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Method for plant-microbial remediation of heavy-metal polluted soil

A technology of combined restoration of polluted soil, applied in the field of soil restoration, can solve problems such as unsatisfactory soil restoration effect, soil microbial environment damage, poor plant growth, etc., achieve good restoration effect, shorten restoration period, and reduce heavy metal content Effect

Active Publication Date: 2017-09-05
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] However, this method can solve the contaminated soil with a low degree of heavy metal pollution to a certain extent, but the remediation effect on soil with a high degree of heavy metal pollution is not very satisfactory. The environment has been severely damaged, and many microorganisms have died due to heavy metal pollution, resulting in serious deterioration of soil conditions, resulting in severe poor growth of various plants, and it is even difficult to grow in this soil; at the same time, in the polluted soil, there are not only one Heavy metals often contain a variety of heavy metals. It is difficult to deal with the soil polluted by various heavy metals in the existing technology. question

Method used

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  • Method for plant-microbial remediation of heavy-metal polluted soil
  • Method for plant-microbial remediation of heavy-metal polluted soil
  • Method for plant-microbial remediation of heavy-metal polluted soil

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Experimental program
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Effect test

Embodiment 1

[0050] The method for the microorganism-plant joint remediation of heavy metal-contaminated soil provided in this embodiment comprises the following steps:

[0051] A: Preparation of mycorrhizal fungal inoculants: using sorghum to multiply the Pachymyces mosesi, Rhizocystis interrhizii, Glomus spp. The inoculum amount is 7% (weight ratio) of the propagation substrate, and the propagation time is 30 days to obtain 4 kinds of mycorrhizal fungus inoculants respectively; wherein, the propagation substrate is a sterilized mixture of river sand, vermiculite and soil; Among them, river sand, vermiculite and soil are mixed according to the weight ratio of 2:3:5.

[0052] A 1 : Screen and multiply the beneficial microorganisms in the area to be repaired: collect soil from the area to be repaired, screen and purify the beneficial microorganisms in the repaired area by the plate rapid detection method, and then use the medium to multiply to obtain the beneficial microorganisms in the ar...

Embodiment 2

[0062] The method for the microorganism-plant joint remediation of heavy metal-contaminated soil provided in this embodiment comprises the following steps:

[0063] A: preparation of mycorrhizal fungus inoculant: utilize sorghum to propagate respectively described Phanocystis moxie and Diocystis distortus in the propagation matrix, the inoculum size is 5% (weight ratio) of the propagation matrix, the expansion Propagation time is 30-60 days, obtains 2 kinds of mycorrhizal fungus inoculants respectively; Wherein, described propagation substrate is the sterilized mixture of river sand, vermiculite and soil; Wherein, river sand, vermiculite and soil according to 1:3: 5 weight ratio mix.

[0064] A 1 : Screen and multiply the beneficial microorganisms in the area to be repaired: collect soil from the area to be repaired, screen and purify the beneficial microorganisms in the repaired area by the plate rapid detection method, and then use the medium to multiply to obtain the benef...

Embodiment 3

[0073] The method for the microorganism-plant joint remediation of heavy metal-contaminated soil provided in this embodiment comprises the following steps:

[0074] A: Preparation of mycorrhizal fungal inoculants: using sorghum to multiply the Pachymyces mosesi, Rhizocystis interrhizii, Glomus spp. The inoculum size is 10% (weight ratio) of the propagation substrate, and the propagation time is 30-60 days to obtain 4 kinds of mycorrhizal fungal inoculants respectively; wherein, the propagation substrate is the sterilization of river sand, vermiculite and soil Mixture; wherein, river sand, vermiculite and soil are mixed according to the weight ratio of 2:2:5.

[0075] A 2 : Cultivation of actinomycetes microorganisms: screening and purification of actinomycetes microorganisms by plate rapid detection method. The microorganism of the genus Actinomyces is the genus Frankia. Among them, Frankia sp. ( 33255 TM ), (Accession number: Avcl1, depository unit: American Type Cultur...

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Abstract

The invention provides a method for plant-microbial remediation of heavy-metal polluted soil. The method includes the steps: A preparing mycorrhizal fungus inoculants; B performing symbiotic culture on the mycorrhizal fungus inoculants and seeds of host plants; C transplanting young seedlings into the heavy-metal polluted soil; D performing harvest after the growing period of the host plants finishes. According to the method, growth of the host plants is simulated under facilitation of the natural soil conditions, tolerance of the host plants for toxic substances is improved, various heavy metals in the soil are adsorbed, the content of the heavy metals in the soil is greatly reduced, remediation cost is remarkably reduced, remediation cycles are shortened, and the method has an excellent remediation effect on moderate or severe heavy-metal pollution.

Description

technical field [0001] The invention relates to soil remediation technology, in particular to a method for microorganism-plant joint remediation of heavy metal polluted soil. Background technique [0002] Antimony (Sb) is a toxic carcinogenic element, its content in the earth's crust is about 0.2-0.3mg / kg, and the soil background value is about 0.3-2.3mg / kg. Antimony and antimonides have been proven to be a class of genotoxic substances and carcinogenic to the human body. They are also a global pollutant with long-distance transmission characteristics. At present, the US Environmental Protection Agency and the Council of the European Community have Antimony is listed as a priority pollutant. With the development of modern industry, the demand for antimony has increased sharply. Most of the antimony released into the environment is enriched and transmitted in terrestrial ecosystems, and accumulates in the human body through the food chain, which eventually affects the metabo...

Claims

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Application Information

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IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/10B09C1/105B09C2101/00
Inventor 魏源沈亚琴侯红陈志鹏孙在金吴丰昌朱元荣白英臣汤志
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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