Solanum nigrum-rice intercropping system to jointly restore cadmium-contaminated soil provided by the present embodiment comprises the following steps:

[0107] S1. Propagation and preparation of Moses balloon mold agent:

[0108] S11. According to the inoculum size of 1% according to the mass ratio, the Glomus moses strain agent is placed in the 4 / 5 position of the aseptic basin that the sterilized sand medium is housed;

[0109] S12. Sterilize the corn seeds for 15 minutes, soak the seeds for 10 hours, cover with sterilized sand medium, and cultivate them in a greenhouse where the indoor temperature is kept at 20° C. and the humidity is kept between 65%; the sand medium in S11 and S12 is fine River sand, the fine river sand is river sand passed through a 40-mesh sieve;

[011...

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Abstract

The invention discloses a method for repairing glomus mosseae-contaminated soil through combination of glomus mosseae and a nightshade-rice intercropping system. The method comprises the following steps: propagating to prepare a glomus mosseae inoculant; accelerating germination of the rice and solanum nigrum seeds to obtain rice and solanum nigrum seedlings; transplanting rice and solanum nigrumseedlings into the glomus mosseae-contaminated soil, wherein the intercropping ratio of solanum nigrum to rice is 1: (1-2); and adding the glomus mosseae inoculant into glomus mosseae contaminated soil to form a hyperaccumulator-dry rice-glomus mosseae symbiotic system. By inoculating glomus mosseae, the growth and soil Cd absorption capacity of solanum nigrum can be improved, meanwhile, the content of heavy metal cadmium in rice is remarkably reduced, the effect of repairing Cd-contaminated soil while safe production is achieved is achieved, and the method has the advantages of being efficient, low in cost, easy to operate, free of secondary pollution and the like and has good theoretical and application and popularization value.

Description

technical field [0001] The invention belongs to the technical field of phytoremediation in soil heavy metal restoration. Specifically, it relates to a method for remediating heavy metal cadmium-contaminated soil while producing; more specifically, it relates to a method for jointly remediating cadmium-contaminated soil by using Glomus mosei and nightshade-rice intercropping system. Background technique [0002] Soil heavy metal pollution has become one of the main environmental pollution problems in my country. According to the "National Soil Pollution Survey Bulletin" issued by the Ministry of Environmental Protection in 2014, the total point exceeding standard rate of soil in my country is 16.1%, and the exceeding standard rate of cadmium is 7.0%. At present, the remediation methods of heavy metal-contaminated soil can be divided into: physical method, chemical method, biological method, ecological method and soil agrochemical regulation method, or a combination of these ...

Claims

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Application Information

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IPC IPC(8): B09C1/10A01B79/02A01G22/22A01G22/00
CPCA01B79/02A01G22/00A01G22/22B09C1/105
Inventor 杨旭黎华寿秦俊豪李佳纯林榕楷
Owner SOUTH CHINA AGRI UNIV
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