Application and method for water conservation and yield increase of dry land wheat through cooperation of nano elemental iron and arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungi and nano-elements, applied in application, botanical equipment and methods, planting substrate, etc., can solve problems such as unclear influence, and achieve the effects of fertilizing soil, increasing yield and promoting wheat growth

Pending Publication Date: 2022-07-29
GANSU ACAD OF SCI INST OF BIOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many strains of AMF, and whether the addition of a certain strain in AMF can further promote the growth of wheat on the basis of nZVI, and play a role in improving wheat yield is not yet clear. The coexistence of the two

Method used

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  • Application and method for water conservation and yield increase of dry land wheat through cooperation of nano elemental iron and arbuscular mycorrhizal fungi
  • Application and method for water conservation and yield increase of dry land wheat through cooperation of nano elemental iron and arbuscular mycorrhizal fungi
  • Application and method for water conservation and yield increase of dry land wheat through cooperation of nano elemental iron and arbuscular mycorrhizal fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A plastic pot with a diameter of 26 cm and a height of 23 cm was used as the culture vessel. The spring wheat cv.LC29 was used as the cultivation crop; the mixture of vermiculite and local soil soil (the volume ratio of vermiculite and local soil soil was 1:2) was used as the cultivation substrate. Before inoculation, dry heat sterilization was carried out at 160°C for 4 h on the cultivation substrate used, and the greenhouse and culture vessel were sterilized with pasteurized solution.

[0027] Pick plump, uniform and healthy wheat seeds, disinfect them with sodium hypochlorite with a concentration of 0.1% for 2 minutes, then wash them with distilled water, and soak the seeds with nZVI aqueous solutions of 5mg / L, 10mg / L and 15mg / L concentrations for 4h respectively.

[0028] White clover was used to propagate Gi in sterilized sandy soil (river sand: soil mixed at a ratio of 1:1, sterilized at 160° C. for 4 h at high temperature). The amount of Gi inoculum is 3% to 5% ...

Embodiment 2

[0045] Two-year field experiments were carried out. The field experiments were controlled by environmental conditions, with 10mg / LnZVI and Gi co-treatment. At the same time, 10mg / L nZVI seed soaking, Gi inoculation and CK treatment were carried out. Each plot was designed to be 5m×5m=25m 2 The total area is 10 rows per cell, the row spacing is 0.2m, and there is a 0.5m buffer zone between adjacent cells. Sow spring wheat seeds per row, 1000 plants per plot. Seeds were soaked in water and 10 mg / L nZVI before sowing, and 5 g Gi or inactivated Gi was added to each row. Fertilize and sow after soil preparation. Phosphorus and nitrogen fertilizers are applied once a year as base fertilizers at a rate of 150kgP ha. -1 and 225kg Nha -1 .

[0046] The effects of different treatments on the growth, yield and water use efficiency of dryland wheat in two years are shown in Table 3:

[0047] Table 3 Effects of different treatments on the growth, yield and water use efficiency of dryla...

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Abstract

The invention discloses application and method for water saving and yield increasing of dry land wheat through cooperation of nano elemental iron and arbuscular mycorrhizal fungi, and in the wheat planting process, nZVI and AMF are jointly used for treatment, so that the water utilization efficiency and yield of the dry land wheat can be remarkably improved, and glomus intraradices is adopted as the arbuscular mycorrhizal fungi; and propagating the glomus intraradices to obtain glomus intraradices inoculum containing 150-200 spores/10g of dry soil, hyphae inside and outside roots and infected root segments. The method comprises the following steps: firstly, carrying out seed soaking treatment by utilizing a solution of nano elemental iron, then adding a glomus intraradices inoculum or an inactivated glomus intraradices inoculum into a culture medium or field soil, and then sowing the wheat seeds subjected to seed soaking treatment. The symbiotic relationship between the AMF and the dry land wheat is enhanced through the nZVI, the growth of the wheat is promoted by utilizing the synergistic effect of the nZVI and the glomus intraradices inoculum, the water utilization efficiency and the drought adaptability of host plants are improved, and the method is beneficial to coping with the drought and changeable climatic environment.

Description

technical field [0001] The invention belongs to the technical field of nano-ecological agriculture, and in particular relates to an application and method for water-saving and yield-increasing of dryland wheat in synergy with nano-element iron and arbuscular mycorrhizal fungi. Background technique [0002] my country belongs to a large agricultural country. Wheat is one of the three major grain crops, and its output is second only to corn, ranking second; because wheat is rich in starch, protein, fat, minerals, calcium, iron, thiamine, riboflavin , niacin, vitamin A and vitamin C and other nutrients, the output is almost all edible; the output of wheat is related to food security and human health. Nano-ecological agriculture is an interdisciplinary field proposed in recent years with huge application potential, providing innovative ideas for farmland ecosystem sustainability and food security. Engineered nanoparticles have been widely used in agricultural production in recen...

Claims

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Application Information

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IPC IPC(8): A01G22/20A01C1/00A01G24/00
CPCA01G22/20A01C1/00A01G24/00
Inventor 祝英熊友才米哈纳西闫鹏勋吴志国
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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