Method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi

An arbuscular mycorrhizal fungus and banana technology, applied in the field of gardening, can solve the problems of soil structure damage in banana orchards, affecting the growth of banana plants, low microbial community vitality, etc. Developmental improvement effect

Active Publication Date: 2020-09-11
POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional banana cultivation methods rely heavily on a large amount of chemical fertilizers, which often lead to problems such as soil structure damage, soil compaction, decreased organic matter content, and low microbial community activity in banana orchards, which significantly affect the growth of bananas, especially seedling plants.

Method used

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  • Method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi
  • Method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi
  • Method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the impact of inoculating arbuscular mycorrhizal fungi on banana plant biomass

[0023] The experiment set up 4 experimental groups: control group (CK, not inoculated with arbuscular mycorrhizal fungus), treatment 1 (T1, inoculated with 100% concentration of Glomus intraradices), treatment 2 (T2, inoculated with 100% concentration of cassava Blastomyces) and treatment 3 (T3, inoculate after mixing 100% concentration of Glomus intraradices and 100% concentration of Glomus cassava according to the spore ratio of 1:1).

[0024] Wherein, the specific steps of processing 3 are: wash the medium in the banana tissue culture seedlings (5 leaf ages, the Brazilian banana tissue culture seedlings with the same growth) with clear water, soak the roots of the banana tissue culture seedlings in the arbuscular mycorrhizal fungus Mixed strain inoculum (preparation: activate Glomus intraradices and Glomus cassava respectively in a 25°C incubator in the dark, and then use p...

Embodiment 2

[0028] Embodiment 2: the impact of inoculating arbuscular mycorrhizal fungi on the number of soil microorganisms of banana seedlings

[0029] The experimental design was the same as in Example 1, and each group was repeated in 5 pots, with 1 banana seedling in each pot, 20 pots in total. Three months after the banana seedlings were planted, 5 points evenly distributed on the circumference of 5-6 cm away from the main trunk of the plant were selected, and 8 g of soil samples about 3-4 cm away from the surface were collected at each point, and the 5 samples of the experimental group were evenly mixed, and used in soil microbiological assays. Bacteria, fungi, actinomycetes, ammonifying bacteria and nitrogen-fixing bacteria in the soil were respectively used beef extract peptone medium (recipe: beef extract 3.0g, peptone 10.0g, NaCl 5.0g, agar 20g, water 1000mL, pH 7.4-7.6 , preparation steps: dissolve each component in water, stir and mix, adjust pH, sterilize, and obtain), Mart...

Embodiment 3

[0031] Embodiment 3: the influence of inoculating arbuscular mycorrhizal fungi on the growth of banana plants

[0032] As can be known from Example 2, inoculation of arbuscular mycorrhizal fungus mixed strains can increase the number of microorganisms in the soil. After the growth of banana seedlings for three months, we have 5 strains of banana seedlings in each experimental group. Plant height, stem girth Indexes such as (upper, middle and lower), green leaf number, maximum leaf length, maximum leaf width are averaged after measuring, and the results are as shown in table 2; and C, N, K, P of each experimental group plant blade The content of the elements was determined, and the results are shown in Table 3.

[0033] Table 2 Growth of banana seedlings under different inoculation treatments

[0034]

[0035]Table 3 Comparison of elements C, N, K, and P in leaves of plants with different inoculation treatments

[0036]

[0037] The experimental results show that the ba...

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Abstract

The invention discloses a method for promoting growth of banana seedlings by inoculating arbuscular mycorrhizal fungi. According to the method, it is found that an arbuscular mycorrhizal fungus mixedstrain (glomus intraradices and glomus manihotis are mixed at a spore ratio of 1:1) has a significant improvement effect on growth and development of banana seedlings as an efficient and safe biological fertilizer and has better ecological economic benefits and environment sustainability effect than other biological fertilizers as a renewable resource for sustainable development through inoculating the arbuscular mycorrhiza fungus mixed strain into banana roots. The arbuscular mycorrhiza fungus mixed strain has a promotion effect on growth of bacteria, fungi, actinomycetes, nitrogen-fixing bacteria and ammonifiers in a culture substrate, and plays an obvious optimization role in metabolic activity of soil microbial communities of the banana seedlings three months after being inoculated, and growth of banana tissue culture seedlings is significantly promoted by a large number of bacterial metabolic activities.

Description

technical field [0001] The invention relates to the technical field of gardens, in particular to a method for promoting the growth of banana seedlings by inoculating arbuscular mycorrhizal fungi. Background technique [0002] Banana belongs to the Musa family (Musaceae) and is an important food crop and tropical and subtropical fruit in the world. It is mainly distributed in Guangdong, Guangxi, Hainan, Yunnan and Fujian provinces (regions) in China. In 2017, my country's banana planting area was 5.9 million mu, the harvested area was 4.95 million mu, and the output was 12.5 million tons. It is one of the important economic crops for farmers in tropical regions to increase their income. However, traditional banana cultivation methods rely heavily on large amounts of chemical fertilizers, which often lead to problems such as soil structure damage, soil compaction, decreased organic matter content, and low microbial community activity in banana orchards, which significantly aff...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/05A01G7/06A01N63/30A01P21/00
CPCA01G22/05A01G7/06A01N63/30
Inventor 董涛窦同心何维第胡春华高慧君
Owner POMOLOGY RES INST GUANGDONG ACADEMY OF AGRI SCI
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