Rice seed seedling raising method based on combination of glomus intraradices and agrobacterium rhizogenes

A technology of intra-rhizobacterial fungus and Agrobacterium rhizogenes, which is applied in the directions of seed coating/seed dressing, rice cultivation, seed and rhizome treatment, etc., can solve the problems of poor application effect, etc. Pesticide and chemical fertilizer application rate, the effect of promoting growth and development

Pending Publication Date: 2021-08-06
HEILONGJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to solve the problem that the effect of the existing biological fertilizer is limited to the non-flooding period of rice, and the application

Method used

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specific Embodiment approach 1

[0007] Specific embodiment one: the present embodiment carries out the method for paddy seedling raising based on the combination of Glomus intraradices and A. 600 value of 0.6 to 0.8) diluted to 90 to 110 mL / L of Agrobacterium rhizogenes inoculum, soak the seeds in the inoculum for 8 to 16 hours, filter and then combine with fungal spores with a content greater than 25 / g The root capsule mold agent and the rice seedling-raising substrate are mixed evenly, and the seeds are sown, and the rice seedlings are raised.

[0008] The Agrobacterium rhizogenes in the present embodiment is a liquid inoculum, and the Agrobacterium rhizogenes inoculum is a pure bacterial liquid of Agrobacterium rhizogenes (bacterial liquid OD 600 The value is 0.6-0.8) diluted with sterile water to a liquid bacterial agent with a concentration of 90-110mL / L, and the bacterial agent is provided by the Key Laboratory of Ecological Restoration and Resource Utilization in Cold Regions of Heilongjiang Province....

specific Embodiment approach 2

[0011] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the weight ratio of rice seeds to Agrobacterium rhizogenes is 1:1.5-2.5. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0012] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that the weight ratio of rice seeds to Agrobacterium rhizogenes is 1:2. Other steps and parameters are the same as those in the first embodiment.

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Abstract

The invention discloses a rice seed seedling raising method based on combination of glomus intraradices and agrobacterium rhizogenes, relates to a rice seed seedling raising method, and solves the problems that the effect of existing biological fertilizer is only limited to the rice non-flooding period, and the application effect is poor. The method comprises the following steps: accelerating germination of rice seeds, soaking the rice seeds in an agrobacterium rhizogenes agent, filtering, uniformly mixing the rice seeds with the glomus intraradices agent and a rice seedling substrate, and carrying out sowing. According to the seedling raising method, hairy roots generated by agrobacterium rhizogenes induced rice and rice protogenetic root systems can be infected, bacterium and root symbionts can be formed, vitality can be continuously kept after the rice is transplanted to a paddy field, nutrient substances are continuously provided for the rice, growth and development of the rice are promoted, the yield is increased, the blast resistance is enhanced, the application amount of pesticides and chemical fertilizers in the paddy field is reduced, and the ecological agriculture is further developed while the rice production cost is reduced.

Description

technical field [0001] The invention relates to a method for growing seedlings of rice seeds. Background technique [0002] Nitrogen and phosphorus are a large number of elements necessary for the growth and development of rice. At present, the high yield of rice depends on the excessive use of chemical fertilizers, which has led to the aggravation of agricultural environmental pollution in most rice fields. At present, my country is facing three major soil problems: slow transformation of soil nutrients, accumulation of soil pollutants, and frequent occurrence of soil-borne diseases. This situation determines that China needs to develop and use biological fertilizers more than ever. However, the bio-fertilizers currently used are limited to the non-flooding period of rice, and lose their efficacy after being transplanted to paddy fields. The duration of fertilizer effect is short and the effect is not good, so it is difficult to have better application in paddy field produ...

Claims

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Application Information

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IPC IPC(8): A01G22/22A01C1/00A01C1/06
CPCA01G22/22A01C1/00A01C1/06
Inventor 宋福强张博常伟范晓旭
Owner HEILONGJIANG UNIV
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