Method for promoting sprouting of arbuscular mycorrhizal fungi spore and growth of hypha

A technology for arbuscular mycorrhizal fungi and spore germination, applied in the direction of using spores, microorganism-based methods, biochemical equipment and methods, etc., and can solve problems such as no biological factors

Inactive Publication Date: 2014-08-13
ZHEJIANG NORMAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

Storing such a large amount of genetic information and energy is a selective advantage of this species, but it is clear that the spores of AM fungi d

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  • Method for promoting sprouting of arbuscular mycorrhizal fungi spore and growth of hypha
  • Method for promoting sprouting of arbuscular mycorrhizal fungi spore and growth of hypha
  • Method for promoting sprouting of arbuscular mycorrhizal fungi spore and growth of hypha

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Embodiment Construction

[0018] The present invention will be described in detail below with reference to the drawings and specific embodiments.

[0019] Compared with the prior art, the present invention has the following beneficial effects: simple materials and equipment, easy implementation, low cost, and environmental friendliness. This technology promotes the rapid germination of arbuscular mycorrhizal fungi spores and the rapid derivation of hyphae growth. The original medium for spore germination, water agar is used in the traditional method, but this is not conducive to the lack of nutrients during the later growth of spores. Influencing its further symbiosis with plants, this experiment provides a favorable condition that not only shortens the spore germination time period, but also improves the germination rate and promotes the increase of the hyphae length. The high spore germination rate and faster hyphae growth The speed guarantees the efficient use of biological fertilizer in agriculture.

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Abstract

The invention discloses a method for promoting sprouting of arbuscular mycorrhizal fungi spore and growth of hypha. The method comprises the steps: screening arbuscular mycorrhizal fungi spores, sterilizing the arbuscular mycorrhizal fungi spores, sucking the sterilized spores by utilizing a sterile gun head under the microscope, transferring the spores onto a culture medium in a culture dish, inoculating 50 spores in each culture dish, culturing in a dark box, and culturing the spores for 20 days under the condition of 26 DEG C. The method has beneficial effects that the glomus intraradices spore sprouting and hypha growth are promoted by adopting 50ml/L root secreted liquid of RiT-DNA transgenic carrot root, glucose and an appropriate nitrogen source. The method has the advantages of simple and convenient operation, use of cheap materials and environmental friendliness.

Description

Technical field [0001] The invention belongs to the technical field of fungal germination and hyphae derivation promoters, and relates to a method for promoting arbuscular mycorrhizal fungal spore germination and hyphae derivation. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) are the most common fungi that live on land and have an underground symbiotic relationship with the most extensive plant species. Before forming a symbiotic relationship with a host plant in the soil, arbuscular mycorrhizal fungi undergo a pre-symbiotic phase, including spore germination, hyphae growth, and branch formation; when host roots are present, they invade again with the formation of attached bubbles Into the root and extend between the cells to form an arbuscular structure, then the exophytic hyphae grow, and finally spores are formed. The spores of AM fungi are very large and include many cell nuclei, storing large amounts of fat and sugars. Storing such a large amount of gen...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00C12R1/645
Inventor 金海如赵腊梅
Owner ZHEJIANG NORMAL UNIVERSITY
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